Construct: sgRNA BRDN0001149524

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGGAATGAATAATCCAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK5 (57144)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75715

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 9580453 + PAK5 NNNGAATGAATAATCCAGAG NGG 0 1.0 Tier I
2 NC_000009.12 9 32570996 + NDUFB6 NNNTAATGAATAATCCAGAC NGG 2 0.1558 Tier I
3 NC_000004.12 4 167069934 - SPOCK3 NNNGAATGAAAAATCCAGAA NGG 2 0.7031 Tier II
4 NC_000005.10 5 64355288 + RNF180 NNNGAATGAACAATCCAGAA NGG 2 0.625 Tier II
5 NC_000017.11 17 66540454 + PRKCA NNNGGATGAATAATCCAAAG NGG 2 0.4985 Tier II
6 NC_000013.11 13 94490068 + DCT NNNGAATGAATAAACCAGAT NGG 2 0.4333 Tier II
7 NC_000012.12 12 64828619 - TBC1D30 NNNGAATGAATAATCAAGAC NGG 2 0.4286 Tier II
8 NC_000001.11 1 172360659 - DNM3 NNNGAATGCATGATCCAGAG NGG 2 0.4127 Tier II
9 NC_000014.9 14 37671281 + TTC6 NNNGAATGAGTGATCCAGAG NGG 2 0.4012 Tier II
10 NC_000014.9 14 59546532 + CCDC175 NNNGAATGAAGAATCCAGAG NGG 1 0.4 Tier II
11 NC_000023.11 X 12312085 - FRMPD4 NNNGAATAAATTATCCAGAG NGG 2 0.3333 Tier II
12 NC_000008.11 8 118290192 + SAMD12 NNNTAATGAAAAATCCAGAG NGG 2 0.2727 Tier II
13 NC_000023.11 X 139180033 + FGF13 NNNTAATGAATAATCCAGTG NGG 2 0.1958 Tier II
14 NC_000010.11 10 113111280 - TCF7L2 NNNGAATTAATAATCTAGAG NGG 2 0.1923 Tier II
15 NC_000011.10 11 73492683 - FAM168A NNNTAATGAATAATCCAGAG NAG 2 0.0943 Tier II
16 NC_000002.12 2 102842257 - TMEM182 NNNGAATAAATAATGCAGAG NGG 2 0.05 Tier II
17 NC_000002.12 2 75526162 + EVA1A NNNGAATGAATAATCCAGAA NTG 2 0.0365 Tier II
18 NC_000007.14 7 148241563 + CNTNAP2 NNNGAATGAATAATCCTGAG NAG 2 0.0346 Tier II
19 NC_000014.9 14 52059549 - NID2 NNNGAATGAATGATCCAGAG NGC 2 0.016 Tier II
20 NC_000004.12 4 146716584 + TTC29 NNNTAATGAATAATCCAGAG NTG 2 0.0142 Tier II
21 NC_000011.10 11 17964772 - SERGEF NNNGAATGAATAACCCAGAG NGT 2 0.0046 Tier II
22 NC_000005.10 5 143166512 - ARHGAP26 NNNGAAAGAATAAGCCAGAG NGG 2 0.0 Tier II
23 NC_000005.10 5 168251167 + TENM2 NNNGAATGAATCAGCCAGAG NGG 2 0.0 Tier II
24 NC_000020.11 20 9580453 + LOC105372523 NNNGAATGAATAATCCAGAG NGG 0 1.0 Tier III
25 NC_000023.11 X 96432571 + LOC107985714 NNNGAATGAATAATCCAGAA NGG 1 0.9375 Tier III
26 NC_000010.11 10 83609651 + LOC105378396 NNNGAATGTAAAATCCAGAG NGG 2 0.45 Tier III
27 NC_000005.10 5 171480131 + LOC105377721 NNNGAATGAATGAACCAGAG NGG 2 0.4471 Tier III
28 NC_000002.12 2 176187854 - HAGLR NNNGAATAAAGAATCCAGAG NGG 2 0.4 Tier III
29 NC_000004.12 4 6571579 + LOC105374365 NNNGAATGAATAATCCAGGA NGG 2 0.3516 Tier III
30 NC_000008.11 8 118290192 + LOC105375724 NNNTAATGAAAAATCCAGAG NGG 2 0.2727 Tier III
31 NC_000011.10 11 130731565 - LOC105369575 NNNGAATGAACATTCCAGAG NGG 2 0.2 Tier III
32 NC_000023.11 X 139180033 + LOC124905222 NNNTAATGAATAATCCAGTG NGG 2 0.1958 Tier III
33 NC_000011.10 11 115791424 + LINC02698 NNNGACTGAATAATCCAGGG NGG 2 0.1705 Tier III
34 NC_000011.10 11 115791424 + LOC107987165 NNNGACTGAATAATCCAGGG NGG 2 0.1705 Tier III
35 NC_000001.11 1 20360038 - LINC01141 NNNGAATGAATCCTCCAGAG NGG 2 0.0554 Tier III
36 NC_000008.11 8 144978051 - ZNF252P NNNGGATGAATAATCCAGAG NGA 2 0.05 Tier III
37 NC_000006.12 6 74388463 - LOC101928516 NNNGAATTAATAATTCAGAG NGG 2 0.0417 Tier III
38 NC_000002.12 2 75526162 + EVA1A-AS NNNGAATGAATAATCCAGAA NTG 2 0.0365 Tier III
39 NC_000009.12 9 95852575 - ERCC6L2-AS1 NNNGAATGAATAAGTCAGAG NGG 2 0.0 Tier III
40 NC_000009.12 9 95852575 - LOC124902221 NNNGAATGAATAAGTCAGAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 136116485 + Pak7 NNNGAATGAGTAGTCCAGAG NGG 2 0.3623 Tier I
2 NC_000081.6 15 88898054 + Ttll8 NNNGAATGATAAATCCAGAG NGG 2 0.6618 Tier II
3 NC_000082.6 16 56848142 - Tmem45a NNNGAATGAATAAACAAGAG NGG 2 0.619 Tier II
4 NC_000073.6 7 45633621 + Rasip1 NNNGAAGAAATAATCCAGAG NGG 2 0.5882 Tier II
5 NC_000082.6 16 89950738 - Tiam1 NNNGAATGAATGAACCAGAG NGG 2 0.4471 Tier II
6 NC_000070.6 4 116321030 - Mast2 NNNGACAGAATAATCCAGAG NGG 2 0.3977 Tier II
7 NC_000080.6 14 66890709 + Dpysl2 NNNGAATGAACAATCCACAG NGG 2 0.3175 Tier II
8 NC_000068.7 2 20726645 - Etl4 NNNGATTGAAGAATCCAGAG NGG 2 0.2857 Tier II
9 NC_000067.6 1 38604568 - Aff3 NNNGAAGGAATAATCCAGAC NGG 2 0.2521 Tier II
10 NC_000086.7 X 95920825 + Las1l NNNGAATGTAGAATCCAGAG NGG 2 0.24 Tier II
11 NC_000086.7 X 95920825 + Zc3h12b NNNGAATGTAGAATCCAGAG NGG 2 0.24 Tier II
12 NC_000076.6 10 39497420 + Fyn NNNGAATGAGGAATCCAGAG NGG 2 0.2222 Tier II
13 NC_000086.7 X 143807164 + Capn6 NNNAAATGAATACTCCAGAG NGG 2 0.1895 Tier II
14 NC_000083.6 17 70184032 - Dlgap1 NNNGAATGAATCATCTAGAG NGG 2 0.081 Tier II
15 NC_000076.6 10 79384463 - Vmn2r82 NNNGAATGAATTCTCCAGAG NGG 2 0.0702 Tier II
16 NC_000086.7 X 96339548 + Hsf3 NNNGAATAAATAATCCAGAG NGA 2 0.0694 Tier II
17 NC_000067.6 1 82729802 - Mff NNNGAATGAAAAATGCAGAG NGG 2 0.0375 Tier II
18 NC_000085.6 19 18946064 - Rorb NNNCAATGAATAATTCAGAG NGG 2 0.0353 Tier II
19 NC_000081.6 15 34960456 - Stk3 NNNGAATGACTAATCCAGAG NGA 2 0.0231 Tier II
20 NC_000084.6 18 22983513 + Nol4 NNNGAATGAATAATTTAGAG NGG 2 0.0205 Tier II
21 NC_000082.6 16 59670300 - Epha6 NNNGAATGAAGAATCCAGAG NTG 2 0.0156 Tier II
22 NC_000078.6 12 56898187 + Slc25a21 NNNTAATGAATAATCCAGAG NGC 2 0.0081 Tier II
23 NC_000086.7 X 48864780 - Fsip2l NNNGAATGAAAAAGCCAGAG NGG 2 0.0 Tier II
24 NC_000074.6 8 13640247 + Rasa3 NNNGAATGAATAAGCCAGAG NTG 2 0.0 Tier II
25 NC_000074.6 8 17316066 + Csmd1 NNNGAATGAATAAGCCAGAG NAG 2 0.0 Tier II
26 NC_000078.6 12 5865762 + Gm9548 NNNGAATGAATGATCAAGAG NGG 2 0.7222 Tier III
27 NC_000081.6 15 88898054 + Gm38643 NNNGAATGATAAATCCAGAG NGG 2 0.6618 Tier III
28 NC_000080.6 14 32731617 - Gm28651 NNNGAATAAATAATCCAGAG NAG 2 0.2593 Tier III
29 NC_000086.7 X 69995691 + 1700111N16Rik NNNTAATGAATAATCCAAAG NGG 2 0.2517 Tier III
30 NC_000067.6 1 144108047 - Gm35356 NNNGAATGAATATTCCATAG NGG 2 0.2 Tier III
31 NC_000081.6 15 51917308 + Gm34678 NNNGAATGAATAATCGACAG NGG 2 0.0733 Tier III
32 NC_000084.6 18 68606910 - Gm30821 NNNGAAAGAATAATGCAGAG NGG 2 0.0438 Tier III
33 NC_000070.6 4 123912969 - D130007C19Rik NNNGAATGAATAATAGAGAG NGG 2 0.0342 Tier III
34 NC_000069.6 3 143984344 + Gm33988 NNNGACTGAATAATTCAGAG NGG 2 0.0303 Tier III
35 NC_000079.6 13 54390699 - LOC115488208 NNNGACTGAATAATGCAGAG NGG 2 0.0227 Tier III
36 NC_000082.6 16 59670300 - 4930547E14Rik NNNGAATGAAGAATCCAGAG NTG 2 0.0156 Tier III
37 NC_000081.6 15 24989093 + Gm2824 NNNGAATGAATACTGCAGAG NGG 2 0.0105 Tier III
38 NC_000078.6 12 56898187 + Gm30001 NNNTAATGAATAATCCAGAG NGC 2 0.0081 Tier III
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Other clones with same target sequence:

(none)