Construct: sgRNA BRDN0001149467

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGTCCAGATGGCTTACAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERBB4 (2066)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76196

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 211665402 - ERBB4 NNNTCCAGATGGCTTACAGG NGG 0 1.0 Tier I
2 NC_000016.10 16 55856469 - CES5A NNNACCAGATGGCTTACAGA NGG 2 0.4464 Tier II
3 NC_000007.14 7 128034745 - SND1 NNNCCCAGCTGGCTTACAGG NGG 2 0.3697 Tier II
4 NC_000006.12 6 170546366 + PSMB1 NNNTCCAGATGAATTACAGG NGG 2 0.359 Tier II
5 NC_000020.11 20 16373936 + KIF16B NNNTCCAGAGGGCTAACAGG NGG 2 0.2895 Tier II
6 NC_000012.12 12 120811373 + SPPL3 NNNTCTAGATGGCCTACAGG NGG 2 0.2653 Tier II
7 NC_000016.10 16 81010820 - CENPN NNNTCCAGATGGATTACATG NGG 2 0.2564 Tier II
8 NC_000011.10 11 68266349 - C11orf24 NNNTCCACATGGATTACAGG NGG 2 0.2367 Tier II
9 NC_000001.11 1 224546304 + CNIH3 NNNTCCAGAAGGCTTACAGG NAG 2 0.2222 Tier II
10 NC_000001.11 1 220234320 + RAB3GAP2 NNNTCCTGATGGCTTACAGC NGG 2 0.1875 Tier II
11 NC_000001.11 1 14326325 - KAZN NNNTCCAGATGGGTTACATG NGG 2 0.0909 Tier II
12 NC_000003.12 3 36390996 + STAC NNNTCCAGATGGGTTATAGG NGG 2 0.0636 Tier II
13 NC_000020.11 20 19935566 + RIN2 NNNTCCAGAAGGCTTACAGG NGA 2 0.0595 Tier II
14 NC_000008.11 8 103657275 - RIMS2 NNNTCCAGATGGCTTACAAG NGA 2 0.0496 Tier II
15 NC_000002.12 2 235008961 + SH3BP4 NNNTCCAGCTGGCTTACAGG NGC 2 0.0127 Tier II
16 NC_000008.11 8 132328136 + KCNQ3 NNNTCCAGATGGCTTGGAGG NGG 2 0.0113 Tier II
17 NC_000004.12 4 5741190 + EVC NNNTCCAGATGTCTTACAGG NGT 2 0.0062 Tier II
18 NC_000003.12 3 192535508 - FGF12 NNNTCCAGATGGCTCACAGG NGC 2 0.0061 Tier II
19 NC_000007.14 7 151709989 + PRKAG2 NNNTCCAGAGGGCTTTCAGG NGG 2 0.0 Tier II
20 NC_000003.12 3 58415795 + PXK NNNTCCAGATGGCTTACAGG NAA 2 0.0 Tier II
21 NC_000005.10 5 55391890 - MTREX NNNTCCAGATGGCTTACAGG NTA 2 0.0 Tier II
22 NC_000001.11 1 245237392 - KIF26B NNNTCCAGATGGCTTCCAGG NGC 2 0.0 Tier II
23 NC_000004.12 4 15793692 - CD38 NNNTCCAGATGGCTTCCAGG NTG 2 0.0 Tier II
24 NC_000001.11 1 62292505 - KANK4 NNNTCCAGATGGCTTCCTGG NGG 2 0.0 Tier II
25 NC_000011.10 11 19800350 + NAV2 NNNTCCAGTTGGCTTCCAGG NGG 2 0.0 Tier II
26 NC_000013.11 13 25112871 - LOC124900613 NNNTCCAAATGGCATACAGG NGG 2 0.619 Tier III
27 NC_000001.11 1 19301621 - LOC124903867 NNNTCCAGAGGGCATACAGG NGG 2 0.3095 Tier III
28 NC_000003.12 3 38158795 + LOC101928234 NNNCCCAGATGGCTCACAGG NGG 2 0.1765 Tier III
29 NC_000003.12 3 36390996 + LOC124906227 NNNTCCAGATGGGTTATAGG NGG 2 0.0636 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 33389227 - Purg NNNTCCAGATGGCTCACAAG NGG 2 0.1948 Tier I
2 NC_000067.6 1 68298366 - Erbb4 NNNTCCAGATGGCCTACAGG NAG 2 0.0741 Tier I
3 NC_000076.6 10 77706876 - Gm10272 NNNTCCAGATGACTGACAGG NGG 2 0.0467 Tier I
4 NC_000076.6 10 77711737 - Gm10024 NNNTCCAGATGACTGACAGG NGG 2 0.0467 Tier I
5 NC_000074.6 8 105677264 + Ctcf NNNTCCAGATGGCGTAGAGG NGG 2 0.0 Tier I
6 NC_000078.6 12 53321138 + Npas3 NNNTCCAGATGATTTACAGG NGG 2 0.6533 Tier II
7 NC_000071.6 5 44473959 + Ldb2 NNNTGCAGATAGCTTACAGG NGG 2 0.6 Tier II
8 NC_000081.6 15 59007771 - Mtss1 NNNCCCAGAAGGCTTACAGG NGG 2 0.5546 Tier II
9 NC_000076.6 10 5504979 - Syne1 NNNTCCAGATGGCAAACAGG NGG 2 0.3584 Tier II
10 NC_000073.6 7 57247567 - Gabrg3 NNNTCCAGATGGTTTACTGG NGG 2 0.35 Tier II
11 NC_000079.6 13 117712433 - Hcn1 NNNTCCAGATGGCTTAAAAG NGG 2 0.3333 Tier II
12 NC_000082.6 16 9684230 - Grin2a NNNTACAGCTGGCTTACAGG NGG 2 0.3265 Tier II
13 NC_000073.6 7 127010871 - Mvp NNNTCCAGATGGCTTATAGC NGG 2 0.2 Tier II
14 NC_000085.6 19 45422423 - Btrc NNNTCCAGATGGATTAAAGG NGG 2 0.1795 Tier II
15 NC_000080.6 14 28507715 - Wnt5a NNNTCCAGATGGCCAACAGG NGG 2 0.1654 Tier II
16 NC_000070.6 4 155267338 - Prkcz NNNTCCAGATGGCTTACAGC NAG 2 0.1111 Tier II
17 NC_000076.6 10 77706876 - Tspear NNNTCCAGATGACTGACAGG NGG 2 0.0467 Tier II
18 NC_000076.6 10 77711737 - Tspear NNNTCCAGATGACTGACAGG NGG 2 0.0467 Tier II
19 NC_000077.6 11 113376733 + Slc39a11 NNNTCCAGATGGCTTAAAGG NGA 2 0.0324 Tier II
20 NC_000070.6 4 155991350 + B3galt6 NNNTCCAGATGGCTGATAGG NGG 2 0.0233 Tier II
21 NC_000067.6 1 52158683 - Stat1 NNNTCCAGATGTCTTAGAGG NGG 2 0.0226 Tier II
22 NC_000080.6 14 7960675 - Gm45521 NNNTCCAGATGGATGACAGG NGG 2 0.0192 Tier II
23 NC_000074.6 8 69869914 - Pbx4 NNNTCCAGATGGCTTAAAGG NGC 2 0.0104 Tier II
24 NC_000077.6 11 119498868 - Endov NNNACCAGATGGCTTTCAGG NGG 2 0.0 Tier II
25 NC_000072.6 6 38993008 + Tbxas1 NNNTCCAGATGACTTTCAGG NGG 2 0.0 Tier II
26 NC_000075.6 9 36984859 - Pknox2 NNNTCCAGATGCCTTCCAGG NGG 2 0.0 Tier II
27 NC_000073.6 7 16013894 - Bicra NNNTCCAGATGGCTTCCCGG NGG 2 0.0 Tier II
28 NC_000067.6 1 132719009 - Nfasc NNNTCCAGATGGCTTTCAGG NGT 2 0.0 Tier II
29 NC_000073.6 7 17752449 + Ceacam5 NNNTCTAGATGGCTTCCAGG NGG 2 0.0 Tier II
30 NC_000079.6 13 117712433 - 4933413L06Rik NNNTCCAGATGGCTTAAAAG NGG 2 0.3333 Tier III
31 NC_000069.6 3 51071202 - Gm29230 NNNTCCAGGGGGCTTACAGG NGG 2 0.3333 Tier III
32 NC_000072.6 6 38993008 + Gm38688 NNNTCCAGATGACTTTCAGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)