Construct: sgRNA BRDN0001149491

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATTCTGGGCAAGATGACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K7 (5609)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77822

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 7910796 + MAP2K7 NNNTCTGGGCAAGATGACAG NGG 0 1.0 Tier I
2 NC_000020.11 20 20614613 + RALGAPA2 NNNTCTGGGAAAGATGACAT NGG 2 0.6067 Tier II
3 NC_000016.10 16 83459201 - CDH13 NNNTCTGGACAAAATGACAG NGG 2 0.5934 Tier II
4 NC_000005.10 5 167249080 + TENM2 NNNTCTTGGCAAGATGACAG NGG 1 0.5714 Tier II
5 NC_000023.11 X 49189948 + SYP NNNTCTGGGAGAGATGACAG NGG 2 0.5633 Tier II
6 NC_000016.10 16 12174384 + SNX29 NNNTCAGGGCAAGAAGACAG NGG 2 0.5018 Tier II
7 NC_000019.10 19 7910886 + MAP2K7 NNNACTGGGCAAGATGACAG NGG 1 0.4762 Tier II
8 NC_000011.10 11 66605075 - CCS NNNCCTGGGCAGGATGACAG NGG 2 0.4673 Tier II
9 NC_000005.10 5 172899072 + ERGIC1 NNNTCTGAGCAAGGTGACAG NGG 2 0.4667 Tier II
10 NC_000018.10 18 21422254 + GREB1L NNNTCTGAGCAAGGTGACAG NGG 2 0.4667 Tier II
11 NC_000019.10 19 23864166 + RPSA2 NNNTCTGAGCAAGATGACAC NGG 2 0.4286 Tier II
12 NC_000007.14 7 45174294 + RAMP3 NNNTCTGGGACAGATGACAG NGG 2 0.3467 Tier II
13 NC_000019.10 19 7910841 + MAP2K7 NNNACTGGGCAGGATGACAG NGG 2 0.3439 Tier II
14 NC_000015.10 15 74311682 + CCDC33 NNNTCTGGGTTAGATGACAG NGG 2 0.2896 Tier II
15 NC_000022.11 22 43592679 + EFCAB6 NNNTCTGGGCAACAAGACAG NGG 2 0.2438 Tier II
16 NC_000015.10 15 41502564 - ITPKA NNNACTGGGCCAGATGACAG NGG 2 0.1905 Tier II
17 NC_000023.11 X 15366816 - VEGFD NNNTCTGGGCTAGAAGACAG NGG 2 0.1781 Tier II
18 NC_000010.11 10 80521553 - TSPAN14 NNNCCTGGGCACGATGACAG NGG 2 0.1703 Tier II
19 NC_000012.12 12 51192151 - POU6F1 NNNTTTGGGCACGATGACAG NGG 2 0.1675 Tier II
20 NC_000008.11 8 23530066 - SLC25A37 NNNTCTGGGCAGGCTGACAG NGG 2 0.1548 Tier II
21 NC_000023.11 X 57454093 - FAAH2 NNNTCTGGCCAAGATGACAG NAG 2 0.1396 Tier II
22 NC_000004.12 4 2423696 - CFAP99 NNNCCTGGGCAAGATGAGAG NGG 2 0.0863 Tier II
23 NC_000016.10 16 48334607 + LONP2 NNNTCTGGGCAAGGTGGCAG NGG 2 0.0824 Tier II
24 NC_000003.12 3 57466518 + DNAH12 NNNTCTGGGCATGCTGACAG NGG 2 0.0714 Tier II
25 NC_000001.11 1 3110974 + PRDM16 NNNTCTGGGCAAGACGGCAG NGG 2 0.0481 Tier II
26 NC_000019.10 19 35708103 + ZBTB32 NNNCCTGGGCAAGATGACAG NGA 2 0.0449 Tier II
27 NC_000015.10 15 72291053 - CELF6 NNNTCAGGGCAAGAGGACAG NGG 2 0.0433 Tier II
28 NC_000007.14 7 44240827 - CAMK2B NNNTCTGGGCAGGAGGACAG NGG 2 0.0361 Tier II
29 NC_000011.10 11 96125531 + MAML2 NNNTCTGGGCAAGTGGACAG NGG 2 0.0267 Tier II
30 NC_000005.10 5 119633309 + FAM170A NNNTCTGGGCAAGATGACAG NGC 1 0.0222 Tier II
31 NC_000009.12 9 117246652 + ASTN2 NNNTCTGGGGAAGAGGACAG NGG 2 0.0194 Tier II
32 NC_000002.12 2 172786851 - RAPGEF4 NNNTCTGGGCTAGAGGACAG NGG 2 0.0154 Tier II
33 NC_000003.12 3 165157838 - SI NNNTCTGGGCTAGAGGACAG NGG 2 0.0154 Tier II
34 NC_000008.11 8 22982220 + RHOBTB2 NNNCCTGGGCAAGATGACAG NGT 2 0.0104 Tier II
35 NC_000003.12 3 10273645 + TATDN2 NNNTCTGGGCAAGATGATAG NGT 2 0.0104 Tier II
36 NC_000016.10 16 70494007 - COG4 NNNTCTGGGCAAGATGAGAG NGA 2 0.0093 Tier II
37 NC_000011.10 11 19647796 + NAV2 NNNTCTGGGCAAGAGGGCAG NGG 2 0.0088 Tier II
38 NC_000001.11 1 169820149 + FIRRM NNNTCTGGGCAAGATGAAAG NGT 2 0.0087 Tier II
39 NC_000001.11 1 151806263 + RORC NNNTCTGGGCAAGATCACAG NTG 2 0.0 Tier II
40 NC_000022.11 22 38868532 + CBX6 NNNTCTGGGCAAGATCCCAG NGG 2 0.0 Tier II
41 NC_000016.10 16 22210939 + EEF2K NNNTCTGGGCAGGATCACAG NGG 2 0.0 Tier II
42 NC_000003.12 3 954567 + LOC107986059 NNNTCTGGGCAAGAAAACAG NGG 2 0.5789 Tier III
43 NC_000018.10 18 21422254 + LOC101927521 NNNTCTGAGCAAGGTGACAG NGG 2 0.4667 Tier III
44 NC_000015.10 15 74311682 + LOC124903525 NNNTCTGGGTTAGATGACAG NGG 2 0.2896 Tier III
45 NC_000013.11 13 22220593 + LINC00540 NNNACTGGGCCAGATGACAG NGG 2 0.1905 Tier III
46 NC_000023.11 X 15366816 - PIR-FIGF NNNTCTGGGCTAGAAGACAG NGG 2 0.1781 Tier III
47 NC_000002.12 2 108330402 - SULT1C5P NNNTCTTGGCAAGCTGACAG NGG 2 0.1224 Tier III
48 NC_000016.10 16 55654298 - LOC124903693 NNNTCTGGGGAAGATGACAG NAG 2 0.1008 Tier III
49 NC_000016.10 16 48334607 + UBA52P8 NNNTCTGGGCAAGGTGGCAG NGG 2 0.0824 Tier III
50 NC_000013.11 13 53539146 + LOC105370210 NNNTCTGGGCTAGATGCCAG NGG 2 0.0543 Tier III
51 NC_000007.14 7 25689716 + UBA52P1 NNNTCTGGGCAAGCTGGCAG NGG 2 0.0378 Tier III
52 NC_000007.14 7 25689716 + LINC03007 NNNTCTGGGCAAGCTGGCAG NGG 2 0.0378 Tier III
53 NC_000002.12 2 85934698 - LOC124906031 NNNTCTGTGCAAGATGACAG NTG 2 0.0244 Tier III
54 NC_000008.11 8 47062951 - LOC105375815 NNNTCTGGGCAAGATGAAAG NGC 2 0.012 Tier III
55 NC_000008.11 8 22982220 + LOC107984124 NNNCCTGGGCAAGATGACAG NGT 2 0.0104 Tier III
56 NC_000006.12 6 123668946 + LOC105377981 NNNTCTGGGCAAGTTCACAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 4244329 + Map2k7 NNNCCTGGGCAAGATGACTG NGG 2 0.3484 Tier I
2 NC_000068.7 2 167471212 - Slc9a8 NNNTCTAGGCAAGATGACAA NGG 2 0.9375 Tier II
3 NC_000081.6 15 52327976 + Slc30a8 NNNTCTGGGCAAAATGACAG NGG 1 0.9231 Tier II
4 NC_000067.6 1 63578051 - Adam23 NNNGCTGGGCAAGATGACAG NGG 1 0.625 Tier II
5 NC_000080.6 14 115313838 + Gpc5 NNNTTTGGGCAAAATGACAG NGG 2 0.5874 Tier II
6 NC_000083.6 17 24564470 - Pkd1 NNNACAGGGCAAGATGACAG NGG 2 0.4127 Tier II
7 NC_000069.6 3 38929011 + Fat4 NNNTTTTGGCAAGATGACAG NGG 2 0.3636 Tier II
8 NC_000077.6 11 94590844 + Acsf2 NNNGCTGGGCAAGAAGACAG NGG 2 0.3618 Tier II
9 NC_000073.6 7 98144399 - Capn5 NNNACTGGGCAGGATGACAG NGG 2 0.3439 Tier II
10 NC_000073.6 7 98144399 - Omp NNNACTGGGCAGGATGACAG NGG 2 0.3439 Tier II
11 NC_000070.6 4 36594384 + Lingo2 NNNTCTGGGCAGGGTGACAG NGG 2 0.337 Tier II
12 NC_000081.6 15 59471101 + Nsmce2 NNNGCTGGGCAAGATGAAAG NGG 2 0.3365 Tier II
13 NC_000073.6 7 112459067 + Parva NNNTCTGGGCAAGAAGAAAG NGG 2 0.3117 Tier II
14 NC_000075.6 9 117070592 + Rbms3 NNNTCTGGTCAAGATGACAC NGG 2 0.2286 Tier II
15 NC_000067.6 1 127846345 + Map3k19 NNNTCTGAGCAAGCTGACAG NGG 2 0.2143 Tier II
16 NC_000086.7 X 162079554 - Nhs NNNTATGGGCAAGATGACAG NAG 2 0.1481 Tier II
17 NC_000068.7 2 163227268 + Tox2 NNNTCTGGGCAAGATGCAAG NGG 2 0.095 Tier II
18 NC_000068.7 2 167935444 + Ptpn1 NNNTCTGGGGAAGATGGCAG NGG 2 0.0686 Tier II
19 NC_000086.7 X 79397210 - Cfap47 NNNTCTGGGGAAGATGAGAG NGG 2 0.0519 Tier II
20 NC_000080.6 14 28452371 + Erc2 NNNTCTGAGCAAGAGGACAG NGG 2 0.05 Tier II
21 NC_000070.6 4 32469986 + Bach2 NNNTCTGGGAAAGAGGACAG NGG 2 0.0433 Tier II
22 NC_000084.6 18 78492794 - Slc14a2 NNNTCTGGGCAAGAAGACAG NGA 2 0.0402 Tier II
23 NC_000071.6 5 51711439 + Ppargc1a NNNTCTGAGCAAGATGACAG NTG 2 0.039 Tier II
24 NC_000073.6 7 128454647 - Tial1 NNNTCTGGGAAAGATGACAG NTG 2 0.0338 Tier II
25 NC_000085.6 19 8915874 + Ganab NNNTCTGGGCAAGATGACAG NGC 1 0.0222 Tier II
26 NC_000070.6 4 63433245 - Whrn NNNTCTTGGCAAGATGACAG NGT 2 0.0092 Tier II
27 NC_000071.6 5 23315559 - Gm17434 NNNTCTGGGCAAGATGACAG NAC 2 0.0 Tier II
28 NC_000083.6 17 5136584 + Arid1b NNNTCTGGGGAAGATTACAG NGG 2 0.0 Tier II
29 NC_000071.6 5 148593568 - Gm46929 NNNTCTGGCCAAGATAACAG NGG 2 0.5385 Tier III
30 NC_000077.6 11 97617329 - 2410003L11Rik NNNTCTGGGCAGGATGACTG NGG 2 0.3889 Tier III
31 NC_000077.6 11 97617329 - Gm38962 NNNTCTGGGCAGGATGACTG NGG 2 0.3889 Tier III
32 NC_000073.6 7 112459067 + Gm45998 NNNTCTGGGCAAGAAGAAAG NGG 2 0.3117 Tier III
33 NC_000068.7 2 25478344 + Gm35287 NNNGCTGGGCAAGATGACAG NAG 2 0.162 Tier III
34 NC_000071.6 5 29719459 + Gm43117 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
35 NC_000077.6 11 60561058 + Gm12628 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
36 NC_000077.6 11 60591855 + Gm12621 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
37 NC_000077.6 11 60601061 + Gm12621 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
38 NC_000077.6 11 60610270 + Gm12614 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
39 NC_000077.6 11 60619480 + Gm12614 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
40 NC_000077.6 11 60628716 + Gm12625 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
41 NC_000077.6 11 60641995 + Gm12625 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
42 NC_000077.6 11 60651204 + Gm12619 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
43 NC_000077.6 11 60666818 + Gm12624 NNNTCTGGGCAAGCTGACAC NGG 2 0.0918 Tier III
44 NC_000079.6 13 23798846 + Gm11337 NNNTCTGGGCAACATGGCAG NGG 2 0.0743 Tier III
45 NC_000071.6 5 27953109 - Gm17695 NNNTCTGGGCAAGATGACAG NAC 2 0.0 Tier III
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Other clones with same target sequence:

(none)