Construct: sgRNA BRDN0001149294
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGACGTGCTGGCGTCACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80192
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
100145518 |
+ |
FBXO43 |
NNNGATGGGCTGGCGTCACC |
NGG |
2 |
0.681 |
Tier II |
| 2 |
NC_000019.10 |
19 |
14006190 |
+ |
RFX1 |
NNNGACGTGCTGGGGTCCCC |
NGG |
2 |
0.0 |
Tier II |
| 3 |
NC_000017.11 |
17 |
20615978 |
- |
LINC02088 |
NNNGAGGTGCTGGCGGCACC |
NGG |
2 |
0.1731 |
Tier III |
| 4 |
NC_000013.11 |
13 |
108361919 |
+ |
LOC105370355 |
NNNGACGTGCTGGAGTGACC |
NGG |
2 |
0.0206 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
118223334 |
+ |
Ptprf |
NNNGCTGTGCTGGCGTCACC |
NGG |
2 |
0.4643 |
Tier I |
| 2 |
NC_000076.6 |
10 |
58224446 |
- |
LOC100504180 |
NNNGACTTGCTGGCGTCACA |
NGG |
2 |
0.2857 |
Tier I |
| 3 |
NC_000076.6 |
10 |
58232125 |
- |
Duxf3 |
NNNGACTTGCTGGCGTCACA |
NGG |
2 |
0.2857 |
Tier I |
| 4 |
NC_000068.7 |
2 |
25567351 |
+ |
Mamdc4 |
NNNGACGTGCGGGCCTCACC |
NGG |
2 |
0.1091 |
Tier I |
| 5 |
NC_000075.6 |
9 |
114026756 |
- |
Fbxl2 |
NNNGTCGGGCTGGCGTCACC |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000071.6 |
5 |
100871566 |
+ |
Gpat3 |
NNNGACATGCTGGCTTCACC |
NGG |
2 |
0.1429 |
Tier II |
| 7 |
NC_000076.6 |
10 |
58240255 |
- |
Gm9919 |
NNNGACTTGCTGGCGTCACA |
NGG |
2 |
0.2857 |
Tier III |
Other clones with same target sequence:
(none)
