Construct: sgRNA BRDN0001149513
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGAAGCGGGCGAGGACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKA (3706)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76857
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
41494407 |
+ |
ITPKA |
NNNGAAGCGGGCGAGGACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
22207391 |
- |
BMP1 |
NNNGAAGCGGCCGAGGACGG |
NGG |
2 |
0.0756 |
Tier I |
| 3 |
NC_000016.10 |
16 |
4332360 |
+ |
GLIS2 |
NNNGAAGCGGGAGAGGACGC |
NGG |
2 |
0.0649 |
Tier I |
| 4 |
NC_000011.10 |
11 |
1181822 |
- |
MUC5AC |
NNNGAAGAGGGCGAGGACAT |
NGG |
2 |
0.4643 |
Tier II |
| 5 |
NC_000009.12 |
9 |
133942314 |
+ |
VAV2 |
NNNGAAGCGGGAGAGGGCGT |
NGG |
2 |
0.1261 |
Tier II |
| 6 |
NC_000009.12 |
9 |
131096743 |
+ |
AIF1L |
NNNGAAGCGGGCGGGGACGG |
NGG |
2 |
0.0824 |
Tier II |
| 7 |
NC_000008.11 |
8 |
141442156 |
+ |
MROH5 |
NNNGCAGCGGGCGAGGGCGT |
NGG |
2 |
0.0882 |
Tier III |
| 8 |
NC_000008.11 |
8 |
141442156 |
+ |
LOC105375789 |
NNNGCAGCGGGCGAGGGCGT |
NGG |
2 |
0.0882 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
85478614 |
- |
Fbxo41 |
NNNGCAGCGGGCGAGGCCGT |
NGG |
2 |
0.0882 |
Tier I |
| 2 |
NC_000077.6 |
11 |
117849248 |
+ |
Birc5 |
NNNGCAGCGGGCGAGGGCGT |
NGG |
2 |
0.0882 |
Tier II |
| 3 |
NC_000067.6 |
1 |
70910881 |
- |
Gm29906 |
NNNGAAGCGGGCAAGGAGGT |
NGG |
2 |
0.1231 |
Tier III |
Other clones with same target sequence:
(none)
