Construct: sgRNA BRDN0001149476

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AATATTCTAATAACTAAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL4 (344387)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76108

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 39204574 - CDKL4 NNNATTCTAATAACTAAGCA NGG 0 1.0 Tier I
2 NC_000004.12 4 80584056 - CFAP299 NNNATTCTATTAACTAAACA NGG 2 0.6109 Tier II
3 NC_000012.12 12 72251719 - TRHDE NNNTTTCTAAAAACTAAGCA NGG 2 0.4773 Tier II
4 NC_000017.11 17 33261216 - ASIC2 NNNATTCTGATAACTAAGCT NGG 2 0.4 Tier II
5 NC_000012.12 12 130357259 + PIWIL1 NNNATTTTAATAACTAAGAA NGG 2 0.3482 Tier II
6 NC_000011.10 11 96387098 + CCDC82 NNNATTGTAATAACAAAGCA NGG 2 0.2724 Tier II
7 NC_000004.12 4 7842485 + AFAP1 NNNATTCTAATAACCAACCA NGG 2 0.1299 Tier II
8 NC_000007.14 7 55074831 + EGFR NNNATTCTAATTACCAAGCA NGG 2 0.0909 Tier II
9 NC_000006.12 6 144791636 - UTRN NNNATTCTAATAAATAAGCA NAG 2 0.0907 Tier II
10 NC_000023.11 X 70354099 + KIF4A NNNATTCTAATAACTATGCT NGG 2 0.08 Tier II
11 NC_000008.11 8 105431349 - ZFPM2 NNNATTCTAATAACCAAGCA NAG 2 0.0707 Tier II
12 NC_000002.12 2 159348641 + BAZ2B NNNATTCTCATAACTAAGCA NGA 2 0.0397 Tier II
13 NC_000003.12 3 76982839 - ROBO2 NNNATTCTACTAACGAAGCA NGG 2 0.0167 Tier II
14 NC_000015.10 15 49494944 + FAM227B NNNATTCTAATACCTAAGCA NGA 2 0.0146 Tier II
15 NC_000003.12 3 119802059 + NR1I2 NNNATTCTATTAACTAAGCA NGT 2 0.0142 Tier II
16 NC_000011.10 11 78965484 - TENM4 NNNATTCTAACAACTCAGCA NGG 2 0.0 Tier II
17 NC_000007.14 7 16328324 + CRPPA NNNATTCTAATAACTAAGCA NAC 2 0.0 Tier II
18 NC_000006.12 6 80237391 + BCKDHB NNNATTCTAATAACTTAGAA NGG 2 0.0 Tier II
19 NC_000002.12 2 7943009 - LINC00298 NNNATACTAATAGCTAAGCA NGG 2 0.5652 Tier III
20 NC_000012.12 12 46573645 + SLC38A4-AS1 NNNATTCTAAAAGCTAAGCA NGG 2 0.4891 Tier III
21 NC_000008.11 8 90481874 - LOC124901975 NNNATTCTAATAGTTAAGCA NGG 2 0.4783 Tier III
22 NC_000002.12 2 184363631 + LOC102724340 NNNATTCTATTAAATAAGCA NGG 2 0.3088 Tier III
23 NC_000002.12 2 184363631 + LOC105373776 NNNATTCTATTAAATAAGCA NGG 2 0.3088 Tier III
24 NC_000007.14 7 130735600 - LOC105375508 NNNATTTTAATAAATAAGCA NGG 2 0.2844 Tier III
25 NC_000015.10 15 96699011 + LOC105371002 NNNCTTCTAATATCTAAGCA NGG 2 0.1059 Tier III
26 NC_000008.11 8 64142595 - LINC01414 NNNATTCTAATTCCTAAGCA NGG 2 0.0702 Tier III
27 NC_000010.11 10 61859334 - LINC02625 NNNATTCTCATAACTCAGCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 80547739 - Cdkl4 NNNATCCTAATAACCAAGCA NGG 2 0.2479 Tier I
2 NC_000084.6 18 36157235 - Nrg2 NNNATACTATTAACTAAGCA NGG 2 0.7647 Tier II
3 NC_000083.6 17 3683818 - Nox3 NNNATACTAATGACTAAGCA NGG 2 0.6259 Tier II
4 NC_000071.6 5 74590123 - Fip1l1 NNNAGTCTAATGACTAAGCA NGG 2 0.4622 Tier II
5 NC_000079.6 13 8428779 - Adarb2 NNNATTCTAATAACTAAGAA NGG 1 0.4286 Tier II
6 NC_000072.6 6 46113343 - Cntnap2 NNNCTTCTAATAACTAAGCA NGG 1 0.3529 Tier II
7 NC_000071.6 5 89874928 + Adamts3 NNNATTCTAATAACTAAGCA NAG 1 0.2593 Tier II
8 NC_000067.6 1 21773530 + Kcnq5 NNNATTCTAAGAACTAAGAA NGG 2 0.1714 Tier II
9 NC_000076.6 10 13763282 + Aig1 NNNATTCTAATAACTAAGTA NAG 2 0.1197 Tier II
10 NC_000086.7 X 104604546 - Zdhhc15 NNNATTCTACTTACTAAGCA NGG 2 0.1111 Tier II
11 NC_000083.6 17 80702100 + Map4k3 NNNATTCTAATAACAAAGCA NTG 2 0.0226 Tier II
12 NC_000067.6 1 171919660 + Slamf6 NNNATTCTAATAAAGAAGCA NGG 2 0.0175 Tier II
13 NC_000076.6 10 111004019 - Zdhhc17 NNNATTGTAATAACTAAGCA NGC 2 0.0105 Tier II
14 NC_000068.7 2 67884299 + B3galt1 NNNGTTCTAATAACTAAGCA NGT 2 0.0101 Tier II
15 NC_000069.6 3 68943028 - Ift80 NNNATTCTAATAAATAAGCA NGC 2 0.0078 Tier II
16 NC_000072.6 6 43365094 - Tpk1 NNNATTCTAATTACTAAGCA NGT 2 0.0054 Tier II
17 NC_000083.6 17 10254134 + Qk NNNATTCTAATTACTAAGCA NGT 2 0.0054 Tier II
18 NC_000071.6 5 87673069 - Csn1s1 NNNATTCTAATAACTCAGCA NGA 2 0.0 Tier II
19 NC_000073.6 7 33633980 - Scgb2b23-ps NNNTTTCTCATAACTAAGCA NGG 2 0.3636 Tier III
20 NC_000076.6 10 92271957 + Gm20757 NNNATTCTGATAACTAGGCA NGG 2 0.1176 Tier III
21 NC_000076.6 10 14734643 + 1700016L04Rik NNNATTCTAATAACATAGCA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)