Construct: sgRNA BRDN0001162231
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTCCCTCAATGGTACACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERN1 (2081)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76409
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
64065228 |
- |
ERN1 |
NNNCCCTCAATGGTACACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
3880887 |
- |
CSMD1 |
NNNCTTTCAATGGTACACGA |
NGG |
2 |
0.5909 |
Tier II |
| 3 |
NC_000011.10 |
11 |
75090227 |
- |
OR2AT4 |
NNNCCCTCAATGGAACATGA |
NGG |
2 |
0.398 |
Tier II |
| 4 |
NC_000001.11 |
1 |
84224665 |
+ |
PRKACB |
NNNCCCTCAATGGTGCACGT |
NGG |
2 |
0.39 |
Tier II |
| 5 |
NC_000013.11 |
13 |
94014127 |
+ |
GPC6 |
NNNCTCTCAATGGTACAAGA |
NGG |
2 |
0.3427 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
106420038 |
- |
Ern1 |
NNNCCCTCAATGGTGCATGA |
NGG |
2 |
0.4179 |
Tier I |
| 2 |
NC_000080.6 |
14 |
34961170 |
+ |
Grid1 |
NNNCCCTCACTGGTACATGA |
NGG |
2 |
0.2143 |
Tier II |
| 3 |
NC_000084.6 |
18 |
84208479 |
+ |
Zfp407 |
NNNCCCACAATGGTACTCGA |
NGG |
2 |
0.1167 |
Tier II |
Other clones with same target sequence:
(none)
