Construct: sgRNA BRDN0001149172
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGGCAGTACCGATAAGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK1 (7443)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75525
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
96855250 |
- |
VRK1 |
NNNGGCAGTACCGATAAGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
6236532 |
+ |
MLLT1 |
NNNGGCAGCACCGAAAAGCA |
NGG |
2 |
0.5344 |
Tier II |
| 3 |
NC_000006.12 |
6 |
44883946 |
+ |
SUPT3H |
NNNGGCAATACCCATAAGCA |
NGG |
2 |
0.4211 |
Tier II |
| 4 |
NC_000004.12 |
4 |
86123607 |
+ |
MAPK10 |
NNNGGCACTACCGAAAAGCA |
NGG |
2 |
0.3563 |
Tier II |
| 5 |
NC_000007.14 |
7 |
133821683 |
- |
EXOC4 |
NNNGGCAGTACCGAAAAGTA |
NGG |
2 |
0.2672 |
Tier II |
| 6 |
NC_000002.12 |
2 |
109152258 |
+ |
RANBP2 |
NNNGGCAGTACCTATAATCA |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000002.12 |
2 |
109152258 |
+ |
SH3RF3 |
NNNGGCAGTACCTATAATCA |
NGG |
2 |
0.2 |
Tier II |
| 8 |
NC_000004.12 |
4 |
86123607 |
+ |
MAPK10-AS1 |
NNNGGCACTACCGAAAAGCA |
NGG |
2 |
0.3563 |
Tier III |
| 9 |
NC_000007.14 |
7 |
133821683 |
- |
LOC101928861 |
NNNGGCAGTACCGAAAAGTA |
NGG |
2 |
0.2672 |
Tier III |
| 10 |
NC_000005.10 |
5 |
129341798 |
+ |
LOC102723654 |
NNNGGCAGTGCCTATAAGCA |
NGG |
2 |
0.1667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
106057869 |
- |
Vrk1 |
NNNGGCAGTACCGATAAGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
101910111 |
+ |
Lamtor1 |
NNNGGCAGTACAGGTAAGCA |
NGG |
2 |
0.3333 |
Tier I |
| 3 |
NC_000086.7 |
X |
73504210 |
+ |
Atp2b3 |
NNNGGCAGGACAGATAAGCA |
NGG |
2 |
0.4422 |
Tier II |
| 4 |
NC_000067.6 |
1 |
151424293 |
- |
Swt1 |
NNNGGCACTACAGATAAGCA |
NGG |
2 |
0.4396 |
Tier II |
| 5 |
NC_000083.6 |
17 |
27620612 |
+ |
Nudt3 |
NNNAGCAGTACCCATAAGCA |
NGG |
2 |
0.3789 |
Tier II |
| 6 |
NC_000072.6 |
6 |
121880387 |
- |
Mug1 |
NNNGGCAGTACCTATAAACA |
NGG |
2 |
0.2077 |
Tier II |
| 7 |
NC_000085.6 |
19 |
48284179 |
- |
Sorcs3 |
NNNGGCAGTACCGAGAACCA |
NGG |
2 |
0.0238 |
Tier II |
| 8 |
NC_000084.6 |
18 |
25700064 |
+ |
Celf4 |
NNNGGCAGTACCTATAAGCA |
NGC |
2 |
0.0067 |
Tier II |
Other clones with same target sequence:
(none)
