Construct: sgRNA BRDN0001162271
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCAGGGTTAGTGAAGCTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAOK2 (9344)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77252
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
29979245 |
+ |
TAOK2 |
NNNAGGGTTAGTGAAGCTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
138924164 |
+ |
CTNNA1 |
NNNAGAGTCAGTGAAGCTAG |
NGG |
2 |
0.9231 |
Tier II |
| 3 |
NC_000005.10 |
5 |
148632475 |
+ |
HTR4 |
NNNAGGGTCAGTGAAGCAAG |
NGG |
2 |
0.6154 |
Tier II |
| 4 |
NC_000011.10 |
11 |
66262433 |
- |
KLC2 |
NNNAGGGTCAGGGAAGCTAG |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000006.12 |
6 |
53056033 |
+ |
CILK1 |
NNNAGGGATAGTGAAGATAG |
NGG |
2 |
0.3733 |
Tier II |
| 6 |
NC_000005.10 |
5 |
161545773 |
- |
GABRB2 |
NNNAGGGTTAGAGAAGGTAG |
NGG |
2 |
0.0471 |
Tier II |
| 7 |
NC_000001.11 |
1 |
174644201 |
+ |
RABGAP1L |
NNNAGGGTTTGTGAAGCTAG |
NGT |
2 |
0.0142 |
Tier II |
| 8 |
NC_000023.11 |
X |
124966307 |
- |
TENM1 |
NNNAGGGTTAGTGCAGCTAG |
NGT |
2 |
0.0035 |
Tier II |
| 9 |
NC_000011.10 |
11 |
66262433 |
- |
KLC2-AS1 |
NNNAGGGTCAGGGAAGCTAG |
NGG |
2 |
0.4615 |
Tier III |
| 10 |
NC_000004.12 |
4 |
187209546 |
- |
LINC02374 |
NNNAGTGTTAGTGAAGCTAG |
NTG |
2 |
0.026 |
Tier III |
| 11 |
NC_000010.11 |
10 |
122786290 |
- |
DMBT1L1 |
NNNAGGGTTAATGAAGCTAG |
NGT |
2 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
182883557 |
+ |
Susd4 |
NNNAGGTTTAGTGAAGCTAA |
NGG |
2 |
0.5357 |
Tier II |
| 2 |
NC_000077.6 |
11 |
117200694 |
- |
Septin9 |
NNNAGGGTTTGTGAAGTTAG |
NGG |
2 |
0.4118 |
Tier II |
| 3 |
NC_000072.6 |
6 |
36455955 |
- |
Chrm2 |
NNNAGGGTTAGTGAAACTGG |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000082.6 |
16 |
31120604 |
+ |
Acap2 |
NNNAGGGTTAGAGAAGTTAG |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000081.6 |
15 |
89178021 |
+ |
Plxnb2 |
NNNAGGGTTGGTGAAGCTAG |
NAG |
2 |
0.144 |
Tier II |
| 6 |
NC_000079.6 |
13 |
8712444 |
- |
Adarb2 |
NNNAGGGTTAGGGAAGCTAG |
NAG |
2 |
0.1296 |
Tier II |
| 7 |
NC_000082.6 |
16 |
44360280 |
+ |
Spice1 |
NNNAGAGTTAGTGAAGCTAG |
NTG |
2 |
0.039 |
Tier II |
| 8 |
NC_000071.6 |
5 |
139182618 |
- |
Dnaaf5 |
NNNAGGGCTAGTGAAGCTAG |
NGT |
2 |
0.0161 |
Tier II |
| 9 |
NC_000083.6 |
17 |
24122342 |
- |
Pdpk1 |
NNNAGGGTTAGTGAAGCTAC |
NGT |
2 |
0.0069 |
Tier II |
| 10 |
NC_000071.6 |
5 |
9089557 |
- |
Gm31449 |
NNNTGGGTTAGTGTAGCTAG |
NGG |
2 |
0.3394 |
Tier III |
| 11 |
NC_000070.6 |
4 |
24027248 |
- |
Gm27243 |
NNNATGTTTAGTGAAGCTAG |
NGG |
2 |
0.1714 |
Tier III |
Other clones with same target sequence:
(none)
