Construct: sgRNA BRDN0001149509

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGCTGAAGTCATGAGCACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK40 (83931)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75628

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 36355342 + STK40 NNNTGAAGTCATGAGCACAG NGG 0 1.0 Tier I
2 NC_000003.12 3 27394541 + SLC4A7 NNNTGAAGTCATGAACACAG NGA 2 0.0654 Tier I
3 NC_000004.12 4 155905635 - TDO2 NNNTGAAGTCATGAACACAT NGG 2 0.6588 Tier II
4 NC_000011.10 11 68743722 - TESMIN NNNTGAACTCATGAGCACAG NGG 1 0.6154 Tier II
5 NC_000004.12 4 115013024 + NDST4 NNNTGAACTCATGAACACAG NGG 2 0.5792 Tier II
6 NC_000001.11 1 200995511 + KIF21B NNNTGAAGTCAGAAGCACAG NGG 2 0.4615 Tier II
7 NC_000011.10 11 1015720 + MUC6 NNNGGGAGTCATGAGCACAG NGG 2 0.4464 Tier II
8 NC_000005.10 5 59825969 - PDE4D NNNAGAAGCCATGAGCACAG NGG 2 0.4396 Tier II
9 NC_000010.11 10 7745074 + ITIH2 NNNTGGACTCATGAGCACAG NGG 2 0.4396 Tier II
10 NC_000021.9 21 32210607 + MIS18A NNNTGATGTCATGAGCACAA NGG 2 0.4102 Tier II
11 NC_000011.10 11 75495855 - GDPD5 NNNTGAAGTCACCAGCACAG NGG 2 0.3989 Tier II
12 NC_000019.10 19 30339345 + ZNF536 NNNTGAAGCCCTGAGCACAG NGG 2 0.3692 Tier II
13 NC_000010.11 10 119423763 + GRK5 NNNTGAAGGCATGAGCACTG NGG 2 0.3333 Tier II
14 NC_000004.12 4 37292313 + NWD2 NNNTTAAGTCATGAGAACAG NGG 2 0.3 Tier II
15 NC_000013.11 13 107379630 - NALF1 NNNTGGAGTCCTGAGCACAG NGG 2 0.2857 Tier II
16 NC_000008.11 8 13385819 + DLC1 NNNTTAAGACATGAGCACAG NGG 2 0.2786 Tier II
17 NC_000011.10 11 133081132 - OPCML NNNTGAAGTCAGGAGCACAC NGG 2 0.2143 Tier II
18 NC_000016.10 16 25965281 + HS3ST4 NNNTGAAGCCATGAGCAGAG NGG 2 0.1231 Tier II
19 NC_000011.10 11 75173177 - SLCO2B1 NNNTTAAGTGATGAGCACAG NGG 2 0.1167 Tier II
20 NC_000001.11 1 213146076 - RPS6KC1 NNNTGAATTCATGAGCACAG NGA 2 0.0434 Tier II
21 NC_000003.12 3 108838262 + TRAT1 NNNTGAAGTCATGATCACAG NAG 2 0.037 Tier II
22 NC_000010.11 10 80201385 - ANXA11 NNNTGAAGTCATGGGCACAG NGA 2 0.0324 Tier II
23 NC_000006.12 6 38892248 - DNAH8 NNNTGAAGTCATCAGCACAG NGA 2 0.0292 Tier II
24 NC_000010.11 10 45767389 - WASHC2C NNNTGAAGGCATGAGCACAG NTG 2 0.0241 Tier II
25 NC_000010.11 10 50108044 - WASHC2A NNNTGAAGGCATGAGCACAG NTG 2 0.0241 Tier II
26 NC_000008.11 8 123883854 - FER1L6 NNNTGAAGTCATGAGAACAG NGC 2 0.0222 Tier II
27 NC_000015.10 15 76356248 + SCAPER NNNTGATGTCATGAGCACAG NTG 2 0.017 Tier II
28 NC_000016.10 16 14691385 - PLA2G10 NNNGGAAGTCATGAGCACAG NGC 2 0.0139 Tier II
29 NC_000016.10 16 14730502 - LOC100652777 NNNGGAAGTCATGAGCACAG NGC 2 0.0139 Tier II
30 NC_000012.12 12 100291899 - SCYL2 NNNTGTAGTCATGAGCACAG NGT 2 0.0115 Tier II
31 NC_000001.11 1 162675839 + DDR2 NNNTGAAGTCATGAGCACAG NCA 2 0.0 Tier II
32 NC_000017.11 17 12631577 + LINC00670 NNNTGAGGTAATGAGCACAG NGG 2 0.6118 Tier III
33 NC_000004.12 4 115013024 + LOC124900764 NNNTGAACTCATGAACACAG NGG 2 0.5792 Tier III
34 NC_000005.10 5 25977690 - LOC124901176 NNNTGAAATCATGAGCACTG NGG 2 0.5385 Tier III
35 NC_000003.12 3 67805959 + SUCLG2-DT NNNTGCAGTCATGAGAACAG NGG 2 0.4545 Tier III
36 NC_000004.12 4 186978426 + LOC102723906 NNNTGCAGTCATGAGCACAG NGG 1 0.4545 Tier III
37 NC_000015.10 15 40311151 + LOC112268152 NNNTGAAGTGATGAGCACAA NGG 2 0.3646 Tier III
38 NC_000018.10 18 49627316 + LOC105372112 NNNGGAAGTCATGTGCACAG NGG 2 0.3333 Tier III
39 NC_000011.10 11 87437515 + LOC107984361 NNNTTAAGTCATGAGCACTG NGG 2 0.1615 Tier III
40 NC_000002.12 2 220835834 - LOC107985990 NNNTGAAGTCATGGGTACAG NGG 2 0.1436 Tier III
41 NC_000016.10 16 35452006 + LOC105371203 NNNAGAAGTCATGAGCCCAG NGG 2 0.084 Tier III
42 NC_000009.12 9 93824998 - LOC101928014 NNNTGAAGACATGAGCACAG NTG 2 0.0362 Tier III
43 NC_000001.11 1 67955365 + GNG12-AS1 NNNTGAAGTCATGAGCACAC NTG 2 0.0167 Tier III
44 NC_000001.11 1 67955365 + LOC124900403 NNNTGAAGTCATGAGCACAC NTG 2 0.0167 Tier III
45 NC_000016.10 16 29058201 - PLA2G10CP NNNGGAAGTCATGAGCACAG NGC 2 0.0139 Tier III
46 NC_000016.10 16 30217479 + LOC101929894 NNNGGAAGTCATGAGCACAG NGC 2 0.0139 Tier III
47 NC_000008.11 8 128027305 - PVT1 NNNTGAAGTCCTGAGCACAG NGC 2 0.0089 Tier III
48 NC_000001.11 1 112358210 - LINC02884 NNNTGAAGTCATTAGCACAG NGC 2 0.0067 Tier III
49 NC_000014.9 14 49527300 + LOC107984701 NNNTGAAGTCATGAGCAGAG NTG 2 0.0052 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 126128911 - Stk40 NNNTGAAGTCGTGTGCACAG NGG 2 0.3467 Tier I
2 NC_000080.6 14 14775406 - Slc4a7 NNNTGAAGTCATGAACACAG NGA 2 0.0654 Tier I
3 NC_000073.6 7 119478390 + Umod NNNTGAAATCATGAGAACAG NGG 2 1.0 Tier II
4 NC_000067.6 1 22560984 + Rims1 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
5 NC_000067.6 1 175236938 + Rgs7 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
6 NC_000067.6 1 178138639 + Catspere2 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
7 NC_000068.7 2 41309738 + Lrp1b NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
8 NC_000068.7 2 62865732 + Kcnh7 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
9 NC_000068.7 2 97241668 - Lrrc4c NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
10 NC_000070.6 4 78165831 + Ptprd NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
11 NC_000071.6 5 21284894 + Gsap NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
12 NC_000072.6 6 57614316 + Herc6 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
13 NC_000074.6 8 58466878 - Galntl6 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
14 NC_000076.6 10 36642847 - Hs3st5 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
15 NC_000076.6 10 68762286 - Tmem26 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
16 NC_000076.6 10 68764427 - Tmem26 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
17 NC_000078.6 12 101597362 - Catsperb NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
18 NC_000083.6 17 57390809 - Adgre1 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
19 NC_000084.6 18 16705957 - Cdh2 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
20 NC_000086.7 X 6505441 - Shroom4 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier II
21 NC_000075.6 9 92353355 - 1700057G04Rik NNNGGAAGTCATGAGAACAG NGG 2 0.625 Tier II
22 NC_000073.6 7 76803381 + Agbl1 NNNTGAACTCATGAGCACAG NGG 1 0.6154 Tier II
23 NC_000070.6 4 19502247 - Cngb3 NNNCGAAGACATGAGCACAG NGG 2 0.6008 Tier II
24 NC_000067.6 1 39692684 - Rfx8 NNNTGAACTCATGAGCACAA NGG 2 0.5769 Tier II
25 NC_000068.7 2 76160383 - Pde11a NNNTGAACACATGAGCACAG NGG 2 0.5714 Tier II
26 NC_000071.6 5 107456805 + Btbd8 NNNTGAACACATGAGCACAG NGG 2 0.5714 Tier II
27 NC_000073.6 7 87659348 - Grm5 NNNTGAACACATGAGCACAG NGG 2 0.5714 Tier II
28 NC_000073.6 7 90662692 - Dlg2 NNNTGAACACATGAGCACAG NGG 2 0.5714 Tier II
29 NC_000074.6 8 17188547 + Csmd1 NNNTGAACACATGAGCACAG NGG 2 0.5714 Tier II
30 NC_000070.6 4 85749982 + Adamtsl1 NNNTGAAGGAATGAGCACAG NGG 2 0.5365 Tier II
31 NC_000071.6 5 98420867 - Cfap299 NNNTGAAGTCATGGGCACAG NGG 1 0.4667 Tier II
32 NC_000070.6 4 17871308 - Mmp16 NNNTGAAGTTATGGGCACAG NGG 2 0.4392 Tier II
33 NC_000079.6 13 117623818 - Hcn1 NNNTGAAGTTATGGGCACAG NGG 2 0.4392 Tier II
34 NC_000085.6 19 48612329 + Sorcs3 NNNTGAAGTTATGGGCACAG NGG 2 0.4392 Tier II
35 NC_000071.6 5 14575888 + Pclo NNNTGAGGGCATGAGCACAG NGG 2 0.437 Tier II
36 NC_000071.6 5 150148764 + Fry NNNTGAGCTCATGAGCACAG NGG 2 0.4344 Tier II
37 NC_000068.7 2 105850257 + Elp4 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
38 NC_000069.6 3 55941595 + Nbea NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
39 NC_000069.6 3 106728567 - Lrif1 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
40 NC_000069.6 3 139055131 - Rap1gds1 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
41 NC_000070.6 4 112962165 + Skint6 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
42 NC_000072.6 6 23670215 - Cadps2 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
43 NC_000073.6 7 90579635 + Dlg2 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
44 NC_000074.6 8 47911760 - Wwc2 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
45 NC_000081.6 15 28432508 + Dnah5 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
46 NC_000085.6 19 36423808 - Pcgf5 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
47 NC_000085.6 19 55437981 + Vti1a NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
48 NC_000086.7 X 98610414 + Ophn1 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier II
49 NC_000083.6 17 24192809 + Tbc1d24 NNNTGAAGCCATGAGCACAC NGG 2 0.3956 Tier II
50 NC_000074.6 8 28666262 + Unc5d NNNTGAAGTCATGAGCACGG NGG 1 0.375 Tier II
51 NC_000075.6 9 98359730 + Nmnat3 NNNTGCAGTCAAGAGCACAG NGG 2 0.3636 Tier II
52 NC_000076.6 10 89508767 - Nr1h4 NNNTGAGGTCAGGAGCACAG NGG 2 0.3529 Tier II
53 NC_000068.7 2 178254422 - Phactr3 NNNTGAATTCATGTGCACAG NGG 2 0.3333 Tier II
54 NC_000068.7 2 33096205 - Garnl3 NNNTGGTGTCATGAGCACAG NGG 2 0.3125 Tier II
55 NC_000068.7 2 37477652 - Rabgap1 NNNTGAACTCATGGGCACAG NGG 2 0.2872 Tier II
56 NC_000075.6 9 30956325 - Adamts8 NNNTGACTTCATGAGCACAG NGG 2 0.2734 Tier II
57 NC_000073.6 7 76611421 + Agbl1 NNNTGAAATCATGAGCACAG NAG 2 0.2593 Tier II
58 NC_000086.7 X 157894328 + Cnksr2 NNNTGAACTCATCAGCACAG NGG 2 0.2591 Tier II
59 NC_000073.6 7 98457447 - Gucy2d NNNTGAACTCCTGAGCACAG NGG 2 0.2462 Tier II
60 NC_000067.6 1 70074737 - Spag16 NNNTGAAGTCATAAGCACAG NAG 2 0.2393 Tier II
61 NC_000072.6 6 118709194 - Cacna1c NNNTGAAGTCAGGAGCACAC NGG 2 0.2143 Tier II
62 NC_000083.6 17 10938644 - Prkn NNNAGAAGTCATGAGCACAC NGG 2 0.2041 Tier II
63 NC_000071.6 5 19772615 + Magi2 NNNTGAACTCATTAGCACAG NGG 2 0.1846 Tier II
64 NC_000076.6 10 32551719 + Nkain2 NNNTGAACTCATTAGCACAG NGG 2 0.1846 Tier II
65 NC_000083.6 17 20211850 + Vmn2r105 NNNTGAACTCATTAGCACAG NGG 2 0.1846 Tier II
66 NC_000084.6 18 12339819 - Lama3 NNNTGAACTCATTAGCACAG NGG 2 0.1846 Tier II
67 NC_000086.7 X 56076420 + 3830403N18Rik NNNTGAACTCATTAGCACAG NGG 2 0.1846 Tier II
68 NC_000081.6 15 9622542 + Spef2 NNNTGAAGTCATGAGCATAG NAG 2 0.1667 Tier II
69 NC_000073.6 7 46602602 - Sergef NNNTGAAGTCATGTCCACAG NGG 2 0.1455 Tier II
70 NC_000070.6 4 51227907 - Cylc2 NNNTGAAGTCATGGGTACAG NGG 2 0.1436 Tier II
71 NC_000079.6 13 77442562 - 2210408I21Rik NNNTGAAGTCATTGGCACAG NGG 2 0.14 Tier II
72 NC_000083.6 17 18096240 - Vmn2r91 NNNTGAAGTCATTGGCACAG NGG 2 0.14 Tier II
73 NC_000085.6 19 47236244 + Neurl1a NNNTGAAGTCATCACCACAG NGG 2 0.1148 Tier II
74 NC_000070.6 4 84600002 + Bnc2 NNNTGAATTCATGAGCGCAG NGG 2 0.1103 Tier II
75 NC_000076.6 10 73368151 + Pcdh15 NNNTGAGGTCATGAGGACAG NGG 2 0.1086 Tier II
76 NC_000077.6 11 115883152 + Myo15b NNNGGAAGTCATGAGGACAG NGG 2 0.0962 Tier II
77 NC_000075.6 9 43761161 + Nectin1 NNNTGAAGGCATGAGGACAG NGG 2 0.0952 Tier II
78 NC_000084.6 18 47066554 - Commd10 NNNTGAAGGCATGAGGACAG NGG 2 0.0952 Tier II
79 NC_000083.6 17 25434635 + Cacna1h NNNTGAAGGCATGAGCAGAG NGG 2 0.0825 Tier II
80 NC_000067.6 1 178110522 - Catspere2 NNNTGAAGTCATGAGCACAG NGC 1 0.0222 Tier II
81 NC_000083.6 17 49846184 - Kif6 NNNTGAAGTCATGAGCACAT NGC 2 0.0156 Tier II
82 NC_000067.6 1 177879115 - Catspere1 NNNTGAAGTCATGAGCATAG NGC 2 0.0143 Tier II
83 NC_000083.6 17 90308018 + Nrxn1 NNNTGAAGTCATGAGCACAC NGT 2 0.0069 Tier II
84 NC_000073.6 7 3339341 + Cacng7 NNNTGAAGTCATGAGCTCAG NGC 2 0.003 Tier II
85 NC_000067.6 1 150342713 + 2310030A07Rik NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
86 NC_000067.6 1 163547548 + Gm32333 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
87 NC_000068.7 2 41309738 + Gm30138 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
88 NC_000070.6 4 24124729 + Gm27243 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
89 NC_000073.6 7 42848339 + Gm2381 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
90 NC_000077.6 11 40160498 + Gm12132 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
91 NC_000079.6 13 19330217 + Tcrg NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
92 NC_000079.6 13 102473263 + Gm41045 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
93 NC_000080.6 14 18516818 - Gm32587 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
94 NC_000086.7 X 4335084 - Gm14362 NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
95 NC_000086.7 X 6505441 - 5430428K19Rik NNNTGAAGATATGAGCACAG NGG 2 0.8739 Tier III
96 NC_000079.6 13 78543841 - Gm32067 NNNTGAACACATGAGCACAG NGG 2 0.5714 Tier III
97 NC_000069.6 3 84062580 - Gm30458 NNNTGAAGTCAGGAGAACAG NGG 2 0.5 Tier III
98 NC_000086.7 X 95689689 - Gm29889 NNNTGAAGTTATGGGCACAG NGG 2 0.4392 Tier III
99 NC_000069.6 3 55941595 + Gm31914 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier III
100 NC_000074.6 8 71817916 + Gm40504 NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier III
101 NC_000078.6 12 114849519 + Igh NNNTGAAGACATGGGCACAG NGG 2 0.4333 Tier III
102 NC_000070.6 4 24180968 - Gm27243 NNNTGAAGTTATCAGCACAG NGG 2 0.3963 Tier III
103 NC_000072.6 6 48162168 - Gm16630 NNNTGAAGCCATCAGCACAG NGG 2 0.3887 Tier III
104 NC_000081.6 15 84547163 - Gm34027 NNNTGAATTCAGGAGCACAG NGG 2 0.3125 Tier III
105 NC_000081.6 15 46465645 + 4930523O13Rik NNNTGAAATCTTGAGCACAG NGG 2 0.3077 Tier III
106 NC_000078.6 12 114860299 - Igh NNNCGAAGTCATGGGCACAG NGG 2 0.302 Tier III
107 NC_000080.6 14 23070099 - Gm10248 NNNTGAACTCATGGGCACAG NGG 2 0.2872 Tier III
108 NC_000071.6 5 88141291 + Gm42622 NNNTGAACTCATCAGCACAG NGG 2 0.2591 Tier III
109 NC_000068.7 2 117860842 - 4930412B13Rik NNNTGAAGTCAAGACCACAG NGG 2 0.2182 Tier III
110 NC_000068.7 2 117860842 - Gm13986 NNNTGAAGTCAAGACCACAG NGG 2 0.2182 Tier III
111 NC_000071.6 5 19772615 + Gm52765 NNNTGAACTCATTAGCACAG NGG 2 0.1846 Tier III
112 NC_000078.6 12 50208441 + Gm34523 NNNTGAACTCATGAGCACAG NAG 2 0.1595 Tier III
113 NC_000069.6 3 75172748 - Gm40071 NNNTGAAGTCATTGGCACAG NGG 2 0.14 Tier III
114 NC_000068.7 2 46430567 + Gm13470 NNNTGCAGTCATGAGTACAG NGG 2 0.1399 Tier III
115 NC_000075.6 9 43761161 + Gm46108 NNNTGAAGGCATGAGGACAG NGG 2 0.0952 Tier III
116 NC_000078.6 12 13107036 - Gm35298 NNNTGAAGTCCTGAGCACAG NTG 2 0.0156 Tier III
117 NC_000076.6 10 27654339 + Gm30165 NNNTGAAGTCATGAGTACAG NGT 2 0.005 Tier III
Download CSV

Other clones with same target sequence:

(none)