Construct: sgRNA BRDN0001149503

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTATCATAGGAACATCGTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK8 (158067)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77883

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 132826932 - AK8 NNNTCATAGGAACATCGTCA NGG 0 1.0 Tier I
2 NC_000017.11 17 30084366 + EFCAB5 NNNTCAGAAGAACATCGTCA NGG 2 0.3782 Tier II
3 NC_000014.9 14 32343064 - AKAP6 NNNTCATGGGAAAATCGTCA NGG 2 0.2821 Tier II
4 NC_000023.11 X 112668571 - LHFPL1 NNNTCATAGGAACATAGTCA NAG 2 0.2593 Tier II
5 NC_000002.12 2 45660319 - PRKCE NNNTCATAAGAACATCTTCA NGG 2 0.1607 Tier II
6 NC_000014.9 14 58087375 + ARMH4 NNNACATAGGAACATCCTCA NGG 2 0.112 Tier II
7 NC_000013.11 13 73701136 - KLF12 NNNTCATAGGAACATTTTCA NGG 2 0.0769 Tier II
8 NC_000009.12 9 122308046 + MRRF NNNTCATAGGAACATGCTCA NGG 2 0.0362 Tier II
9 NC_000001.11 1 219922581 - SLC30A10 NNNTCATAGCAACAGCGTCA NGG 2 0.02 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 80128837 - Pde1a NNNTCCTAGGAACATCATCA NGG 2 0.4242 Tier I
2 NC_000084.6 18 35299035 + Sil1 NNNTCCTAAGAACATCGTCA NGG 2 0.2922 Tier II
3 NC_000080.6 14 117661637 + Gpc6 NNNCCATAGGAACATCCTCA NGG 2 0.1522 Tier II
4 NC_000068.7 2 118444319 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
5 NC_000068.7 2 118444385 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
6 NC_000068.7 2 118444451 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
7 NC_000068.7 2 118444549 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
8 NC_000068.7 2 118444583 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
9 NC_000068.7 2 118444649 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
10 NC_000068.7 2 118444715 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
11 NC_000068.7 2 118444749 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
12 NC_000068.7 2 118444815 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
13 NC_000068.7 2 118444849 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
14 NC_000068.7 2 118444947 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
15 NC_000068.7 2 118444981 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
16 NC_000068.7 2 118445015 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
17 NC_000068.7 2 118445113 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
18 NC_000068.7 2 118445147 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
19 NC_000068.7 2 118445245 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
20 NC_000068.7 2 118445279 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
21 NC_000068.7 2 118445379 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
22 NC_000068.7 2 118445413 - Eif2ak4 NNNTCATAGGAACATCCTCA NTG 2 0.0092 Tier II
23 NC_000079.6 13 95812673 - Iqgap2 NNNTCATAGGATCATCGTCA NGC 2 0.0074 Tier II
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Other clones with same target sequence:

(none)