Construct: sgRNA BRDN0001149287

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTAATACTATTGAGTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK3 (51347)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76735

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 118243449 - TAOK3 NNNAATACTATTGAGTACAA NGG 0 1.0 Tier I
2 NC_000001.11 1 62525574 + DOCK7 NNNAATACAATTGAGAACAA NGG 2 0.8442 Tier II
3 NC_000005.10 5 132632203 - RAD50 NNNAATATTATTAAGTACAA NGG 2 0.8077 Tier II
4 NC_000003.12 3 105540826 - ALCAM NNNAATACTATTGAGTACTG NGG 2 0.4118 Tier II
5 NC_000005.10 5 38528123 - LIFR NNNAAGAATATTGAGTACAA NGG 2 0.3714 Tier II
6 NC_000015.10 15 53524243 + WDR72 NNNGATACTGTTGAGTACAA NGG 2 0.3472 Tier II
7 NC_000001.11 1 173543935 + SLC9C2 NNNAATACTATTCAGTACAT NGG 2 0.2526 Tier II
8 NC_000005.10 5 75875510 + SV2C NNNAATACTATTCAGTACAT NGG 2 0.2526 Tier II
9 NC_000003.12 3 196402012 - UBXN7 NNNAATACAATTGAGTACAA NAG 2 0.2407 Tier II
10 NC_000008.11 8 118369850 + SAMD12 NNNAATACTATTGAGGACAT NGG 2 0.2077 Tier II
11 NC_000005.10 5 120704542 - PRR16 NNNAATACTCTTGAGTACAT NGG 2 0.2 Tier II
12 NC_000010.11 10 54315613 - PCDH15 NNNAATACTATTCAGGACAA NGG 2 0.1457 Tier II
13 NC_000012.12 12 100934182 - ANO4 NNNAATACTATTCAGGACAA NGG 2 0.1457 Tier II
14 NC_000006.12 6 114292165 - HS3ST5 NNNAATACTATTGTCTACAA NGG 2 0.1455 Tier II
15 NC_000023.11 X 134385497 - PHF6 NNNAACACTATTGAGTACAA NGA 2 0.0631 Tier II
16 NC_000006.12 6 151335886 - AKAP12 NNNAATAGTATTGAGTACAA NGT 2 0.0104 Tier II
17 NC_000018.10 18 74259963 - CYB5A NNNAATACTATTTAGTACAA NGC 2 0.0067 Tier II
18 NC_000008.11 8 14400749 - SGCZ NNNAATACTATTGATTACAA NGT 2 0.0023 Tier II
19 NC_000004.12 4 89942344 - MMRN1 NNNAATACTATTGAGTACAA NTT 2 0.0 Tier II
20 NC_000015.10 15 45785912 + LOC105370802 NNNAATATTATTGAATACAA NGG 2 0.8235 Tier III
21 NC_000005.10 5 132632203 - TH2LCRR NNNAATATTATTAAGTACAA NGG 2 0.8077 Tier III
22 NC_000002.12 2 4138202 + LOC107985841 NNNAATACTATAAAGTACAA NGG 2 0.7385 Tier III
23 NC_000006.12 6 91725072 - LOC105377894 NNNAATACTATTGAGAACAT NGG 2 0.5455 Tier III
24 NC_000002.12 2 167910469 + B3GALT1-AS1 NNNAATACTAAGGAGTACAA NGG 2 0.375 Tier III
25 NC_000015.10 15 53524243 + LOC105370826 NNNGATACTGTTGAGTACAA NGG 2 0.3472 Tier III
26 NC_000012.12 12 16832835 + LOC105369677 NNNAATAATATTGTGTACAA NGG 2 0.3467 Tier III
27 NC_000002.12 2 40556630 - LOC101929667 NNNAATACTATAGAGGACAA NGG 2 0.2769 Tier III
28 NC_000008.11 8 118369850 + LOC105375724 NNNAATACTATTGAGGACAT NGG 2 0.2077 Tier III
29 NC_000016.10 16 72585860 + LINC01572 NNNAATACTATTCAGGACAA NGG 2 0.1457 Tier III
30 NC_000006.12 6 114292165 - HDAC2-AS2 NNNAATACTATTGTCTACAA NGG 2 0.1455 Tier III
31 NC_000006.12 6 8288482 + LOC105374911 NNNAATACTAATGAGTGCAA NGG 2 0.1324 Tier III
32 NC_000002.12 2 101309628 + LOC105373512 NNNATTACTATTGAGTACAA NAG 2 0.0943 Tier III
33 NC_000008.11 8 95620065 + CFAP418-AS1 NNNAATACTATTGACTACAA NAG 2 0.0707 Tier III
34 NC_000008.11 8 5851677 + LOC107986908 NNNAAAACTATTGAGTACAA NGT 2 0.014 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 46717647 + Nova1 NNNAATACTATCAAGTACAA NGG 2 0.8745 Tier II
2 NC_000069.6 3 37444069 - Spata5 NNNTACACTATTGAGTACAA NGG 2 0.5785 Tier II
3 NC_000072.6 6 106331526 + Cntn4 NNNAATACTGTTAAGTACAA NGG 2 0.5128 Tier II
4 NC_000069.6 3 69114839 - Kpna4 NNNTATAGTATTGAGTACAA NGG 2 0.4091 Tier II
5 NC_000069.6 3 158472418 + Lrrc7 NNNAATACTATTCAATACAA NGG 2 0.3963 Tier II
6 NC_000083.6 17 74678112 - Birc6 NNNAACACTCTTGAGTACAA NGG 2 0.303 Tier II
7 NC_000085.6 19 11987255 - Osbp NNNAATACTATGGAGTAAAA NGG 2 0.2692 Tier II
8 NC_000079.6 13 90939506 + Atg10 NNNTTTACTATTGAGTACAA NGG 2 0.2314 Tier II
9 NC_000068.7 2 73877473 - Atf2 NNNATTACTATTGAGTACTA NGG 2 0.1958 Tier II
10 NC_000081.6 15 35081753 + Stk3 NNNAATACTATTGAATGCAA NGG 2 0.1661 Tier II
11 NC_000083.6 17 72544207 + Alk NNNAATGCTATTGAGTTCAA NGG 2 0.0941 Tier II
12 NC_000069.6 3 129817026 + Rrh NNNATTACTATTGAGTACAC NGG 2 0.0826 Tier II
13 NC_000078.6 12 57020747 - Slc25a21 NNNAATACTATTGAGTACTA NTG 2 0.021 Tier II
14 NC_000074.6 8 116798000 - 4930563M20Rik NNNAAAACTATAGAGTACAA NGG 2 0.6933 Tier III
15 NC_000081.6 15 42923291 + Gm46505 NNNAATACTTTTGAGTATAA NGG 2 0.5672 Tier III
16 NC_000080.6 14 122405758 - Gm5089 NNNAAAACTATTGAGTGCAA NGG 2 0.1529 Tier III
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Other clones with same target sequence:

(none)