Construct: sgRNA BRDN0001149126
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGCTGTGAAGATCCCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK5 (2869)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76271
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
119442042 |
+ |
GRK5 |
NNNGCTGTGAAGATCCCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
2362702 |
+ |
EIF3B |
NNNGCTGTGAAGAACGCCGA |
NGG |
2 |
0.0952 |
Tier I |
| 3 |
NC_000001.11 |
1 |
45347927 |
- |
TESK2 |
NNNGCTGAGAAGATCCCCGA |
NGT |
2 |
0.0129 |
Tier I |
| 4 |
NC_000001.11 |
1 |
110955637 |
+ |
LRIF1 |
NNNGCTGTGAAGATCACTGA |
NGG |
2 |
0.6429 |
Tier II |
| 5 |
NC_000003.12 |
3 |
21418248 |
- |
ZNF385D |
NNNGCTGTGAAAATCCCTGA |
NGG |
2 |
0.6 |
Tier II |
| 6 |
NC_000008.11 |
8 |
142298085 |
+ |
TSNARE1 |
NNNGCTGTGAGGGTCCCCGA |
NGG |
2 |
0.4239 |
Tier II |
| 7 |
NC_000007.14 |
7 |
158165030 |
+ |
PTPRN2 |
NNNGCAGTGAAGACCCCCGA |
NGG |
2 |
0.2476 |
Tier II |
| 8 |
NC_000007.14 |
7 |
158165162 |
+ |
PTPRN2 |
NNNGCAGTGAAGACCCCCGA |
NGG |
2 |
0.2476 |
Tier II |
| 9 |
NC_000019.10 |
19 |
1220925 |
- |
STK11 |
NNNTCTGTGAAGATCCCTGA |
NGG |
2 |
0.2338 |
Tier II |
| 10 |
NC_000002.12 |
2 |
240519196 |
- |
ANKMY1 |
NNNGCTGTGAAGATTCCAGA |
NGG |
2 |
0.0359 |
Tier II |
| 11 |
NC_000002.12 |
2 |
230952699 |
- |
GPR55 |
NNNGCTGTGCAGATGCCCGA |
NGG |
2 |
0.0167 |
Tier II |
| 12 |
NC_000017.11 |
17 |
64608812 |
- |
SMURF2 |
NNNGCTGTGAAGATCCCCGA |
NAA |
2 |
0.0 |
Tier II |
| 13 |
NC_000023.11 |
X |
35985853 |
- |
CFAP47 |
NNNGCTGTGTAGAGCCCCGA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000011.10 |
11 |
75711396 |
- |
LOC105369392 |
NNNGCTGTGAAGGTCCCCCA |
NGG |
2 |
0.2924 |
Tier III |
| 15 |
NC_000013.11 |
13 |
106330008 |
+ |
LOC107984626 |
NNNGCTGTGAAGGTCCCCGA |
NGA |
2 |
0.0453 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
61080897 |
+ |
Grk5 |
NNNGCCGTGAAGATCCCCGA |
NGG |
1 |
0.9091 |
Tier I |
| 2 |
NC_000074.6 |
8 |
124673667 |
- |
Ttc13 |
NNNGCTGGGAAGATCCCCAA |
NGG |
2 |
0.5238 |
Tier I |
| 3 |
NC_000067.6 |
1 |
66622490 |
+ |
Unc80 |
NNNGCTGTGAAGGTCCCCGA |
NGC |
2 |
0.0145 |
Tier I |
| 4 |
NC_000085.6 |
19 |
61080897 |
+ |
Zfp950 |
NNNGCCGTGAAGATCCCCGA |
NGG |
1 |
0.9091 |
Tier II |
| 5 |
NC_000074.6 |
8 |
69838819 |
+ |
Pbx4 |
NNNGCTGTGAAGATCACCCA |
NGG |
2 |
0.4483 |
Tier II |
| 6 |
NC_000084.6 |
18 |
46873344 |
- |
Lvrn |
NNNGCTGAGAAGATCCCAGA |
NGG |
2 |
0.4308 |
Tier II |
| 7 |
NC_000084.6 |
18 |
23513436 |
+ |
Dtna |
NNNGCTGTTAAGATCCCTGA |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000070.6 |
4 |
156195821 |
- |
Agrn |
NNNGCTGTGAAGATCCCAGC |
NGG |
2 |
0.1224 |
Tier II |
| 9 |
NC_000071.6 |
5 |
96542064 |
+ |
Fras1 |
NNNGCTGTGAAGATCCCTGA |
NGA |
2 |
0.0446 |
Tier II |
| 10 |
NC_000070.6 |
4 |
156195821 |
- |
Gm46878 |
NNNGCTGTGAAGATCCCAGC |
NGG |
2 |
0.1224 |
Tier III |
| 11 |
NC_000067.6 |
1 |
176463516 |
- |
Gm36536 |
NNNGCTGTGAAGATCCCCTA |
NGA |
2 |
0.0463 |
Tier III |
Other clones with same target sequence:
(none)
