Construct: sgRNA BRDN0001149524
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGAATGAATAATCCAGAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PAK5 (57144)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000020.11 | 20 | 9580453 | + | PAK5 | NNNGAATGAATAATCCAGAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000009.12 | 9 | 32570996 | + | NDUFB6 | NNNTAATGAATAATCCAGAC | NGG | 2 | 0.1558 | Tier I |
3 | NC_000004.12 | 4 | 167069934 | - | SPOCK3 | NNNGAATGAAAAATCCAGAA | NGG | 2 | 0.7031 | Tier II |
4 | NC_000005.10 | 5 | 64355288 | + | RNF180 | NNNGAATGAACAATCCAGAA | NGG | 2 | 0.625 | Tier II |
5 | NC_000017.11 | 17 | 66540454 | + | PRKCA | NNNGGATGAATAATCCAAAG | NGG | 2 | 0.4985 | Tier II |
6 | NC_000013.11 | 13 | 94490068 | + | DCT | NNNGAATGAATAAACCAGAT | NGG | 2 | 0.4333 | Tier II |
7 | NC_000012.12 | 12 | 64828619 | - | TBC1D30 | NNNGAATGAATAATCAAGAC | NGG | 2 | 0.4286 | Tier II |
8 | NC_000001.11 | 1 | 172360659 | - | DNM3 | NNNGAATGCATGATCCAGAG | NGG | 2 | 0.4127 | Tier II |
9 | NC_000014.9 | 14 | 37671281 | + | TTC6 | NNNGAATGAGTGATCCAGAG | NGG | 2 | 0.4012 | Tier II |
10 | NC_000014.9 | 14 | 59546532 | + | CCDC175 | NNNGAATGAAGAATCCAGAG | NGG | 1 | 0.4 | Tier II |
11 | NC_000023.11 | X | 12312085 | - | FRMPD4 | NNNGAATAAATTATCCAGAG | NGG | 2 | 0.3333 | Tier II |
12 | NC_000008.11 | 8 | 118290192 | + | SAMD12 | NNNTAATGAAAAATCCAGAG | NGG | 2 | 0.2727 | Tier II |
13 | NC_000023.11 | X | 139180033 | + | FGF13 | NNNTAATGAATAATCCAGTG | NGG | 2 | 0.1958 | Tier II |
14 | NC_000010.11 | 10 | 113111280 | - | TCF7L2 | NNNGAATTAATAATCTAGAG | NGG | 2 | 0.1923 | Tier II |
15 | NC_000011.10 | 11 | 73492683 | - | FAM168A | NNNTAATGAATAATCCAGAG | NAG | 2 | 0.0943 | Tier II |
16 | NC_000002.12 | 2 | 102842257 | - | TMEM182 | NNNGAATAAATAATGCAGAG | NGG | 2 | 0.05 | Tier II |
17 | NC_000002.12 | 2 | 75526162 | + | EVA1A | NNNGAATGAATAATCCAGAA | NTG | 2 | 0.0365 | Tier II |
18 | NC_000007.14 | 7 | 148241563 | + | CNTNAP2 | NNNGAATGAATAATCCTGAG | NAG | 2 | 0.0346 | Tier II |
19 | NC_000014.9 | 14 | 52059549 | - | NID2 | NNNGAATGAATGATCCAGAG | NGC | 2 | 0.016 | Tier II |
20 | NC_000004.12 | 4 | 146716584 | + | TTC29 | NNNTAATGAATAATCCAGAG | NTG | 2 | 0.0142 | Tier II |
21 | NC_000011.10 | 11 | 17964772 | - | SERGEF | NNNGAATGAATAACCCAGAG | NGT | 2 | 0.0046 | Tier II |
22 | NC_000005.10 | 5 | 143166512 | - | ARHGAP26 | NNNGAAAGAATAAGCCAGAG | NGG | 2 | 0.0 | Tier II |
23 | NC_000005.10 | 5 | 168251167 | + | TENM2 | NNNGAATGAATCAGCCAGAG | NGG | 2 | 0.0 | Tier II |
24 | NC_000020.11 | 20 | 9580453 | + | LOC105372523 | NNNGAATGAATAATCCAGAG | NGG | 0 | 1.0 | Tier III |
25 | NC_000023.11 | X | 96432571 | + | LOC107985714 | NNNGAATGAATAATCCAGAA | NGG | 1 | 0.9375 | Tier III |
26 | NC_000010.11 | 10 | 83609651 | + | LOC105378396 | NNNGAATGTAAAATCCAGAG | NGG | 2 | 0.45 | Tier III |
27 | NC_000005.10 | 5 | 171480131 | + | LOC105377721 | NNNGAATGAATGAACCAGAG | NGG | 2 | 0.4471 | Tier III |
28 | NC_000002.12 | 2 | 176187854 | - | HAGLR | NNNGAATAAAGAATCCAGAG | NGG | 2 | 0.4 | Tier III |
29 | NC_000004.12 | 4 | 6571579 | + | LOC105374365 | NNNGAATGAATAATCCAGGA | NGG | 2 | 0.3516 | Tier III |
30 | NC_000008.11 | 8 | 118290192 | + | LOC105375724 | NNNTAATGAAAAATCCAGAG | NGG | 2 | 0.2727 | Tier III |
31 | NC_000011.10 | 11 | 130731565 | - | LOC105369575 | NNNGAATGAACATTCCAGAG | NGG | 2 | 0.2 | Tier III |
32 | NC_000023.11 | X | 139180033 | + | LOC124905222 | NNNTAATGAATAATCCAGTG | NGG | 2 | 0.1958 | Tier III |
33 | NC_000011.10 | 11 | 115791424 | + | LINC02698 | NNNGACTGAATAATCCAGGG | NGG | 2 | 0.1705 | Tier III |
34 | NC_000011.10 | 11 | 115791424 | + | LOC107987165 | NNNGACTGAATAATCCAGGG | NGG | 2 | 0.1705 | Tier III |
35 | NC_000001.11 | 1 | 20360038 | - | LINC01141 | NNNGAATGAATCCTCCAGAG | NGG | 2 | 0.0554 | Tier III |
36 | NC_000008.11 | 8 | 144978051 | - | ZNF252P | NNNGGATGAATAATCCAGAG | NGA | 2 | 0.05 | Tier III |
37 | NC_000006.12 | 6 | 74388463 | - | LOC101928516 | NNNGAATTAATAATTCAGAG | NGG | 2 | 0.0417 | Tier III |
38 | NC_000002.12 | 2 | 75526162 | + | EVA1A-AS | NNNGAATGAATAATCCAGAA | NTG | 2 | 0.0365 | Tier III |
39 | NC_000009.12 | 9 | 95852575 | - | ERCC6L2-AS1 | NNNGAATGAATAAGTCAGAG | NGG | 2 | 0.0 | Tier III |
40 | NC_000009.12 | 9 | 95852575 | - | LOC124902221 | NNNGAATGAATAAGTCAGAG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 136116485 | + | Pak7 | NNNGAATGAGTAGTCCAGAG | NGG | 2 | 0.3623 | Tier I |
2 | NC_000081.6 | 15 | 88898054 | + | Ttll8 | NNNGAATGATAAATCCAGAG | NGG | 2 | 0.6618 | Tier II |
3 | NC_000082.6 | 16 | 56848142 | - | Tmem45a | NNNGAATGAATAAACAAGAG | NGG | 2 | 0.619 | Tier II |
4 | NC_000073.6 | 7 | 45633621 | + | Rasip1 | NNNGAAGAAATAATCCAGAG | NGG | 2 | 0.5882 | Tier II |
5 | NC_000082.6 | 16 | 89950738 | - | Tiam1 | NNNGAATGAATGAACCAGAG | NGG | 2 | 0.4471 | Tier II |
6 | NC_000070.6 | 4 | 116321030 | - | Mast2 | NNNGACAGAATAATCCAGAG | NGG | 2 | 0.3977 | Tier II |
7 | NC_000080.6 | 14 | 66890709 | + | Dpysl2 | NNNGAATGAACAATCCACAG | NGG | 2 | 0.3175 | Tier II |
8 | NC_000068.7 | 2 | 20726645 | - | Etl4 | NNNGATTGAAGAATCCAGAG | NGG | 2 | 0.2857 | Tier II |
9 | NC_000067.6 | 1 | 38604568 | - | Aff3 | NNNGAAGGAATAATCCAGAC | NGG | 2 | 0.2521 | Tier II |
10 | NC_000086.7 | X | 95920825 | + | Las1l | NNNGAATGTAGAATCCAGAG | NGG | 2 | 0.24 | Tier II |
11 | NC_000086.7 | X | 95920825 | + | Zc3h12b | NNNGAATGTAGAATCCAGAG | NGG | 2 | 0.24 | Tier II |
12 | NC_000076.6 | 10 | 39497420 | + | Fyn | NNNGAATGAGGAATCCAGAG | NGG | 2 | 0.2222 | Tier II |
13 | NC_000086.7 | X | 143807164 | + | Capn6 | NNNAAATGAATACTCCAGAG | NGG | 2 | 0.1895 | Tier II |
14 | NC_000083.6 | 17 | 70184032 | - | Dlgap1 | NNNGAATGAATCATCTAGAG | NGG | 2 | 0.081 | Tier II |
15 | NC_000076.6 | 10 | 79384463 | - | Vmn2r82 | NNNGAATGAATTCTCCAGAG | NGG | 2 | 0.0702 | Tier II |
16 | NC_000086.7 | X | 96339548 | + | Hsf3 | NNNGAATAAATAATCCAGAG | NGA | 2 | 0.0694 | Tier II |
17 | NC_000067.6 | 1 | 82729802 | - | Mff | NNNGAATGAAAAATGCAGAG | NGG | 2 | 0.0375 | Tier II |
18 | NC_000085.6 | 19 | 18946064 | - | Rorb | NNNCAATGAATAATTCAGAG | NGG | 2 | 0.0353 | Tier II |
19 | NC_000081.6 | 15 | 34960456 | - | Stk3 | NNNGAATGACTAATCCAGAG | NGA | 2 | 0.0231 | Tier II |
20 | NC_000084.6 | 18 | 22983513 | + | Nol4 | NNNGAATGAATAATTTAGAG | NGG | 2 | 0.0205 | Tier II |
21 | NC_000082.6 | 16 | 59670300 | - | Epha6 | NNNGAATGAAGAATCCAGAG | NTG | 2 | 0.0156 | Tier II |
22 | NC_000078.6 | 12 | 56898187 | + | Slc25a21 | NNNTAATGAATAATCCAGAG | NGC | 2 | 0.0081 | Tier II |
23 | NC_000086.7 | X | 48864780 | - | Fsip2l | NNNGAATGAAAAAGCCAGAG | NGG | 2 | 0.0 | Tier II |
24 | NC_000074.6 | 8 | 13640247 | + | Rasa3 | NNNGAATGAATAAGCCAGAG | NTG | 2 | 0.0 | Tier II |
25 | NC_000074.6 | 8 | 17316066 | + | Csmd1 | NNNGAATGAATAAGCCAGAG | NAG | 2 | 0.0 | Tier II |
26 | NC_000078.6 | 12 | 5865762 | + | Gm9548 | NNNGAATGAATGATCAAGAG | NGG | 2 | 0.7222 | Tier III |
27 | NC_000081.6 | 15 | 88898054 | + | Gm38643 | NNNGAATGATAAATCCAGAG | NGG | 2 | 0.6618 | Tier III |
28 | NC_000080.6 | 14 | 32731617 | - | Gm28651 | NNNGAATAAATAATCCAGAG | NAG | 2 | 0.2593 | Tier III |
29 | NC_000086.7 | X | 69995691 | + | 1700111N16Rik | NNNTAATGAATAATCCAAAG | NGG | 2 | 0.2517 | Tier III |
30 | NC_000067.6 | 1 | 144108047 | - | Gm35356 | NNNGAATGAATATTCCATAG | NGG | 2 | 0.2 | Tier III |
31 | NC_000081.6 | 15 | 51917308 | + | Gm34678 | NNNGAATGAATAATCGACAG | NGG | 2 | 0.0733 | Tier III |
32 | NC_000084.6 | 18 | 68606910 | - | Gm30821 | NNNGAAAGAATAATGCAGAG | NGG | 2 | 0.0438 | Tier III |
33 | NC_000070.6 | 4 | 123912969 | - | D130007C19Rik | NNNGAATGAATAATAGAGAG | NGG | 2 | 0.0342 | Tier III |
34 | NC_000069.6 | 3 | 143984344 | + | Gm33988 | NNNGACTGAATAATTCAGAG | NGG | 2 | 0.0303 | Tier III |
35 | NC_000079.6 | 13 | 54390699 | - | LOC115488208 | NNNGACTGAATAATGCAGAG | NGG | 2 | 0.0227 | Tier III |
36 | NC_000082.6 | 16 | 59670300 | - | 4930547E14Rik | NNNGAATGAAGAATCCAGAG | NTG | 2 | 0.0156 | Tier III |
37 | NC_000081.6 | 15 | 24989093 | + | Gm2824 | NNNGAATGAATACTGCAGAG | NGG | 2 | 0.0105 | Tier III |
38 | NC_000078.6 | 12 | 56898187 | + | Gm30001 | NNNTAATGAATAATCCAGAG | NGC | 2 | 0.0081 | Tier III |