Construct: sgRNA BRDN0001149184
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGGTCATCCATGCCTGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNIK (23043)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75852
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000003.12 |
3 |
171211138 |
+ |
TNIK |
NNNGGTCATCCATGCCTGGT |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000009.12 |
9 |
134820130 |
+ |
COL5A1 |
NNNGGTCATCCAGGCCTGAT |
NGG |
2 |
0.1863 |
Tier I |
3 |
NC_000008.11 |
8 |
2966657 |
- |
CSMD1 |
NNNGGTCATCTATGCCTGCT |
NGG |
2 |
0.1379 |
Tier I |
4 |
NC_000016.10 |
16 |
88723319 |
+ |
PIEZO1 |
NNNGGTCACCCATGCCTGGT |
NCG |
2 |
0.0989 |
Tier I |
5 |
NC_000017.11 |
17 |
58534624 |
- |
SEPTIN4 |
NNNGGCCATCAATGCCTGGT |
NGG |
2 |
0.6818 |
Tier II |
6 |
NC_000019.10 |
19 |
41343668 |
+ |
TGFB1 |
NNNGGTCATTCCTGCCTGGT |
NGG |
2 |
0.2477 |
Tier II |
7 |
NC_000003.12 |
3 |
38004421 |
+ |
VILL |
NNNGGCCATCCCTGCCTGGT |
NGG |
2 |
0.2392 |
Tier II |
8 |
NC_000007.14 |
7 |
157400486 |
+ |
DNAJB6 |
NNNGGTCATCCTTCCCTGGT |
NGG |
2 |
0.1429 |
Tier II |
9 |
NC_000012.12 |
12 |
82791848 |
- |
TMTC2 |
NNNGGTCCTCCATGCCTGGT |
NAG |
2 |
0.1111 |
Tier II |
10 |
NC_000002.12 |
2 |
105303527 |
- |
TGFBRAP1 |
NNNTTTCATCCATGCCTGGT |
NGG |
2 |
0.1091 |
Tier II |
11 |
NC_000006.12 |
6 |
79559417 |
- |
SH3BGRL2 |
NNNGGTTATCCATGCCTGGC |
NGG |
2 |
0.0739 |
Tier II |
12 |
NC_000022.11 |
22 |
32545179 |
- |
SYN3 |
NNNGATCATCCATGCCTGGT |
NTG |
2 |
0.0338 |
Tier II |
13 |
NC_000008.11 |
8 |
143976705 |
+ |
PLEC |
NNNGGTCATACATGCCTGGT |
NGC |
2 |
0.0193 |
Tier II |
14 |
NC_000022.11 |
22 |
47113413 |
+ |
TBC1D22A |
NNNGGTCAGCCATGCCTGGT |
NGT |
2 |
0.01 |
Tier II |
15 |
NC_000017.11 |
17 |
58534624 |
- |
SEPTIN4-AS1 |
NNNGGCCATCAATGCCTGGT |
NGG |
2 |
0.6818 |
Tier III |
16 |
NC_000010.11 |
10 |
112313555 |
- |
GUCY2GP |
NNNGCTCATACATGCCTGGT |
NGG |
2 |
0.681 |
Tier III |
17 |
NC_000009.12 |
9 |
134820130 |
+ |
LOC101448202 |
NNNGGTCATCCAGGCCTGAT |
NGG |
2 |
0.1863 |
Tier III |
18 |
NC_000008.11 |
8 |
2966657 |
- |
LOC105377785 |
NNNGGTCATCTATGCCTGCT |
NGG |
2 |
0.1379 |
Tier III |
19 |
NC_000001.11 |
1 |
1168595 |
- |
LOC124903818 |
NNNGGTCATCCTGGCCTGGT |
NGG |
2 |
0.087 |
Tier III |
20 |
NC_000022.11 |
22 |
32545179 |
- |
LOC124905103 |
NNNGATCATCCATGCCTGGT |
NTG |
2 |
0.0338 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000073.6 |
7 |
29244101 |
- |
Yif1b |
NNNGATCAGCCATGCCTGGT |
NGG |
2 |
0.5365 |
Tier I |
2 |
NC_000069.6 |
3 |
28505857 |
- |
Tnik |
NNNGGTCATCCATGCCAGGA |
NGG |
2 |
0.3 |
Tier I |
3 |
NC_000083.6 |
17 |
17844169 |
+ |
Has1 |
NNNGGTCATCCATGCGTGGT |
NGC |
2 |
0.0034 |
Tier I |
4 |
NC_000072.6 |
6 |
53500672 |
+ |
Creb5 |
NNNGGCCATCCATCCCTGGT |
NGG |
2 |
0.3896 |
Tier II |
5 |
NC_000084.6 |
18 |
57247961 |
+ |
Megf10 |
NNNGCTCTTCCATGCCTGGT |
NGG |
2 |
0.3367 |
Tier II |
6 |
NC_000068.7 |
2 |
59138740 |
- |
Ccdc148 |
NNNGGTCATCCCTGCCTGTT |
NGG |
2 |
0.1754 |
Tier II |
7 |
NC_000083.6 |
17 |
32136292 |
+ |
Notch3 |
NNNTGTCATGCATGCCTGGT |
NGG |
2 |
0.1414 |
Tier II |
8 |
NC_000072.6 |
6 |
51534448 |
+ |
Snx10 |
NNNGGTCATCCATGCCTGTG |
NGG |
2 |
0.1176 |
Tier II |
9 |
NC_000079.6 |
13 |
41262348 |
- |
Smim13 |
NNNGGTCATCCATGCCTGTT |
NTG |
2 |
0.026 |
Tier II |
10 |
NC_000073.6 |
7 |
116227946 |
- |
Plekha7 |
NNNGGTCATCCATGCCGGGG |
NGG |
2 |
0.0208 |
Tier II |
11 |
NC_000076.6 |
10 |
107680409 |
+ |
Ptprq |
NNNGGACATCCATGCCTGGT |
NGT |
2 |
0.014 |
Tier II |
12 |
NC_000079.6 |
13 |
107797385 |
- |
Zswim6 |
NNNGGGCATCCATGCCTGGT |
NGT |
2 |
0.0092 |
Tier II |
13 |
NC_000070.6 |
4 |
150854518 |
+ |
Errfi1 |
NNNGGTCATCCATCCCTGGT |
NGT |
2 |
0.0069 |
Tier II |
14 |
NC_000076.6 |
10 |
43073220 |
- |
Sobp |
NNNGGTCATCCATGCCTGGG |
NGT |
2 |
0.0028 |
Tier II |
15 |
NC_000068.7 |
2 |
10330171 |
+ |
Gm13261 |
NNNGGTGATACATGCCTGGT |
NGG |
2 |
0.4078 |
Tier III |
16 |
NC_000073.6 |
7 |
31817609 |
+ |
Gm6669 |
NNNGGTCATCCATGACAGGT |
NGG |
2 |
0.1185 |
Tier III |
17 |
NC_000073.6 |
7 |
32546258 |
+ |
Gm5327 |
NNNGGTCATCCATGACAGGT |
NGG |
2 |
0.1185 |
Tier III |
18 |
NC_000078.6 |
12 |
113409980 |
- |
Igh |
NNNGGTCTTCCATGCCTGGT |
NGA |
2 |
0.0298 |
Tier III |
19 |
NC_000078.6 |
12 |
113409980 |
- |
Ighd |
NNNGGTCTTCCATGCCTGGT |
NGA |
2 |
0.0298 |
Tier III |
20 |
NC_000070.6 |
4 |
150854518 |
+ |
1700045H11Rik |
NNNGGTCATCCATCCCTGGT |
NGT |
2 |
0.0069 |
Tier III |
Other clones with same target sequence:
(none)