Construct: sgRNA BRDN0001149281
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCTATCGGCATTCCCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80203
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
35043869 |
- |
SPATA31G1 |
NNNTCTCTGCATTCCCACTG |
NGG |
2 |
0.3125 |
Tier I |
| 2 |
NC_000020.11 |
20 |
48926070 |
+ |
ARFGEF2 |
NNNTATCATCATTCCCACTG |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000010.11 |
10 |
82858795 |
+ |
NRG3 |
NNNTATCGGTAGTCCCACTG |
NGG |
2 |
0.4706 |
Tier II |
| 4 |
NC_000019.10 |
19 |
57720196 |
- |
ZNF671 |
NNNTATGGACATTCCCACTG |
NGG |
2 |
0.3025 |
Tier II |
| 5 |
NC_000015.10 |
15 |
55335889 |
+ |
PIGB |
NNNTATCGGCTTTACCACTG |
NGG |
2 |
0.1077 |
Tier II |
| 6 |
NC_000005.10 |
5 |
54612604 |
+ |
SNX18 |
NNNTATCGGCATACGCACTG |
NGG |
2 |
0.0346 |
Tier II |
| 7 |
NC_000008.11 |
8 |
68529535 |
- |
C8orf34 |
NNNTATCAGCATTCCCACTG |
NGT |
2 |
0.0161 |
Tier II |
| 8 |
NC_000001.11 |
1 |
121431329 |
- |
LINC02798 |
NNNTATCGTCATTCCCACTG |
NGC |
2 |
0.0119 |
Tier II |
| 9 |
NC_000001.11 |
1 |
67749745 |
+ |
GNG12 |
NNNTATCGGCATTCCCAGTG |
NGA |
2 |
0.0093 |
Tier II |
| 10 |
NC_000015.10 |
15 |
87173362 |
+ |
LOC105370955 |
NNNTGTCAGCATTCCCACTG |
NGG |
2 |
0.72 |
Tier III |
| 11 |
NC_000008.11 |
8 |
121680341 |
- |
LINC02855 |
NNNTATCGGCACTTCCACTG |
NGG |
2 |
0.6947 |
Tier III |
| 12 |
NC_000023.11 |
X |
103727151 |
- |
GLRA4 |
NNNTAGCAGCATTCCCACTG |
NGG |
2 |
0.5714 |
Tier III |
| 13 |
NC_000003.12 |
3 |
171534568 |
+ |
MTCO1P58 |
NNNTATCGCCATTCCCACTG |
NCG |
2 |
0.0577 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
73103949 |
- |
Fryl |
NNNTGTCTGCATTCCCACTG |
NGG |
2 |
0.45 |
Tier II |
| 2 |
NC_000075.6 |
9 |
29804741 |
+ |
Ntm |
NNNAATCGGCATTCCCACTG |
NAG |
2 |
0.1235 |
Tier II |
| 3 |
NC_000080.6 |
14 |
25521582 |
+ |
Zmiz1 |
NNNTTTCGGCATTCTCACTG |
NGG |
2 |
0.0242 |
Tier II |
| 4 |
NC_000082.6 |
16 |
18171699 |
- |
Gm34679 |
NNNTGTCAGCATTCCCACTG |
NGG |
2 |
0.72 |
Tier III |
Other clones with same target sequence:
(none)
