Construct: sgRNA BRDN0001149269

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCATCTAGAACACATTGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHUK (1147)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77034

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 100209615 + CHUK NNNATCTAGAACACATTGAG NGG 0 1.0 Tier I
2 NC_000022.11 22 35777366 + RBFOX2 NNNATATAGAACACATTTAG NGG 2 0.619 Tier II
3 NC_000010.11 10 37759447 - ZNF248 NNNATCTGGAACACATTAAG NGG 2 0.5077 Tier II
4 NC_000002.12 2 152508728 + FMNL2 NNNATCCAGAACATATTGAG NGG 2 0.5042 Tier II
5 NC_000007.14 7 18788954 - HDAC9 NNNATATAGAATACATTGAG NGG 2 0.5 Tier II
6 NC_000011.10 11 46656887 + ATG13 NNNATATAGAATACATTGAG NGG 2 0.5 Tier II
7 NC_000006.12 6 124178918 - NKAIN2 NNNATCTGGAACACATTTAG NGG 2 0.4889 Tier II
8 NC_000003.12 3 96969782 + EPHA6 NNNATCTAGTATACATTGAG NGG 2 0.4751 Tier II
9 NC_000017.11 17 29416598 + TAOK1 NNNAAATAGAACACATTGAG NGG 2 0.4643 Tier II
10 NC_000003.12 3 85847228 + CADM2 NNNATCTAGAACACACTAAG NGG 2 0.4615 Tier II
11 NC_000015.10 15 51225032 - CYP19A1 NNNATCTAAAGCACATTGAG NGG 2 0.4179 Tier II
12 NC_000002.12 2 178449181 + PRKRA NNNGTCTAAAACACATTGAG NGG 2 0.4018 Tier II
13 NC_000010.11 10 76170208 - LRMDA NNNGTCTAAAACACATTGAG NGG 2 0.4018 Tier II
14 NC_000006.12 6 138244610 + ARFGEF3 NNNAACTAGAACACATTTAG NGG 2 0.3333 Tier II
15 NC_000012.12 12 72443607 + TRHDE NNNATGGAGAACACATTGAG NGG 2 0.2941 Tier II
16 NC_000004.12 4 109604899 - MCUB NNNATCTAGAACACATTGAA NAG 2 0.2431 Tier II
17 NC_000006.12 6 30644502 - ATAT1 NNNATCTAGCACACATTCAG NGG 2 0.1587 Tier II
18 NC_000019.10 19 41760569 - CEACAM6 NNNATCTAGAAGACAGTGAG NGG 2 0.1538 Tier II
19 NC_000003.12 3 188227561 + LPP NNNATCTAGAACAAAGTGAG NGG 2 0.1212 Tier II
20 NC_000005.10 5 78127626 + AP3B1 NNNATCTAGAACACTTTGTG NGG 2 0.1077 Tier II
21 NC_000008.11 8 31920467 + NRG1 NNNATCTAAAACACATTGAG NTG 2 0.025 Tier II
22 NC_000015.10 15 51225032 - MIR4713HG NNNATCTAAAGCACATTGAG NGG 2 0.4179 Tier III
23 NC_000010.11 10 120795424 + WDR11-DT NNNGACTAGAACACATTGAG NGG 2 0.3125 Tier III
24 NC_000010.11 10 120795424 + LINC02930 NNNGACTAGAACACATTGAG NGG 2 0.3125 Tier III
25 NC_000001.11 1 193614840 - LOC124904475 NNNCACTAGAACACATTGAG NGG 2 0.1765 Tier III
26 NC_000018.10 18 67623923 - DSEL-AS1 NNNTTCTAGAACACATTGAG NGA 2 0.0442 Tier III
27 NC_000011.10 11 97286396 - LOC105369450 NNNTTCTAGAACACATTGAG NGT 2 0.0103 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 44090953 + Chuk NNNATCTAGAACACACTGAG NGG 1 0.6667 Tier I
2 NC_000068.7 2 80116580 + Pde1a NNNATCTAGGACACAATGAG NGG 2 0.5051 Tier II
3 NC_000076.6 10 31820930 - Nkain2 NNNATCTAGGACACAATGAG NGG 2 0.5051 Tier II
4 NC_000078.6 12 81895740 - Pcnx NNNATTTACAACACATTGAG NGG 2 0.5 Tier II
5 NC_000076.6 10 62497161 + Srgn NNNTTCCAGAACACATTGAG NGG 2 0.4375 Tier II
6 NC_000081.6 15 18928945 + Cdh10 NNNATCTAAAACACATAGAG NGG 2 0.3429 Tier II
7 NC_000076.6 10 59199181 + Septin10 NNNATCTTGAACACATTGAT NGG 2 0.3 Tier II
8 NC_000083.6 17 44582565 + Runx2 NNNAGCTAGAACACATTGAC NGG 2 0.2743 Tier II
9 NC_000082.6 16 55883415 + Nxpe3 NNNATCTAGCACACACTGAG NGG 2 0.2222 Tier II
10 NC_000067.6 1 74546849 + Plcd4 NNNATCTAGAACTCATTAAG NGG 2 0.2077 Tier II
11 NC_000067.6 1 88078026 - Ugt1a10 NNNATCCAGAACACATTGAG NAG 2 0.1782 Tier II
12 NC_000067.6 1 88078026 - Ugt1a9 NNNATCCAGAACACATTGAG NAG 2 0.1782 Tier II
13 NC_000076.6 10 28477691 + Ptprk NNNATCCAGAACACATTGAG NAG 2 0.1782 Tier II
14 NC_000068.7 2 38499345 - Nek6 NNNATCTAGCAGACATTGAG NGG 2 0.1481 Tier II
15 NC_000082.6 16 97066997 - Dscam NNNATCTAGCAGACATTGAG NGG 2 0.1481 Tier II
16 NC_000077.6 11 76578102 - Abr NNNATCTAGAACACCTTGTG NGG 2 0.1469 Tier II
17 NC_000086.7 X 56035516 + 3830403N18Rik NNNATCTAGAACACATAGAG NAG 2 0.1383 Tier II
18 NC_000080.6 14 10812042 + Fhit NNNAGCTAGAACACATGGAG NGG 2 0.0753 Tier II
19 NC_000080.6 14 86907024 - Diaph3 NNNATCTAGAACACATTGAG NCT 2 0.0 Tier II
20 NC_000084.6 18 47936700 - G630055G22Rik NNNATCTAGAACACAATGAG NAG 2 0.2357 Tier III
21 NC_000082.6 16 55883415 + Gm17809 NNNATCTAGCACACACTGAG NGG 2 0.2222 Tier III
22 NC_000084.6 18 11823052 + Gm6277 NNNATCTAGAAGAAATTGAG NGG 2 0.1556 Tier III
23 NC_000068.7 2 38499345 - Gm39799 NNNATCTAGCAGACATTGAG NGG 2 0.1481 Tier III
24 NC_000072.6 6 40811913 - Mgam2-ps NNNATCTAGGACACATTGAG NAG 2 0.144 Tier III
25 NC_000070.6 4 50882869 - Gm42287 NNNATCTAGAACTCATTGAG NGA 2 0.0208 Tier III
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Other clones with same target sequence:

(none)