Construct: sgRNA BRDN0001162216

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTAAAAACCTTACCCTATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK7 (1022)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76077

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 69258150 - CDK7 NNNAAAACCTTACCCTATGT NGG 0 1.0 Tier I
2 NC_000004.12 4 70754835 - RUFY3 NNNAAAAGCTTACTCTATGT NGG 2 0.4714 Tier II
3 NC_000006.12 6 128468967 - PTPRK NNNAGAAACTTACCCTATGT NGG 2 0.468 Tier II
4 NC_000004.12 4 86521299 + MAPK10 NNNATAATCTTACCCTATGT NGG 2 0.3182 Tier II
5 NC_000005.10 5 119214783 - DMXL1 NNNAAAACCTTTCTCTATGT NGG 2 0.2444 Tier II
6 NC_000010.11 10 67589773 + CTNNA3 NNNAGAACCTTACCCTCTGT NGG 2 0.1271 Tier II
7 NC_000005.10 5 177525415 - FAM193B NNNAAAACCCTACCCTCTGT NGG 2 0.0941 Tier II
8 NC_000012.12 12 43501800 - ADAMTS20 NNNAAAACCTTACCCTATTT NTG 2 0.026 Tier II
9 NC_000023.11 X 75209695 - UPRT NNNAAAACCTTACCCTATCT NTG 2 0.0175 Tier II
10 NC_000014.9 14 90748765 + TTC7B NNNAAAACCTTACCCTATGT NGT 1 0.0161 Tier II
11 NC_000010.11 10 43388411 - HNRNPF NNNAAAATCTTACCCTATGT NGT 2 0.0141 Tier II
12 NC_000011.10 11 31057627 - DCDC1 NNNAAAACCTTACCCTCTGT NTG 2 0.0069 Tier II
13 NC_000002.12 2 115453090 + DPP10 NNNAAAACCTTACCTTATGT NTG 2 0.0026 Tier II
14 NC_000005.10 5 125357473 + LOC101927421 NNNACAAGCTTACCCTATGT NGG 2 0.3214 Tier III
15 NC_000011.10 11 131216737 + LOC107984412 NNNAAAACCTTAGCATATGT NGG 2 0.0303 Tier III
16 NC_000024.10 Y 18591242 + OFD1P5Y NNNAAAACCTTAACCTATGT NGA 2 0.0267 Tier III
17 NC_000024.10 Y 18729162 - OFD1P6Y NNNAAAACCTTAACCTATGT NGA 2 0.0267 Tier III
18 NC_000007.14 7 65507982 - LOC101929322 NNNAAAACCTTACCTTTTGT NGG 2 0.0089 Tier III
19 NC_000007.14 7 65553777 - LOC101929322 NNNAAAACCTTACCTTTTGT NGG 2 0.0089 Tier III
20 NC_000007.14 7 65648849 + LINC03006 NNNAAAACCTTACCTTTTGT NGG 2 0.0089 Tier III
21 NC_000007.14 7 65648849 + INTS4P2 NNNAAAACCTTACCTTTTGT NGG 2 0.0089 Tier III
22 NC_000010.11 10 36433653 - MTND5P17 NNNAAAACCTTACCCTATGT NTA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 24890252 + Ehmt1 NNNAAGACCTAACCCTATGT NGG 2 0.5357 Tier II
2 NC_000073.6 7 143364731 - Kcnq1 NNNAAAGCCTTATCCTATGT NGG 2 0.4941 Tier II
3 NC_000076.6 10 85646438 - Btbd11 NNNAAATCCTCACCCTATGT NGG 2 0.2917 Tier II
4 NC_000067.6 1 106025027 + Zcchc2 NNNCAAACCTTATCCTATGT NGG 2 0.2471 Tier II
5 NC_000079.6 13 49563692 - Cenpp NNNAAAACCTTACCCTTTGT NGG 1 0.1333 Tier II
6 NC_000079.6 13 49563692 - Aspn NNNAAAACCTTACCCTTTGT NGG 1 0.1333 Tier II
7 NC_000075.6 9 89734454 + Ankrd34c NNNAAAACATTACCCTATGC NGG 2 0.0779 Tier II
8 NC_000075.6 9 116850338 - Rbms3 NNNAAAACATTACCTTATGT NGG 2 0.0571 Tier II
9 NC_000073.6 7 133117035 - Ctbp2 NNNAATACCTTACCCTATGT NTG 2 0.0278 Tier II
10 NC_000076.6 10 9812804 + Stxbp5 NNNAAAACCTTACCCTCTGC NGG 2 0.016 Tier II
11 NC_000073.6 7 65588956 - BC046251 NNNAAAACCTTACCCAATGA NGG 2 0.5114 Tier III
12 NC_000076.6 10 34241101 - Gm26564 NNNAAAACATTACCATATGT NGG 2 0.1905 Tier III
13 NC_000067.6 1 63920116 + Gm13754 NNNAAAACCTTACCCTATTT NGC 2 0.0148 Tier III
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Other clones with same target sequence:

(none)