Construct: sgRNA BRDN0001149491
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTCTGGGCAAGATGACAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MAP2K7 (5609)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000019.10 | 19 | 7910796 | + | MAP2K7 | NNNTCTGGGCAAGATGACAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000020.11 | 20 | 20614613 | + | RALGAPA2 | NNNTCTGGGAAAGATGACAT | NGG | 2 | 0.6067 | Tier II |
3 | NC_000016.10 | 16 | 83459201 | - | CDH13 | NNNTCTGGACAAAATGACAG | NGG | 2 | 0.5934 | Tier II |
4 | NC_000005.10 | 5 | 167249080 | + | TENM2 | NNNTCTTGGCAAGATGACAG | NGG | 1 | 0.5714 | Tier II |
5 | NC_000023.11 | X | 49189948 | + | SYP | NNNTCTGGGAGAGATGACAG | NGG | 2 | 0.5633 | Tier II |
6 | NC_000016.10 | 16 | 12174384 | + | SNX29 | NNNTCAGGGCAAGAAGACAG | NGG | 2 | 0.5018 | Tier II |
7 | NC_000019.10 | 19 | 7910886 | + | MAP2K7 | NNNACTGGGCAAGATGACAG | NGG | 1 | 0.4762 | Tier II |
8 | NC_000011.10 | 11 | 66605075 | - | CCS | NNNCCTGGGCAGGATGACAG | NGG | 2 | 0.4673 | Tier II |
9 | NC_000005.10 | 5 | 172899072 | + | ERGIC1 | NNNTCTGAGCAAGGTGACAG | NGG | 2 | 0.4667 | Tier II |
10 | NC_000018.10 | 18 | 21422254 | + | GREB1L | NNNTCTGAGCAAGGTGACAG | NGG | 2 | 0.4667 | Tier II |
11 | NC_000019.10 | 19 | 23864166 | + | RPSA2 | NNNTCTGAGCAAGATGACAC | NGG | 2 | 0.4286 | Tier II |
12 | NC_000007.14 | 7 | 45174294 | + | RAMP3 | NNNTCTGGGACAGATGACAG | NGG | 2 | 0.3467 | Tier II |
13 | NC_000019.10 | 19 | 7910841 | + | MAP2K7 | NNNACTGGGCAGGATGACAG | NGG | 2 | 0.3439 | Tier II |
14 | NC_000015.10 | 15 | 74311682 | + | CCDC33 | NNNTCTGGGTTAGATGACAG | NGG | 2 | 0.2896 | Tier II |
15 | NC_000022.11 | 22 | 43592679 | + | EFCAB6 | NNNTCTGGGCAACAAGACAG | NGG | 2 | 0.2438 | Tier II |
16 | NC_000015.10 | 15 | 41502564 | - | ITPKA | NNNACTGGGCCAGATGACAG | NGG | 2 | 0.1905 | Tier II |
17 | NC_000023.11 | X | 15366816 | - | VEGFD | NNNTCTGGGCTAGAAGACAG | NGG | 2 | 0.1781 | Tier II |
18 | NC_000010.11 | 10 | 80521553 | - | TSPAN14 | NNNCCTGGGCACGATGACAG | NGG | 2 | 0.1703 | Tier II |
19 | NC_000012.12 | 12 | 51192151 | - | POU6F1 | NNNTTTGGGCACGATGACAG | NGG | 2 | 0.1675 | Tier II |
20 | NC_000008.11 | 8 | 23530066 | - | SLC25A37 | NNNTCTGGGCAGGCTGACAG | NGG | 2 | 0.1548 | Tier II |
21 | NC_000023.11 | X | 57454093 | - | FAAH2 | NNNTCTGGCCAAGATGACAG | NAG | 2 | 0.1396 | Tier II |
22 | NC_000004.12 | 4 | 2423696 | - | CFAP99 | NNNCCTGGGCAAGATGAGAG | NGG | 2 | 0.0863 | Tier II |
23 | NC_000016.10 | 16 | 48334607 | + | LONP2 | NNNTCTGGGCAAGGTGGCAG | NGG | 2 | 0.0824 | Tier II |
24 | NC_000003.12 | 3 | 57466518 | + | DNAH12 | NNNTCTGGGCATGCTGACAG | NGG | 2 | 0.0714 | Tier II |
25 | NC_000001.11 | 1 | 3110974 | + | PRDM16 | NNNTCTGGGCAAGACGGCAG | NGG | 2 | 0.0481 | Tier II |
26 | NC_000019.10 | 19 | 35708103 | + | ZBTB32 | NNNCCTGGGCAAGATGACAG | NGA | 2 | 0.0449 | Tier II |
27 | NC_000015.10 | 15 | 72291053 | - | CELF6 | NNNTCAGGGCAAGAGGACAG | NGG | 2 | 0.0433 | Tier II |
28 | NC_000007.14 | 7 | 44240827 | - | CAMK2B | NNNTCTGGGCAGGAGGACAG | NGG | 2 | 0.0361 | Tier II |
29 | NC_000011.10 | 11 | 96125531 | + | MAML2 | NNNTCTGGGCAAGTGGACAG | NGG | 2 | 0.0267 | Tier II |
30 | NC_000005.10 | 5 | 119633309 | + | FAM170A | NNNTCTGGGCAAGATGACAG | NGC | 1 | 0.0222 | Tier II |
31 | NC_000009.12 | 9 | 117246652 | + | ASTN2 | NNNTCTGGGGAAGAGGACAG | NGG | 2 | 0.0194 | Tier II |
32 | NC_000002.12 | 2 | 172786851 | - | RAPGEF4 | NNNTCTGGGCTAGAGGACAG | NGG | 2 | 0.0154 | Tier II |
33 | NC_000003.12 | 3 | 165157838 | - | SI | NNNTCTGGGCTAGAGGACAG | NGG | 2 | 0.0154 | Tier II |
34 | NC_000008.11 | 8 | 22982220 | + | RHOBTB2 | NNNCCTGGGCAAGATGACAG | NGT | 2 | 0.0104 | Tier II |
35 | NC_000003.12 | 3 | 10273645 | + | TATDN2 | NNNTCTGGGCAAGATGATAG | NGT | 2 | 0.0104 | Tier II |
36 | NC_000016.10 | 16 | 70494007 | - | COG4 | NNNTCTGGGCAAGATGAGAG | NGA | 2 | 0.0093 | Tier II |
37 | NC_000011.10 | 11 | 19647796 | + | NAV2 | NNNTCTGGGCAAGAGGGCAG | NGG | 2 | 0.0088 | Tier II |
38 | NC_000001.11 | 1 | 169820149 | + | FIRRM | NNNTCTGGGCAAGATGAAAG | NGT | 2 | 0.0087 | Tier II |
39 | NC_000001.11 | 1 | 151806263 | + | RORC | NNNTCTGGGCAAGATCACAG | NTG | 2 | 0.0 | Tier II |
40 | NC_000022.11 | 22 | 38868532 | + | CBX6 | NNNTCTGGGCAAGATCCCAG | NGG | 2 | 0.0 | Tier II |
41 | NC_000016.10 | 16 | 22210939 | + | EEF2K | NNNTCTGGGCAGGATCACAG | NGG | 2 | 0.0 | Tier II |
42 | NC_000003.12 | 3 | 954567 | + | LOC107986059 | NNNTCTGGGCAAGAAAACAG | NGG | 2 | 0.5789 | Tier III |
43 | NC_000018.10 | 18 | 21422254 | + | LOC101927521 | NNNTCTGAGCAAGGTGACAG | NGG | 2 | 0.4667 | Tier III |
44 | NC_000015.10 | 15 | 74311682 | + | LOC124903525 | NNNTCTGGGTTAGATGACAG | NGG | 2 | 0.2896 | Tier III |
45 | NC_000013.11 | 13 | 22220593 | + | LINC00540 | NNNACTGGGCCAGATGACAG | NGG | 2 | 0.1905 | Tier III |
46 | NC_000023.11 | X | 15366816 | - | PIR-FIGF | NNNTCTGGGCTAGAAGACAG | NGG | 2 | 0.1781 | Tier III |
47 | NC_000002.12 | 2 | 108330402 | - | SULT1C5P | NNNTCTTGGCAAGCTGACAG | NGG | 2 | 0.1224 | Tier III |
48 | NC_000016.10 | 16 | 55654298 | - | LOC124903693 | NNNTCTGGGGAAGATGACAG | NAG | 2 | 0.1008 | Tier III |
49 | NC_000016.10 | 16 | 48334607 | + | UBA52P8 | NNNTCTGGGCAAGGTGGCAG | NGG | 2 | 0.0824 | Tier III |
50 | NC_000013.11 | 13 | 53539146 | + | LOC105370210 | NNNTCTGGGCTAGATGCCAG | NGG | 2 | 0.0543 | Tier III |
51 | NC_000007.14 | 7 | 25689716 | + | UBA52P1 | NNNTCTGGGCAAGCTGGCAG | NGG | 2 | 0.0378 | Tier III |
52 | NC_000007.14 | 7 | 25689716 | + | LINC03007 | NNNTCTGGGCAAGCTGGCAG | NGG | 2 | 0.0378 | Tier III |
53 | NC_000002.12 | 2 | 85934698 | - | LOC124906031 | NNNTCTGTGCAAGATGACAG | NTG | 2 | 0.0244 | Tier III |
54 | NC_000008.11 | 8 | 47062951 | - | LOC105375815 | NNNTCTGGGCAAGATGAAAG | NGC | 2 | 0.012 | Tier III |
55 | NC_000008.11 | 8 | 22982220 | + | LOC107984124 | NNNCCTGGGCAAGATGACAG | NGT | 2 | 0.0104 | Tier III |
56 | NC_000006.12 | 6 | 123668946 | + | LOC105377981 | NNNTCTGGGCAAGTTCACAG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000074.6 | 8 | 4244329 | + | Map2k7 | NNNCCTGGGCAAGATGACTG | NGG | 2 | 0.3484 | Tier I |
2 | NC_000068.7 | 2 | 167471212 | - | Slc9a8 | NNNTCTAGGCAAGATGACAA | NGG | 2 | 0.9375 | Tier II |
3 | NC_000081.6 | 15 | 52327976 | + | Slc30a8 | NNNTCTGGGCAAAATGACAG | NGG | 1 | 0.9231 | Tier II |
4 | NC_000067.6 | 1 | 63578051 | - | Adam23 | NNNGCTGGGCAAGATGACAG | NGG | 1 | 0.625 | Tier II |
5 | NC_000080.6 | 14 | 115313838 | + | Gpc5 | NNNTTTGGGCAAAATGACAG | NGG | 2 | 0.5874 | Tier II |
6 | NC_000083.6 | 17 | 24564470 | - | Pkd1 | NNNACAGGGCAAGATGACAG | NGG | 2 | 0.4127 | Tier II |
7 | NC_000069.6 | 3 | 38929011 | + | Fat4 | NNNTTTTGGCAAGATGACAG | NGG | 2 | 0.3636 | Tier II |
8 | NC_000077.6 | 11 | 94590844 | + | Acsf2 | NNNGCTGGGCAAGAAGACAG | NGG | 2 | 0.3618 | Tier II |
9 | NC_000073.6 | 7 | 98144399 | - | Capn5 | NNNACTGGGCAGGATGACAG | NGG | 2 | 0.3439 | Tier II |
10 | NC_000073.6 | 7 | 98144399 | - | Omp | NNNACTGGGCAGGATGACAG | NGG | 2 | 0.3439 | Tier II |
11 | NC_000070.6 | 4 | 36594384 | + | Lingo2 | NNNTCTGGGCAGGGTGACAG | NGG | 2 | 0.337 | Tier II |
12 | NC_000081.6 | 15 | 59471101 | + | Nsmce2 | NNNGCTGGGCAAGATGAAAG | NGG | 2 | 0.3365 | Tier II |
13 | NC_000073.6 | 7 | 112459067 | + | Parva | NNNTCTGGGCAAGAAGAAAG | NGG | 2 | 0.3117 | Tier II |
14 | NC_000075.6 | 9 | 117070592 | + | Rbms3 | NNNTCTGGTCAAGATGACAC | NGG | 2 | 0.2286 | Tier II |
15 | NC_000067.6 | 1 | 127846345 | + | Map3k19 | NNNTCTGAGCAAGCTGACAG | NGG | 2 | 0.2143 | Tier II |
16 | NC_000086.7 | X | 162079554 | - | Nhs | NNNTATGGGCAAGATGACAG | NAG | 2 | 0.1481 | Tier II |
17 | NC_000068.7 | 2 | 163227268 | + | Tox2 | NNNTCTGGGCAAGATGCAAG | NGG | 2 | 0.095 | Tier II |
18 | NC_000068.7 | 2 | 167935444 | + | Ptpn1 | NNNTCTGGGGAAGATGGCAG | NGG | 2 | 0.0686 | Tier II |
19 | NC_000086.7 | X | 79397210 | - | Cfap47 | NNNTCTGGGGAAGATGAGAG | NGG | 2 | 0.0519 | Tier II |
20 | NC_000080.6 | 14 | 28452371 | + | Erc2 | NNNTCTGAGCAAGAGGACAG | NGG | 2 | 0.05 | Tier II |
21 | NC_000070.6 | 4 | 32469986 | + | Bach2 | NNNTCTGGGAAAGAGGACAG | NGG | 2 | 0.0433 | Tier II |
22 | NC_000084.6 | 18 | 78492794 | - | Slc14a2 | NNNTCTGGGCAAGAAGACAG | NGA | 2 | 0.0402 | Tier II |
23 | NC_000071.6 | 5 | 51711439 | + | Ppargc1a | NNNTCTGAGCAAGATGACAG | NTG | 2 | 0.039 | Tier II |
24 | NC_000073.6 | 7 | 128454647 | - | Tial1 | NNNTCTGGGAAAGATGACAG | NTG | 2 | 0.0338 | Tier II |
25 | NC_000085.6 | 19 | 8915874 | + | Ganab | NNNTCTGGGCAAGATGACAG | NGC | 1 | 0.0222 | Tier II |
26 | NC_000070.6 | 4 | 63433245 | - | Whrn | NNNTCTTGGCAAGATGACAG | NGT | 2 | 0.0092 | Tier II |
27 | NC_000071.6 | 5 | 23315559 | - | Gm17434 | NNNTCTGGGCAAGATGACAG | NAC | 2 | 0.0 | Tier II |
28 | NC_000083.6 | 17 | 5136584 | + | Arid1b | NNNTCTGGGGAAGATTACAG | NGG | 2 | 0.0 | Tier II |
29 | NC_000071.6 | 5 | 148593568 | - | Gm46929 | NNNTCTGGCCAAGATAACAG | NGG | 2 | 0.5385 | Tier III |
30 | NC_000077.6 | 11 | 97617329 | - | 2410003L11Rik | NNNTCTGGGCAGGATGACTG | NGG | 2 | 0.3889 | Tier III |
31 | NC_000077.6 | 11 | 97617329 | - | Gm38962 | NNNTCTGGGCAGGATGACTG | NGG | 2 | 0.3889 | Tier III |
32 | NC_000073.6 | 7 | 112459067 | + | Gm45998 | NNNTCTGGGCAAGAAGAAAG | NGG | 2 | 0.3117 | Tier III |
33 | NC_000068.7 | 2 | 25478344 | + | Gm35287 | NNNGCTGGGCAAGATGACAG | NAG | 2 | 0.162 | Tier III |
34 | NC_000071.6 | 5 | 29719459 | + | Gm43117 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
35 | NC_000077.6 | 11 | 60561058 | + | Gm12628 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
36 | NC_000077.6 | 11 | 60591855 | + | Gm12621 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
37 | NC_000077.6 | 11 | 60601061 | + | Gm12621 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
38 | NC_000077.6 | 11 | 60610270 | + | Gm12614 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
39 | NC_000077.6 | 11 | 60619480 | + | Gm12614 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
40 | NC_000077.6 | 11 | 60628716 | + | Gm12625 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
41 | NC_000077.6 | 11 | 60641995 | + | Gm12625 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
42 | NC_000077.6 | 11 | 60651204 | + | Gm12619 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
43 | NC_000077.6 | 11 | 60666818 | + | Gm12624 | NNNTCTGGGCAAGCTGACAC | NGG | 2 | 0.0918 | Tier III |
44 | NC_000079.6 | 13 | 23798846 | + | Gm11337 | NNNTCTGGGCAACATGGCAG | NGG | 2 | 0.0743 | Tier III |
45 | NC_000071.6 | 5 | 27953109 | - | Gm17695 | NNNTCTGGGCAAGATGACAG | NAC | 2 | 0.0 | Tier III |