Construct: sgRNA BRDN0001149211
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCTTGTCATTTGACAACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCE (5581)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76790
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
46007511 |
+ |
PRKCE |
NNNTTGTCATTTGACAACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
167486227 |
- |
CD247 |
NNNTTCTCATTTGACAACCG |
NGG |
1 |
0.6818 |
Tier II |
| 3 |
NC_000007.14 |
7 |
142156628 |
+ |
MGAM2 |
NNNTTGTCAGTTGACAACCA |
NGG |
2 |
0.4688 |
Tier II |
| 4 |
NC_000016.10 |
16 |
27772683 |
+ |
KATNIP |
NNNTTGTCATTTCACAACCA |
NGG |
2 |
0.3947 |
Tier II |
| 5 |
NC_000003.12 |
3 |
58404641 |
+ |
PXK |
NNNTTGTCATTAGACAACTG |
NGG |
2 |
0.3692 |
Tier II |
| 6 |
NC_000020.11 |
20 |
14643742 |
- |
MACROD2 |
NNNTTGTCATGTGACAATCG |
NGG |
2 |
0.2571 |
Tier II |
| 7 |
NC_000002.12 |
2 |
79322751 |
+ |
CTNNA2 |
NNNTTGTCATTTGACAAATG |
NGG |
2 |
0.2485 |
Tier II |
| 8 |
NC_000015.10 |
15 |
27157416 |
+ |
GABRG3 |
NNNTTGTCATTTGACAGCAG |
NGG |
2 |
0.0756 |
Tier II |
| 9 |
NC_000009.12 |
9 |
8332261 |
- |
PTPRD |
NNNTTGTCATTTGACAACGG |
NAG |
2 |
0.0324 |
Tier II |
| 10 |
NC_000023.11 |
X |
13570504 |
- |
EGFL6 |
NNNTTGTCATCTGACAACCG |
NGT |
2 |
0.0108 |
Tier II |
| 11 |
NC_000017.11 |
17 |
48602249 |
+ |
HOXB6 |
NNNTTGTCTTTTGACAACCG |
NGT |
2 |
0.0097 |
Tier II |
| 12 |
NC_000002.12 |
2 |
95965317 |
+ |
ANKRD36C |
NNNTTGTCATTTGACATCCG |
NGA |
2 |
0.0093 |
Tier II |
| 13 |
NC_000002.12 |
2 |
12151803 |
- |
MIR3681HG |
NNNTTGTCATTTGTCAACCT |
NGG |
2 |
0.3733 |
Tier III |
| 14 |
NC_000016.10 |
16 |
33748454 |
+ |
LOC102724207 |
NNNTTGTCATTTGACAACTG |
NGA |
2 |
0.0321 |
Tier III |
| 15 |
NC_000017.11 |
17 |
48602249 |
+ |
HOXB-AS3 |
NNNTTGTCTTTTGACAACCG |
NGT |
2 |
0.0097 |
Tier III |
| 16 |
NT_187383.1 |
16 |
1594710 |
+ |
LOC102723869 |
NNNTTGTCATTTGACATCCG |
NGA |
2 |
0.0093 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
86493270 |
+ |
Prkce |
NNNTTGTCATTTGACAACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
65714834 |
+ |
Map2k4 |
NNNTTGTCATTAGACAACTG |
NGG |
2 |
0.3692 |
Tier II |
| 3 |
NC_000081.6 |
15 |
27987914 |
- |
Trio |
NNNTGGTCATTTGACACCCG |
NGG |
2 |
0.1129 |
Tier II |
| 4 |
NC_000081.6 |
15 |
27987914 |
- |
Gm29975 |
NNNTGGTCATTTGACACCCG |
NGG |
2 |
0.1129 |
Tier II |
| 5 |
NC_000077.6 |
11 |
115837247 |
+ |
Llgl2 |
NNNTTGTCAGTTGACACCCG |
NGG |
2 |
0.0882 |
Tier II |
| 6 |
NC_000084.6 |
18 |
38989362 |
+ |
Arhgap26 |
NNNTTGTCACTTGATAACCG |
NGG |
2 |
0.0356 |
Tier II |
| 7 |
NC_000077.6 |
11 |
96295182 |
- |
Hoxb6 |
NNNTTGTCTTTTGACAACCG |
NGT |
2 |
0.0097 |
Tier II |
| 8 |
NC_000084.6 |
18 |
26845479 |
- |
Gm33621 |
NNNTTGTCACTTGACACCCG |
NGG |
2 |
0.0941 |
Tier III |
| 9 |
NC_000077.6 |
11 |
96295182 |
- |
Hoxb5os |
NNNTTGTCTTTTGACAACCG |
NGT |
2 |
0.0097 |
Tier III |
Other clones with same target sequence:
(none)
