Construct: sgRNA BRDN0001149190

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTCTGGGCCCACCCAACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK2 (9344)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77251

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 29986570 + TAOK2 NNNCTGGGCCCACCCAACAC NGG 0 1.0 Tier I
2 NC_000016.10 16 568071 - NHLRC4 NNNCTGGGCCCACCCAGCAG NGG 2 0.0104 Tier I
3 NC_000007.14 7 96298133 + SLC25A13 NNNCTGGAACCACCCAACAC NGG 2 0.8571 Tier II
4 NC_000008.11 8 142663871 + JRK NNNCGGGGTCCACCCAACAC NGG 2 0.56 Tier II
5 NC_000016.10 16 46894269 - GPT2 NNNCTGGGCCAGCCCAACAC NGG 2 0.5417 Tier II
6 NC_000008.11 8 51665508 + PXDNL NNNCTGTTCCCACCCAACAC NGG 2 0.3571 Tier II
7 NC_000001.11 1 33133752 + AZIN2 NNNCTTGGCCCACACAACAC NGG 2 0.2333 Tier II
8 NC_000001.11 1 33133752 + ZNF362 NNNCTTGGCCCACACAACAC NGG 2 0.2333 Tier II
9 NC_000010.11 10 103544296 - NEURL1 NNNCTGGCCCCACCCAACAT NGG 2 0.1846 Tier II
10 NC_000011.10 11 61785043 - MYRF NNNCTAGGCCCACCCAGCAC NGG 2 0.1765 Tier II
11 NC_000010.11 10 98611090 - HPSE2 NNNCTGGCCCCCCCCAACAC NGG 2 0.1619 Tier II
12 NC_000015.10 15 92466921 - ST8SIA2 NNNCTGGGGCCACCCAACAC NAG 2 0.1605 Tier II
13 NC_000011.10 11 44270795 + ALX4 NNNCTGGGCCCACTCAGCAC NGG 2 0.1294 Tier II
14 NC_000001.11 1 6586818 - ZBTB48 NNNCTGGGGCCACCCAACCC NGG 2 0.1281 Tier II
15 NC_000002.12 2 152024036 - CACNB4 NNNCTGTGCCCAGCCAACAC NGG 2 0.0779 Tier II
16 NC_000012.12 12 104926800 - SLC41A2 NNNCTGTGCCCAGCCAACAC NGG 2 0.0779 Tier II
17 NC_000006.12 6 39310530 - KCNK17 NNNCTGAGCCCACCCAACAC NGA 2 0.0694 Tier II
18 NC_000014.9 14 104794937 + AKT1 NNNCAGGGCCCACCCAAGAC NGG 2 0.0667 Tier II
19 NC_000022.11 22 46102695 + LOC124905135 NNNCTGGGCTCACCCAACAC NGA 2 0.0654 Tier II
20 NC_000002.12 2 239330489 + HDAC4 NNNCTGGCCCCACCGAACAC NGG 2 0.0308 Tier II
21 NC_000001.11 1 110598571 + KCNA2 NNNCTGGGCCCTCCCAACAC NGA 2 0.0231 Tier II
22 NC_000010.11 10 102327864 - GBF1 NNNCTGGGCCCAGCCAACAC NTG 2 0.0053 Tier II
23 NC_000007.14 7 50130494 + SPMIP7 NNNCTGGCCCCACCCTACAC NGG 2 0.0 Tier II
24 NC_000017.11 17 66837440 + CACNG5 NNNCTGGGCCCACCCCACAC NGC 2 0.0 Tier II
25 NC_000001.11 1 226990316 - CDC42BPA NNNCTGGGCCCTCCCCACAC NGG 2 0.0 Tier II
26 NC_000020.11 20 63026333 + LOC105372718 NNNCTCGGACCACCCAACAC NGG 2 0.5844 Tier III
27 NC_000016.10 16 2610350 + LOC652276 NNNCTGGGCCCACCCAATAC NAG 2 0.1667 Tier III
28 NC_000007.14 7 130338444 - LOC105375503 NNNCTGGGGCCACCCACCAC NGG 2 0.1092 Tier III
29 NC_000008.11 8 16752698 + LOC101929028 NNNCTGTGCCCAGCCAACAC NGG 2 0.0779 Tier III
30 NC_000022.11 22 46102695 + MIRLET7BHG NNNCTGGGCTCACCCAACAC NGA 2 0.0654 Tier III
31 NC_000004.12 4 99403478 - LOC102723576 NNNCTGGGCCCCCCCAACAG NGG 2 0.0155 Tier III
32 NC_000001.11 1 189457 - WASH9P NNNCTGGGCCCACCCCACAC NAG 2 0.0 Tier III
33 NC_000001.11 1 38394045 - LOC105378657 NNNCTGGGTCCACCCCACAC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 6404183 + Zmiz2 NNNCTGGGCCAACCAAACAC NGG 2 0.1667 Tier I
2 NC_000070.6 4 116173470 - 1700042G07Rik NNNCTGGGCCCACCCATCCC NGG 2 0.0276 Tier I
3 NC_000068.7 2 130422784 + Pced1a NNNCTGCACCCACCCAACAC NGG 2 0.6875 Tier II
4 NC_000078.6 12 79784032 - Rad51b NNNCTGGGTCAACCCAACAC NGG 2 0.6563 Tier II
5 NC_000082.6 16 91820328 - Itsn1 NNNCAGGGCTCACCCAACAC NGG 2 0.4706 Tier II
6 NC_000077.6 11 97568128 + Srcin1 NNNCTGGGACCACCCAACTC NGG 2 0.4615 Tier II
7 NC_000071.6 5 8493907 + Rundc3b NNNCTGGGCCCACACAACAC NGG 1 0.35 Tier II
8 NC_000071.6 5 38354334 + Slc2a9 NNNGTGGCCCCACCCAACAC NGG 2 0.3077 Tier II
9 NC_000084.6 18 13914701 + Zfp521 NNNCTGGGCCAAACCAACAC NGG 2 0.2885 Tier II
10 NC_000067.6 1 39943812 - Map4k4 NNNCTAGGCCCACCCAACAC NAG 2 0.2593 Tier II
11 NC_000070.6 4 140600123 + Arhgef10l NNNCTGAGCCCACCCAACAC NAG 2 0.2593 Tier II
12 NC_000080.6 14 35581346 - Grid1 NNNATGGGCCCACCCAACAT NGG 2 0.2526 Tier II
13 NC_000083.6 17 44665363 + Runx2 NNNCTGGGTCCCCCCAACAC NGG 2 0.2303 Tier II
14 NC_000067.6 1 143759853 + Ro60 NNNCTGAGCCCACCCGACAC NGG 2 0.1923 Tier II
15 NC_000085.6 19 12651657 - Glyat NNNCTGGGCCCACTCAACAC NAG 2 0.1901 Tier II
16 NC_000074.6 8 3386498 + Arhgef18 NNNCTGGGACCACCCACCAC NGG 2 0.1513 Tier II
17 NC_000081.6 15 43099736 + Rspo2 NNNCTAGGCCCACCCAAGAC NGG 2 0.1333 Tier II
18 NC_000073.6 7 29343948 + Sipa1l3 NNNCTGGGGCCAGCCAACAC NGG 2 0.0844 Tier II
19 NC_000068.7 2 92379667 + Pex16 NNNCTGGGCCCACCCAACAC NGA 1 0.0694 Tier II
20 NC_000070.6 4 44568885 - Pax5 NNNCTGGGCCCTGCCAACAC NGG 2 0.0455 Tier II
21 NC_000068.7 2 169831885 - Tshz2 NNNCTGGGGCCACCCAACAC NGA 2 0.043 Tier II
22 NC_000077.6 11 62859773 - Fbxw10 NNNCTGGGCCCACACAACAC NTG 2 0.0136 Tier II
23 NC_000071.6 5 150200941 - Fry NNNCTGGGCCCACCCAGCAG NGG 2 0.0104 Tier II
24 NC_000070.6 4 8725086 - Chd7 NNNCTGGACCCACCCCACAC NGG 2 0.0 Tier II
25 NC_000082.6 16 11542877 - Snx29 NNNCTGGGACCACCCTACAC NGG 2 0.0 Tier II
26 NC_000068.7 2 164185327 + A730032A03Rik NNNCTGGACACACCCAACAC NGG 2 0.8667 Tier III
27 NC_000076.6 10 41091648 - Gm32284 NNNCTGGGCCCAGCCAGCAC NGG 2 0.0241 Tier III
28 NC_000070.6 4 8725086 - Gm46841 NNNCTGGACCCACCCCACAC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)