Construct: sgRNA BRDN0001149125
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAAAACGATCAAACCCCAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- JAK2 (3717)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75728
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
5050807 |
+ |
JAK2 |
NNNAAACGATCAAACCCCAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
5050807 |
+ |
INSL6 |
NNNAAACGATCAAACCCCAC |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000016.10 |
16 |
67566875 |
- |
CTCF |
NNNAAACAAACAAACCCCAC |
NGG |
2 |
0.8571 |
Tier II |
| 4 |
NC_000013.11 |
13 |
25646838 |
- |
ATP8A2 |
NNNAAACGATCAAACCACAA |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000003.12 |
3 |
171357720 |
+ |
TNIK |
NNNAAACCATCAAACCCCAT |
NGG |
2 |
0.1846 |
Tier II |
| 6 |
NC_000009.12 |
9 |
3436795 |
+ |
RFX3 |
NNNAAACGATCACACCCAAC |
NGG |
2 |
0.1134 |
Tier II |
| 7 |
NC_000007.14 |
7 |
4222113 |
+ |
SDK1 |
NNNAAACCATCAAACCCCAC |
NTG |
2 |
0.024 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
89139666 |
- |
Sh3bp4 |
NNNAAACAAACAAACCCCAC |
NGG |
2 |
0.8571 |
Tier II |
| 2 |
NC_000081.6 |
15 |
55865328 |
- |
Sntb1 |
NNNAAACAAACAAACCCCAC |
NGG |
2 |
0.8571 |
Tier II |
| 3 |
NC_000072.6 |
6 |
71844535 |
- |
Immt |
NNNAAACGATCAAACACTAC |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000074.6 |
8 |
107530314 |
- |
Wwp2 |
NNNAAACATTCAAACCCCAC |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000075.6 |
9 |
28371870 |
+ |
Opcml |
NNNAAACAAGCAAACCCCAC |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000071.6 |
5 |
136245849 |
+ |
Sh2b2 |
NNNAAACGACAAAACCCCAC |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000070.6 |
4 |
136195888 |
- |
E2f2 |
NNNAAATGATCAAACCCCAC |
NAG |
2 |
0.2106 |
Tier II |
| 8 |
NC_000077.6 |
11 |
42628215 |
- |
Gabrb2 |
NNNAAATGATCAAACCCCAC |
NGA |
2 |
0.0564 |
Tier II |
| 9 |
NC_000084.6 |
18 |
42083846 |
- |
Sh3rf2 |
NNNAAACCATCAAACCCCAC |
NGA |
2 |
0.0427 |
Tier II |
| 10 |
NC_000084.6 |
18 |
75389298 |
- |
Smad7 |
NNNAAACGATCAAATCCCAC |
NGC |
2 |
0.0015 |
Tier II |
| 11 |
NC_000075.6 |
9 |
28371870 |
+ |
Gm39313 |
NNNAAACAAGCAAACCCCAC |
NGG |
2 |
0.5 |
Tier III |
| 12 |
NC_000071.6 |
5 |
136245849 |
+ |
Gm30408 |
NNNAAACGACAAAACCCCAC |
NGG |
2 |
0.4 |
Tier III |
| 13 |
NC_000070.6 |
4 |
9805305 |
+ |
4930412C18Rik |
NNNATACGATCAAATCCCAC |
NGG |
2 |
0.0242 |
Tier III |
Other clones with same target sequence:
(none)
