Construct: sgRNA BRDN0001149121

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTCAAGTCATCCAACTGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPR1 (4881)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77304

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 153687261 + NPR1 NNNCAAGTCATCCAACTGCG NGG 0 1.0 Tier I
2 NC_000009.12 9 35805572 + NPR2 NNNCAAGTCCTCCAACTGTG NGG 2 0.1538 Tier I
3 NC_000001.11 1 230788992 - CAPN9 NNNCAAGTCATCCAACTACT NGG 2 0.4846 Tier II
4 NC_000007.14 7 148028365 - CNTNAP2 NNNCAAGTCATCCAAATGTG NGG 2 0.4615 Tier II
5 NC_000001.11 1 22076231 - CDC42 NNNCAAGTAATCCAACTGCC NGG 2 0.3673 Tier II
6 NC_000016.10 16 71693964 - PHLPP2 NNNCAAGTGATCCAACTGCC NGG 2 0.2653 Tier II
7 NC_000002.12 2 152392607 + FMNL2 NNNCAAGTCATCCAAATGCG NAG 2 0.2593 Tier II
8 NC_000016.10 16 70178505 + CLEC18C NNNCAAGTGATCCACCTGCG NGG 2 0.1688 Tier II
9 NC_000016.10 16 74417035 - CLEC18B NNNCAAGTGATCCACCTGCG NGG 2 0.1688 Tier II
10 NC_000001.11 1 10546337 - PEX14 NNNCAAGTCATCCACCTGCC NGG 2 0.1169 Tier II
11 NC_000001.11 1 51327944 + TTC39A NNNCAAGTCATCCACCTGCC NGG 2 0.1169 Tier II
12 NC_000016.10 16 79193576 + WWOX NNNCCAGTCATCCATCTGCG NGG 2 0.1 Tier II
13 NC_000007.14 7 34019052 - BMPER NNNCAAGTCATCCATCTGTG NGG 2 0.0923 Tier II
14 NC_000002.12 2 36882538 - STRN NNNCAAGTGATCCAACTGCG NCG 2 0.0663 Tier II
15 NC_000015.10 15 63293041 - APH1B NNNCAAGTCATTCAACTGCG NCG 2 0.0577 Tier II
16 NC_000001.11 1 20403562 - LINC01141 NNNCAAGACATCCAACTGCA NGG 2 0.75 Tier III
17 NC_000001.11 1 230788992 - LOC107985359 NNNCAAGTCATCCAACTACT NGG 2 0.4846 Tier III
18 NC_000007.14 7 112349465 - ZNF277-AS1 NNNCCAGTCATCCAACTGCA NGG 2 0.4688 Tier III
19 NC_000021.9 21 33967836 - LINC00649 NNNCAAGTGATCCAACTGCT NGG 2 0.4333 Tier III
20 NC_000021.9 21 33967836 - LOC124905013 NNNCAAGTGATCCAACTGCT NGG 2 0.4333 Tier III
21 NC_000013.11 13 94929401 - LOC101927284 NNNCAAGTCATCTAACTGCC NGG 2 0.3 Tier III
22 NC_000010.11 10 45234902 + CUBNP2 NNNCAAGTGATCCAACTGCC NGG 2 0.2653 Tier III
23 NC_000016.10 16 71693964 - LOC124903712 NNNCAAGTGATCCAACTGCC NGG 2 0.2653 Tier III
24 NC_000002.12 2 39574661 + MAP4K3-DT NNNCAAGTAATCCACCTGCG NGG 2 0.2338 Tier III
25 NC_000005.10 5 77954966 - LOC101929154 NNNCAAGTGATCCACCTGCG NGG 2 0.1688 Tier III
26 NC_000012.12 12 102486261 - LINC02456 NNNCAAGTCATCCAACTGTG NGA 2 0.0321 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 90458168 - Npr1 NNNCAAGTCATCCAACTGCG NGG 0 1.0 Tier I
2 NC_000074.6 8 95534249 + Ccdc113 NNNCCAGTCATCCAACTGTG NGG 2 0.2308 Tier I
3 NC_000070.6 4 43646602 + Npr2 NNNCAAGTCCTCCAACTGTG NGG 2 0.1538 Tier I
4 NC_000072.6 6 97517551 + Frmd4b NNNCAAGTCTTTCAACTGCG NGG 2 0.4751 Tier II
5 NC_000080.6 14 93771571 + Pcdh9 NNNCAAGTCATCCAACTTCT NGG 2 0.4667 Tier II
6 NC_000080.6 14 34867281 - Grid1 NNNCAAGTGATACAACTGCG NGG 2 0.4422 Tier II
7 NC_000078.6 12 87463515 + Snw1 NNNCAAGTCATCCGACTGCT NGG 2 0.3267 Tier II
8 NC_000073.6 7 88294631 - Ctsc NNNCAAGTCATCCAAGTGCC NGG 2 0.0659 Tier II
9 NC_000086.7 X 78390881 + Gm8754 NNNCAAGTCATACAACTGCA NGG 2 0.6696 Tier III
10 NC_000072.6 6 97517551 + Gm46970 NNNCAAGTCTTTCAACTGCG NGG 2 0.4751 Tier III
11 NC_000070.6 4 45739723 - Gm31563 NNNCAAGTCCTCCAACTGCT NGG 2 0.2333 Tier III
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Other clones with same target sequence:

(none)