Construct: sgRNA BRDN0001149337
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAATCTCAATGAGCTACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K5 (11183)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75712
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
50440382 |
- |
MAP4K5 |
NNNTCTCAATGAGCTACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
42955977 |
+ |
AARSD1 |
NNNTCTTAATGACCTACATG |
NGG |
2 |
0.3421 |
Tier I |
| 3 |
NC_000017.11 |
17 |
42955977 |
+ |
PTGES3L-AARSD1 |
NNNTCTTAATGACCTACATG |
NGG |
2 |
0.3421 |
Tier I |
| 4 |
NC_000001.11 |
1 |
224361889 |
- |
CNIH4 |
NNNTCTCAAGAAGCTACATG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000012.12 |
12 |
39310979 |
- |
KIF21A |
NNNTCTCAATGAGATACATG |
NTG |
2 |
0.0136 |
Tier II |
| 6 |
NC_000002.12 |
2 |
118136286 |
+ |
LOC124907879 |
NNNTCCCAATGGGCTACATG |
NGG |
2 |
0.6566 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
69823442 |
- |
Map4k5 |
NNNTCTCAATGAGCTACATG |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000068.7 |
2 |
152824870 |
+ |
Bcl2l1 |
NNNTCAGAATGAGCTACATG |
NGG |
2 |
0.4078 |
Tier II |
| 3 |
NC_000072.6 |
6 |
105974552 |
- |
Cntn4 |
NNNACTTAATGAGCTACATG |
NGG |
2 |
0.3869 |
Tier II |
| 4 |
NC_000070.6 |
4 |
98544743 |
+ |
Patj |
NNNTCTCCATGGGCTACATG |
NGG |
2 |
0.3095 |
Tier II |
| 5 |
NC_000081.6 |
15 |
10318634 |
+ |
Prlr |
NNNTCTTAATGAGCCACATG |
NGG |
2 |
0.2216 |
Tier II |
| 6 |
NC_000083.6 |
17 |
70790505 |
- |
Dlgap1 |
NNNTCTCCATGAGCTACATG |
NAG |
2 |
0.1111 |
Tier II |
| 7 |
NC_000074.6 |
8 |
102634950 |
+ |
Cdh11 |
NNNTCTCAAAGAGCTACATG |
NTG |
2 |
0.0334 |
Tier II |
| 8 |
NC_000075.6 |
9 |
107013931 |
- |
Dock3 |
NNNACTCAATGAGCTAGATG |
NGG |
2 |
0.028 |
Tier II |
| 9 |
NC_000070.6 |
4 |
47238950 |
- |
Col15a1 |
NNNCCTCAATGAGCTACATG |
NGT |
2 |
0.0104 |
Tier II |
| 10 |
NC_000073.6 |
7 |
57496466 |
- |
Gabra5 |
NNNTCTCAATGAGATTCATG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000074.6 |
8 |
54904891 |
+ |
Gpm6a |
NNNTCTCATTGAGCTTCATG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000073.6 |
7 |
94440777 |
- |
Gm32647 |
NNNTTGCAATGAGCTACATG |
NGG |
2 |
0.3636 |
Tier III |
| 13 |
NC_000078.6 |
12 |
69823442 |
- |
4930512B01Rik |
NNNTCTCAATGAGCTACATG |
NAG |
1 |
0.2593 |
Tier III |
| 14 |
NC_000083.6 |
17 |
70790505 |
- |
Gm41609 |
NNNTCTCCATGAGCTACATG |
NAG |
2 |
0.1111 |
Tier III |
| 15 |
NC_000070.6 |
4 |
47238950 |
- |
Gm32317 |
NNNCCTCAATGAGCTACATG |
NGT |
2 |
0.0104 |
Tier III |
| 16 |
NC_000075.6 |
9 |
17221042 |
- |
Gm39296 |
NNNTCTCATTGAGCTTCATG |
NGG |
2 |
0.0 |
Tier III |
| 17 |
NC_000082.6 |
16 |
86248793 |
- |
Gm32357 |
NNNTCTCATTGAGCTTCATG |
NGG |
2 |
0.0 |
Tier III |
| 18 |
NC_000073.6 |
7 |
82969750 |
- |
4933430H16Rik |
NNNTCTTAATGAGCTTCATG |
NGG |
2 |
0.0 |
Tier III |
| 19 |
NC_000078.6 |
12 |
115660483 |
+ |
Igh |
NNNTCTTAATGAGCTTCATG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
