Construct: sgRNA BRDN0001149184

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTGGTCATCCATGCCTGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNIK (23043)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75852

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 171211138 + TNIK NNNGGTCATCCATGCCTGGT NGG 0 1.0 Tier I
2 NC_000009.12 9 134820130 + COL5A1 NNNGGTCATCCAGGCCTGAT NGG 2 0.1863 Tier I
3 NC_000008.11 8 2966657 - CSMD1 NNNGGTCATCTATGCCTGCT NGG 2 0.1379 Tier I
4 NC_000016.10 16 88723319 + PIEZO1 NNNGGTCACCCATGCCTGGT NCG 2 0.0989 Tier I
5 NC_000017.11 17 58534624 - SEPTIN4 NNNGGCCATCAATGCCTGGT NGG 2 0.6818 Tier II
6 NC_000019.10 19 41343668 + TGFB1 NNNGGTCATTCCTGCCTGGT NGG 2 0.2477 Tier II
7 NC_000003.12 3 38004421 + VILL NNNGGCCATCCCTGCCTGGT NGG 2 0.2392 Tier II
8 NC_000007.14 7 157400486 + DNAJB6 NNNGGTCATCCTTCCCTGGT NGG 2 0.1429 Tier II
9 NC_000012.12 12 82791848 - TMTC2 NNNGGTCCTCCATGCCTGGT NAG 2 0.1111 Tier II
10 NC_000002.12 2 105303527 - TGFBRAP1 NNNTTTCATCCATGCCTGGT NGG 2 0.1091 Tier II
11 NC_000006.12 6 79559417 - SH3BGRL2 NNNGGTTATCCATGCCTGGC NGG 2 0.0739 Tier II
12 NC_000022.11 22 32545179 - SYN3 NNNGATCATCCATGCCTGGT NTG 2 0.0338 Tier II
13 NC_000008.11 8 143976705 + PLEC NNNGGTCATACATGCCTGGT NGC 2 0.0193 Tier II
14 NC_000022.11 22 47113413 + TBC1D22A NNNGGTCAGCCATGCCTGGT NGT 2 0.01 Tier II
15 NC_000017.11 17 58534624 - SEPTIN4-AS1 NNNGGCCATCAATGCCTGGT NGG 2 0.6818 Tier III
16 NC_000010.11 10 112313555 - GUCY2GP NNNGCTCATACATGCCTGGT NGG 2 0.681 Tier III
17 NC_000009.12 9 134820130 + LOC101448202 NNNGGTCATCCAGGCCTGAT NGG 2 0.1863 Tier III
18 NC_000008.11 8 2966657 - LOC105377785 NNNGGTCATCTATGCCTGCT NGG 2 0.1379 Tier III
19 NC_000001.11 1 1168595 - MIR200BHG NNNGGTCATCCTGGCCTGGT NGG 2 0.087 Tier III
20 NC_000022.11 22 32545179 - LOC124905103 NNNGATCATCCATGCCTGGT NTG 2 0.0338 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 29244101 - Yif1b NNNGATCAGCCATGCCTGGT NGG 2 0.5365 Tier I
2 NC_000069.6 3 28505857 - Tnik NNNGGTCATCCATGCCAGGA NGG 2 0.3 Tier I
3 NC_000083.6 17 17844169 + Has1 NNNGGTCATCCATGCGTGGT NGC 2 0.0034 Tier I
4 NC_000072.6 6 53500672 + Creb5 NNNGGCCATCCATCCCTGGT NGG 2 0.3896 Tier II
5 NC_000084.6 18 57247961 + Megf10 NNNGCTCTTCCATGCCTGGT NGG 2 0.3367 Tier II
6 NC_000068.7 2 59138740 - Ccdc148 NNNGGTCATCCCTGCCTGTT NGG 2 0.1754 Tier II
7 NC_000083.6 17 32136292 + Notch3 NNNTGTCATGCATGCCTGGT NGG 2 0.1414 Tier II
8 NC_000072.6 6 51534448 + Snx10 NNNGGTCATCCATGCCTGTG NGG 2 0.1176 Tier II
9 NC_000079.6 13 41262348 - Smim13 NNNGGTCATCCATGCCTGTT NTG 2 0.026 Tier II
10 NC_000073.6 7 116227946 - Plekha7 NNNGGTCATCCATGCCGGGG NGG 2 0.0208 Tier II
11 NC_000076.6 10 107680409 + Ptprq NNNGGACATCCATGCCTGGT NGT 2 0.014 Tier II
12 NC_000079.6 13 107797385 - Zswim6 NNNGGGCATCCATGCCTGGT NGT 2 0.0092 Tier II
13 NC_000070.6 4 150854518 + Errfi1 NNNGGTCATCCATCCCTGGT NGT 2 0.0069 Tier II
14 NC_000076.6 10 43073220 - Sobp NNNGGTCATCCATGCCTGGG NGT 2 0.0028 Tier II
15 NC_000068.7 2 10330171 + Gm13261 NNNGGTGATACATGCCTGGT NGG 2 0.4078 Tier III
16 NC_000073.6 7 31817609 + Gm6669 NNNGGTCATCCATGACAGGT NGG 2 0.1185 Tier III
17 NC_000073.6 7 32546258 + Gm5327 NNNGGTCATCCATGACAGGT NGG 2 0.1185 Tier III
18 NC_000078.6 12 113409980 - Igh NNNGGTCTTCCATGCCTGGT NGA 2 0.0298 Tier III
19 NC_000078.6 12 113409980 - Ighd NNNGGTCTTCCATGCCTGGT NGA 2 0.0298 Tier III
20 NC_000070.6 4 150854518 + 1700045H11Rik NNNGGTCATCCATCCCTGGT NGT 2 0.0069 Tier III
Download CSV

Other clones with same target sequence:

(none)

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