Construct: sgRNA BRDN0001149216
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGCTCGAGTCATTTCTCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80229
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
41658404 |
- |
LIMCH1 |
NNNCTCAAGACATTTCTCTA |
NGG |
2 |
0.8571 |
Tier II |
| 2 |
NC_000010.11 |
10 |
86818821 |
- |
BMPR1A |
NNNCTTGAGTAATTTCTCTA |
NGG |
2 |
0.6964 |
Tier II |
| 3 |
NC_000004.12 |
4 |
142816526 |
+ |
INPP4B |
NNNTTCCAGTCATTTCTCTA |
NGG |
2 |
0.55 |
Tier II |
| 4 |
NC_000006.12 |
6 |
104823934 |
+ |
HACE1 |
NNNCTCCAGTCATTTCTCTG |
NGG |
2 |
0.5257 |
Tier II |
| 5 |
NC_000020.11 |
20 |
37355636 |
+ |
SRC |
NNNCTCCAGTCATTTCCCTA |
NGG |
2 |
0.4853 |
Tier II |
| 6 |
NC_000016.10 |
16 |
8742249 |
+ |
ABAT |
NNNCTCCAGTCATTTCTCTC |
NGG |
2 |
0.1563 |
Tier II |
| 7 |
NC_000023.11 |
X |
12676963 |
- |
FRMPD4 |
NNNCTGGAGTCATTTCTCAA |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000003.12 |
3 |
108200684 |
+ |
IFT57 |
NNNCTTGAGTCATTTCTGTA |
NGG |
2 |
0.1238 |
Tier II |
| 9 |
NC_000013.11 |
13 |
59918407 |
- |
DIAPH3 |
NNNCTCGAGTCTTTTCTCAA |
NGG |
2 |
0.0952 |
Tier II |
| 10 |
NC_000018.10 |
18 |
58749483 |
+ |
MALT1 |
NNNCTCCAGTCATTTCTCTA |
NGA |
2 |
0.0477 |
Tier II |
| 11 |
NC_000002.12 |
2 |
113175443 |
+ |
PSD4 |
NNNCTCGAGTCATTTCTCTC |
NCG |
2 |
0.0244 |
Tier II |
| 12 |
NC_000005.10 |
5 |
148510750 |
+ |
HTR4 |
NNNCTAGAGTCATTTCTCTA |
NGT |
2 |
0.015 |
Tier II |
| 13 |
NC_000004.12 |
4 |
142816526 |
+ |
USP38-DT |
NNNTTCCAGTCATTTCTCTA |
NGG |
2 |
0.55 |
Tier III |
| 14 |
NC_000004.12 |
4 |
127257125 |
- |
LOC102724210 |
NNNCTGCAGTCATTTCTCTA |
NGG |
2 |
0.3438 |
Tier III |
| 15 |
NC_000004.12 |
4 |
127257125 |
- |
LOC107986312 |
NNNCTGCAGTCATTTCTCTA |
NGG |
2 |
0.3438 |
Tier III |
| 16 |
NC_000018.10 |
18 |
58749483 |
+ |
LOC105372146 |
NNNCTCCAGTCATTTCTCTA |
NGA |
2 |
0.0477 |
Tier III |
| 17 |
NC_000002.12 |
2 |
113175443 |
+ |
LOC124907872 |
NNNCTCGAGTCATTTCTCTC |
NCG |
2 |
0.0244 |
Tier III |
| 18 |
NC_000013.11 |
13 |
79910744 |
- |
LINC00382 |
NNNCTCGAGTCATTTATCTA |
NGC |
2 |
0.0222 |
Tier III |
| 19 |
NC_000005.10 |
5 |
148510750 |
+ |
LOC107986462 |
NNNCTAGAGTCATTTCTCTA |
NGT |
2 |
0.015 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
16627614 |
- |
Csmd1 |
NNNCTTGAGTCATTTATCTA |
NGG |
2 |
0.9286 |
Tier II |
| 2 |
NC_000072.6 |
6 |
8024588 |
- |
Col28a1 |
NNNCTCGAGTCATTTCTTTA |
NGG |
1 |
0.6429 |
Tier II |
| 3 |
NC_000067.6 |
1 |
36801773 |
- |
Tmem131 |
NNNCTCCAGTCACTTCTCTA |
NGG |
2 |
0.5428 |
Tier II |
| 4 |
NC_000083.6 |
17 |
12760731 |
- |
Igf2r |
NNNCTCCAGTCATTTCTTTA |
NGG |
2 |
0.442 |
Tier II |
| 5 |
NC_000072.6 |
6 |
52593320 |
+ |
Hibadh |
NNNCTCTAGTCATTTCTCTT |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000073.6 |
7 |
83161888 |
+ |
A530021J07Rik |
NNNCTCTAGTCATTTCTGTA |
NGG |
2 |
0.0762 |
Tier II |
| 7 |
NC_000084.6 |
18 |
25423471 |
- |
AW554918 |
NNNCTCCAGTCATTTCTCTA |
NGA |
2 |
0.0477 |
Tier II |
| 8 |
NC_000083.6 |
17 |
12760731 |
- |
Airn |
NNNCTCCAGTCATTTCTTTA |
NGG |
2 |
0.442 |
Tier III |
| 9 |
NC_000078.6 |
12 |
82108325 |
+ |
Gm30799 |
NNNCTCCAGTCATTTCTCTA |
NGA |
2 |
0.0477 |
Tier III |
Other clones with same target sequence:
(none)
