Construct: sgRNA BRDN0001149198

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTATTACTGATATTGGTGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ONE_INTERGENIC_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80248

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 10285835 - SBF2 NNNTTACTGTTATTGGTGGA NGG 2 0.8272 Tier II
2 NC_000002.12 2 101755471 + MAP4K4 NNNTTACTGAAATTGGTGGG NGG 1 0.75 Tier II
3 NC_000002.12 2 196339173 + HECW2 NNNTTTCTGATATTGGTGGG NGG 1 0.7143 Tier II
4 NC_000019.10 19 56607950 + ZNF71 NNNTTACTGACATTGGTGGG NGG 1 0.6667 Tier II
5 NC_000001.11 1 183599910 - NCF2 NNNTTACTGATATTGGTAGA NGG 2 0.649 Tier II
6 NC_000010.11 10 11201337 + CELF2 NNNTTATGGATATTGGTGGG NGG 2 0.5958 Tier II
7 NC_000011.10 11 124673073 - SIAE NNNTTACAGATATTGGTGGT NGG 2 0.56 Tier II
8 NC_000007.14 7 32033912 - PDE1C NNNTTACTGATAATGGTTGG NGG 2 0.4615 Tier II
9 NC_000023.11 X 143033551 + SPANXN4 NNNTTACAGGTATTGGTGGG NGG 2 0.4444 Tier II
10 NC_000018.10 18 36458101 - FHOD3 NNNTTTCTGATATAGGTGGG NGG 2 0.4422 Tier II
11 NC_000001.11 1 175706085 - TNR NNNTTATTGATTTTGGTGGG NGG 2 0.2708 Tier II
12 NC_000002.12 2 239971544 + NDUFA10 NNNTTACTGATATTAGTGGG NAG 2 0.244 Tier II
13 NC_000002.12 2 32235101 + NLRC4 NNNTTCCTGATATTGGAGGG NGG 2 0.2424 Tier II
14 NC_000012.12 12 920829 - RAD52 NNNTTTCTGCTATTGGTGGG NGG 2 0.2381 Tier II
15 NC_000002.12 2 86504329 + CHMP3 NNNATACTGATAGTGGTGGG NGG 2 0.1242 Tier II
16 NC_000002.12 2 86504329 + RNF103-CHMP3 NNNATACTGATAGTGGTGGG NGG 2 0.1242 Tier II
17 NC_000009.12 9 8576375 + PTPRD NNNTTACTGATATTTGTGGG NAG 2 0.037 Tier II
18 NC_000006.12 6 16534669 - ATXN1 NNNTTACTGATCTTGGTGGG NTG 2 0.0103 Tier II
19 NC_000004.12 4 39467866 - LIAS NNNTAACTGATATTGCTGGG NGG 2 0.0 Tier II
20 NC_000001.11 1 165631423 - MGST3 NNNTTACTGATAAGGGTGGG NGG 2 0.0 Tier II
21 NC_000011.10 11 99745326 - CNTN5 NNNTTACTGATATTTCTGGG NGG 2 0.0 Tier II
22 NC_000019.10 19 56607950 + ZNF71-SMIM17 NNNTTACTGACATTGGTGGG NGG 1 0.6667 Tier III
23 NC_000010.11 10 11201337 + LOC124902375 NNNTTATGGATATTGGTGGG NGG 2 0.5958 Tier III
24 NC_000006.12 6 167462737 - LOC105378127 NNNTTACTCATATTGGTGGG NGG 1 0.5385 Tier III
25 NC_000010.11 10 70997369 + LOC105378350 NNNCTTCTGATATTGGTGGG NGG 2 0.4622 Tier III
26 NC_000002.12 2 8289031 - LINC00299 NNNTTACTGATATTGATGGG NAG 2 0.2593 Tier III
27 NC_000014.9 14 86113140 - LINC02328 NNNTTACTGAAATTGGTGGG NGA 2 0.0521 Tier III
28 NC_000001.11 1 113924661 - HIPK1-AS1 NNNTTACTGATATTGGTGAG NGC 2 0.0159 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 40754777 + Cc2d2b NNNTTAATGAAATTGGTGGG NGG 2 0.5625 Tier II
2 NC_000067.6 1 133176097 + Plekha6 NNNTTACTGAAATAGGTGGG NGG 2 0.4643 Tier II
3 NC_000068.7 2 163292681 - Tox2 NNNTTCCTGATATTGGAGGG NGG 2 0.2424 Tier II
4 NC_000071.6 5 74253035 + Scfd2 NNNATACTGCTATTGGTGGG NGG 2 0.1587 Tier II
5 NC_000070.6 4 98998866 - Dock7 NNNTTTCTGATATTGGGGGG NGG 2 0.084 Tier II
6 NC_000084.6 18 56392263 - Gramd3 NNNTTACTGATATTTGTGGG NAG 2 0.037 Tier II
7 NC_000078.6 12 5443061 + Gm32214 NNNTTGCTAATATTGGTGGG NGG 2 0.4592 Tier III
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Other clones with same target sequence:

(none)