Construct: sgRNA BRDN0001149127
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCCTGAGCCCCGACTACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK5 (2869)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76273
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
119443590 |
+ |
GRK5 |
NNNCTGAGCCCCGACTACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
47421369 |
+ |
MEIS3 |
NNNCTGAGCACCGACCACTG |
NGG |
2 |
0.5778 |
Tier II |
| 3 |
NC_000016.10 |
16 |
306650 |
- |
AXIN1 |
NNNCTGAGCCTCGTCTACTG |
NGG |
2 |
0.1641 |
Tier II |
| 4 |
NC_000004.12 |
4 |
7309937 |
- |
SORCS2 |
NNNCTGAGCCCTGCCTACTG |
NGG |
2 |
0.1154 |
Tier II |
| 5 |
NC_000019.10 |
19 |
2271164 |
- |
OAZ1 |
NNNCTGAGCCCCGACTCCTC |
NGG |
2 |
0.0756 |
Tier II |
| 6 |
NC_000003.12 |
3 |
52452279 |
- |
TNNC1 |
NNNCTGAGCCCTGACTACTG |
NGC |
2 |
0.012 |
Tier II |
| 7 |
NC_000002.12 |
2 |
18676725 |
+ |
LOC105373456 |
NNNCTGAGACCAGACTACTG |
NGG |
2 |
0.6122 |
Tier III |
| 8 |
NC_000011.10 |
11 |
23520435 |
- |
RPS2P38 |
NNNCTCAGCCCCGACCACTG |
NGG |
2 |
0.4545 |
Tier III |
| 9 |
NC_000008.11 |
8 |
46851204 |
+ |
LINC00293 |
NNNCTGAGCCCTGCCTACTG |
NGG |
2 |
0.1154 |
Tier III |
| 10 |
NC_000024.10 |
Y |
22964150 |
+ |
TTTY4 |
NNNCTGAGCCCTGCCTACTG |
NGG |
2 |
0.1154 |
Tier III |
| 11 |
NC_000024.10 |
Y |
24597891 |
+ |
TTTY4B |
NNNCTGAGCCCTGCCTACTG |
NGG |
2 |
0.1154 |
Tier III |
| 12 |
NC_000024.10 |
Y |
25072200 |
- |
TTTY4C |
NNNCTGAGCCCTGCCTACTG |
NGG |
2 |
0.1154 |
Tier III |
| 13 |
NC_000011.10 |
11 |
76314141 |
- |
LOC105369395 |
NNNCTGAGCCCCGCCTACCG |
NGG |
2 |
0.0591 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
61083107 |
+ |
Grk5 |
NNNCTGAGCCCTGACTACTG |
NGG |
1 |
0.5385 |
Tier I |
| 2 |
NC_000085.6 |
19 |
61083107 |
+ |
Zfp950 |
NNNCTGAGCCCTGACTACTG |
NGG |
1 |
0.5385 |
Tier II |
| 3 |
NC_000075.6 |
9 |
111251980 |
+ |
Mlh1 |
NNNCTGAGCTCTGACTACTG |
NGG |
2 |
0.5068 |
Tier II |
| 4 |
NC_000072.6 |
6 |
86891055 |
- |
Aak1 |
NNNCTGAGTCCTGACTACTG |
NGG |
2 |
0.4712 |
Tier II |
| 5 |
NC_000075.6 |
9 |
99067563 |
+ |
Pik3cb |
NNNCTGAGCCCAGTCTACTG |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000071.6 |
5 |
34458522 |
- |
Fam193a |
NNNCTGAGCCTCGACAACTG |
NGG |
2 |
0.2797 |
Tier II |
| 7 |
NC_000068.7 |
2 |
166848042 |
- |
Arfgef2 |
NNNCTGTGCCCTGACTACTG |
NGG |
2 |
0.2356 |
Tier II |
| 8 |
NC_000067.6 |
1 |
192413266 |
- |
Kcnh1 |
NNNCTGAGCCCAGCCTACTG |
NGG |
2 |
0.1531 |
Tier II |
| 9 |
NC_000084.6 |
18 |
47024418 |
- |
Commd10 |
NNNCTGAGCCCTGACTGCTG |
NGG |
2 |
0.095 |
Tier II |
| 10 |
NC_000072.6 |
6 |
92424316 |
- |
Prickle2 |
NNNCTGAGCCGCTACTACTG |
NGG |
2 |
0.075 |
Tier II |
| 11 |
NC_000068.7 |
2 |
11252652 |
- |
Prkcq |
NNNCTGAGCCCTGACTAGTG |
NGG |
2 |
0.0718 |
Tier II |
| 12 |
NC_000070.6 |
4 |
53152588 |
+ |
Abca1 |
NNNCTGAGCCCAGACTACTG |
NGA |
2 |
0.0496 |
Tier II |
| 13 |
NC_000073.6 |
7 |
138229225 |
+ |
Tcerg1l |
NNNCTGAGCCCCGCCTGCTG |
NGG |
2 |
0.0378 |
Tier II |
| 14 |
NC_000077.6 |
11 |
4211901 |
+ |
Tbc1d10a |
NNNCTGAGCCCTGACTACTG |
NGA |
2 |
0.0374 |
Tier II |
| 15 |
NC_000072.6 |
6 |
125468597 |
+ |
Cd9 |
NNNCTGAGCCCCGACTAGCG |
NGG |
2 |
0.0368 |
Tier II |
| 16 |
NC_000072.6 |
6 |
92424316 |
- |
Gm38862 |
NNNCTGAGCCGCTACTACTG |
NGG |
2 |
0.075 |
Tier III |
| 17 |
NC_000073.6 |
7 |
138229225 |
+ |
Gm51503 |
NNNCTGAGCCCCGCCTGCTG |
NGG |
2 |
0.0378 |
Tier III |
| 18 |
NC_000080.6 |
14 |
21994179 |
+ |
A330001L22Rik |
NNNCTGAGCCCCGACTTCTG |
NAG |
2 |
0.0346 |
Tier III |
Other clones with same target sequence:
(none)
