Construct: sgRNA BRDN0001162196
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTCGAAGAGTCATGCATAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK7 (140609)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77740
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
198278065 |
+ |
NEK7 |
NNNCGAAGAGTCATGCATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
51230288 |
+ |
PRKG1 |
NNNCAAAGAGACATGCATAG |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000023.11 |
X |
105028606 |
+ |
IL1RAPL2 |
NNNTGAGGAGTCATGCATAG |
NGG |
2 |
0.5647 |
Tier II |
| 4 |
NC_000023.11 |
X |
100907671 |
- |
XKRX |
NNNCGAAGAGTTATGCATAG |
NGG |
1 |
0.5385 |
Tier II |
| 5 |
NC_000008.11 |
8 |
22222275 |
- |
PHYHIP |
NNNGGAAGAGTCCTGCATAG |
NGG |
2 |
0.1053 |
Tier II |
| 6 |
NC_000001.11 |
1 |
213396067 |
+ |
RPS6KC1 |
NNNCGAAGAGTCAGGCTTAG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
138516533 |
- |
Nek7 |
NNNCGAAGAGTCATGCACAG |
NGG |
1 |
0.4286 |
Tier I |
| 2 |
NC_000075.6 |
9 |
29418458 |
- |
Ntm |
NNNGGAAGAGACATGCATAG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000073.6 |
7 |
111692557 |
+ |
Galnt18 |
NNNCGAAGTCTCATGCATAG |
NGG |
2 |
0.24 |
Tier II |
| 4 |
NC_000077.6 |
11 |
84171400 |
+ |
Acaca |
NNNTGAAGAGTCATGGATAG |
NGG |
2 |
0.1231 |
Tier II |
| 5 |
NC_000074.6 |
8 |
92991746 |
+ |
Slc6a2 |
NNNCAAAGAGTCATGCTTAG |
NGG |
2 |
0.1156 |
Tier II |
| 6 |
NC_000082.6 |
16 |
42783011 |
+ |
4932412D23Rik |
NNNCAAAGATTCATGCATAG |
NGG |
2 |
0.7042 |
Tier III |
| 7 |
NC_000069.6 |
3 |
138085363 |
- |
Gm52617 |
NNNCAAAGAGTCATGCCTAG |
NGG |
2 |
0.1529 |
Tier III |
| 8 |
NC_000073.6 |
7 |
38044859 |
- |
Gm30684 |
NNNCGAAGAGTCATCCTTAG |
NGG |
2 |
0.0364 |
Tier III |
Other clones with same target sequence:
(none)
