Construct: sgRNA BRDN0001149092
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCAGAACGGAGGGCCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TKFC (26007)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75786
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
61338045 |
- |
TKFC |
NNNCAGAACGGAGGGCCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
47998979 |
- |
ELSPBP1 |
NNNCAGAAGGGAGGGCCACA |
NGG |
2 |
0.5804 |
Tier II |
| 3 |
NC_000009.12 |
9 |
133274421 |
- |
ABO |
NNNCAGAACGCAGGGCCACG |
NGG |
1 |
0.4286 |
Tier II |
| 4 |
NC_000016.10 |
16 |
72901792 |
+ |
ZFHX3 |
NNNCAGAACGGACGGCCACG |
NGG |
1 |
0.4211 |
Tier II |
| 5 |
NC_000004.12 |
4 |
42132169 |
- |
BEND4 |
NNNCAGAACCGAGGGACACG |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000021.9 |
21 |
45540778 |
+ |
SLC19A1 |
NNNCAGACAGGAGGGCCACG |
NGG |
2 |
0.3673 |
Tier II |
| 7 |
NC_000022.11 |
22 |
20245192 |
- |
RTN4R |
NNNCAGAACTGAGCGCCACG |
NGG |
2 |
0.3482 |
Tier II |
| 8 |
NC_000015.10 |
15 |
63371406 |
+ |
CA12 |
NNNCAGAGCCGAGGGCCACG |
NGG |
2 |
0.2933 |
Tier II |
| 9 |
NC_000022.11 |
22 |
46589948 |
- |
GRAMD4 |
NNNCAGCAGGGAGGGCCACG |
NGG |
2 |
0.2708 |
Tier II |
| 10 |
NC_000010.11 |
10 |
19906339 |
+ |
PLXDC2 |
NNNCAGTACGCAGGGCCACG |
NGG |
2 |
0.1875 |
Tier II |
| 11 |
NC_000001.11 |
1 |
202268213 |
- |
LGR6 |
NNNCTGCACGGAGGGCCACG |
NGG |
2 |
0.1591 |
Tier II |
| 12 |
NC_000007.14 |
7 |
157561659 |
+ |
PTPRN2 |
NNNCAGAACGGAGGGCCCCT |
NGG |
2 |
0.1333 |
Tier II |
| 13 |
NC_000002.12 |
2 |
109524103 |
- |
RANBP2 |
NNNCAGAACGGAGGCCCACG |
NAG |
2 |
0.0707 |
Tier II |
| 14 |
NC_000002.12 |
2 |
25246956 |
- |
DNMT3A |
NNNCAGAACGGAGGCCCAGG |
NGG |
2 |
0.0341 |
Tier II |
| 15 |
NC_000004.12 |
4 |
25879003 |
- |
LOC102723733 |
NNNCAGAACTGGGGGCCACG |
NGG |
2 |
0.5868 |
Tier III |
| 16 |
NC_000002.12 |
2 |
174535327 |
+ |
LOC124906096 |
NNNCAGAAGGGTGGGCCACG |
NGG |
2 |
0.2063 |
Tier III |
| 17 |
NC_000002.12 |
2 |
174535327 |
+ |
LOC124906097 |
NNNCAGAAGGGTGGGCCACG |
NGG |
2 |
0.2063 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
10600657 |
+ |
Tkfc |
NNNCAGAACGTAGGGCCACA |
NGG |
2 |
0.3606 |
Tier I |
| 2 |
NC_000080.6 |
14 |
35230632 |
- |
Grid1 |
NNNCAGAATGGAGGGCCATG |
NGG |
2 |
0.4038 |
Tier II |
| 3 |
NC_000085.6 |
19 |
16620240 |
- |
Vps13a |
NNNCAGACCGGAGGGCCACA |
NGG |
2 |
0.4018 |
Tier II |
| 4 |
NC_000069.6 |
3 |
82074457 |
- |
Gucy1b1 |
NNNCAGAGCGGAGGGCCGCG |
NGG |
2 |
0.2933 |
Tier II |
| 5 |
NC_000077.6 |
11 |
63901670 |
+ |
Hs3st3b1 |
NNNCAGAACGGAAGGTCACG |
NGG |
2 |
0.284 |
Tier II |
| 6 |
NC_000077.6 |
11 |
79593617 |
- |
Rab11fip4 |
NNNCAGAACGGAGGGTCTCG |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000067.6 |
1 |
76050355 |
+ |
Gm32622 |
NNNCAGAACGGAGGGGCAAG |
NGG |
2 |
0.0659 |
Tier III |
Other clones with same target sequence:
(none)
