Construct: sgRNA BRDN0001149167
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATGTTGCAGTTATCACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CERKL (375298)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77677
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
181549665 |
+ |
CERKL |
NNNGTTGCAGTTATCACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
113596828 |
+ |
TFDP1 |
NNNGTGGCATTTATCACATG |
NGG |
2 |
0.4643 |
Tier II |
| 3 |
NC_000001.11 |
1 |
17354389 |
- |
PADI4 |
NNNGGTGCTGTTATCACATG |
NGG |
2 |
0.384 |
Tier II |
| 4 |
NC_000007.14 |
7 |
110735044 |
- |
IMMP2L |
NNNGTGTCAGTTATCACATG |
NGG |
2 |
0.3265 |
Tier II |
| 5 |
NC_000016.10 |
16 |
81711041 |
- |
CMIP |
NNNGTTGCAGTGGTCACATG |
NGG |
2 |
0.3261 |
Tier II |
| 6 |
NC_000011.10 |
11 |
2428212 |
+ |
TRPM5 |
NNNGTTGCAGGTAACACATG |
NGG |
2 |
0.2476 |
Tier II |
| 7 |
NC_000021.9 |
21 |
45703898 |
+ |
PCBP3 |
NNNTTTGCAGTTGTCACATG |
NGG |
2 |
0.2372 |
Tier II |
| 8 |
NC_000004.12 |
4 |
120716061 |
+ |
PRDM5 |
NNNTTTGCAGTTATCACAAG |
NGG |
2 |
0.1039 |
Tier II |
| 9 |
NC_000001.11 |
1 |
171647827 |
+ |
MYOC |
NNNCTTGCAGTTATCACCTG |
NGG |
2 |
0.1008 |
Tier II |
| 10 |
NC_000013.11 |
13 |
100841786 |
+ |
NALCN-AS1 |
NNNGTTCCATTTATCACATG |
NGG |
2 |
0.5586 |
Tier III |
| 11 |
NC_000011.10 |
11 |
122057928 |
- |
MIR100HG |
NNNGTTTCAGTAATCACATG |
NGG |
2 |
0.4571 |
Tier III |
| 12 |
NC_000011.10 |
11 |
105298366 |
+ |
LOC105369468 |
NNNGTTGGAGCTATCACATG |
NGG |
2 |
0.4286 |
Tier III |
| 13 |
NC_000008.11 |
8 |
16378233 |
- |
LOC101929028 |
NNNGTTGCAGTGATAACATG |
NGG |
2 |
0.1111 |
Tier III |
| 14 |
NC_000006.12 |
6 |
160951044 |
- |
LOC102724087 |
NNNGTTGCAGATATTACATG |
NGG |
2 |
0.05 |
Tier III |
| 15 |
NC_000023.11 |
X |
136967147 |
+ |
TM9SF5P |
NNNGTTGCAGTTATCACATA |
NTG |
2 |
0.0365 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
144373458 |
+ |
Shank2 |
NNNGTTTCTGTTATCACATG |
NGG |
2 |
0.3429 |
Tier II |
| 2 |
NC_000081.6 |
15 |
91327555 |
- |
Slc2a13 |
NNNGTTGAAGTTATCACATG |
NAG |
2 |
0.1685 |
Tier II |
| 3 |
NC_000080.6 |
14 |
52326473 |
- |
Sall2 |
NNNATTGCAGTTATCACATG |
NGA |
2 |
0.0625 |
Tier II |
| 4 |
NC_000071.6 |
5 |
143051979 |
- |
Rnf216 |
NNNGCTGCAGTTATCACATG |
NTG |
2 |
0.039 |
Tier II |
| 5 |
NC_000067.6 |
1 |
6639543 |
- |
St18 |
NNNGTTGCAGTTCTCACATG |
NGT |
2 |
0.0034 |
Tier II |
| 6 |
NC_000074.6 |
8 |
57844197 |
+ |
Galntl6 |
NNNGTTGCAGTTATTACATG |
NGC |
2 |
0.0015 |
Tier II |
| 7 |
NC_000067.6 |
1 |
156042037 |
+ |
Tor1aip2 |
NNNGTTGCAGTTATCACATG |
NCC |
2 |
0.0 |
Tier II |
| 8 |
NC_000081.6 |
15 |
55799483 |
- |
Sntb1 |
NNNGTTTCAGTTAGCACATG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000067.6 |
1 |
143340775 |
+ |
Gm35244 |
NNNGTTGGAGTCATCACATG |
NGG |
2 |
0.609 |
Tier III |
| 10 |
NC_000073.6 |
7 |
144373458 |
+ |
Gm14372 |
NNNGTTTCTGTTATCACATG |
NGG |
2 |
0.3429 |
Tier III |
| 11 |
NC_000084.6 |
18 |
21480925 |
+ |
Gm31646 |
NNNGTTGAAGTTATTACATG |
NGG |
2 |
0.0433 |
Tier III |
| 12 |
NC_000071.6 |
5 |
143051979 |
- |
Gm43380 |
NNNGCTGCAGTTATCACATG |
NTG |
2 |
0.039 |
Tier III |
| 13 |
NC_000079.6 |
13 |
59203708 |
+ |
Gm34354 |
NNNGTTGCCGTTATGACATG |
NGG |
2 |
0.0286 |
Tier III |
| 14 |
NC_000079.6 |
13 |
59203708 |
+ |
Gm34558 |
NNNGTTGCCGTTATGACATG |
NGG |
2 |
0.0286 |
Tier III |
Other clones with same target sequence:
(none)
