Construct: sgRNA BRDN0001162301
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGTCTCCGCATCGTCTTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80197
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
72364066 |
- |
ZFAND5 |
NNNTCTCGGCGTCGTCTTTT |
NGG |
2 |
0.4179 |
Tier II |
| 2 |
NC_000002.12 |
2 |
84504019 |
+ |
DNAH6 |
NNNTCTCCACATCCTCTTTT |
NGG |
2 |
0.2755 |
Tier II |
| 3 |
NC_000003.12 |
3 |
119801922 |
+ |
NR1I2 |
NNNTCTCCGCATGGTCTCTT |
NGG |
2 |
0.0584 |
Tier II |
| 4 |
NC_000019.10 |
19 |
16308347 |
- |
KLF2-DT |
NNNTCTCAGCATCGTGTTTT |
NGG |
2 |
0.1 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
9574584 |
- |
Zfp704 |
NNNTCTCCGCAGCGCCTTTT |
NGG |
2 |
0.1364 |
Tier II |
| 2 |
NC_000073.6 |
7 |
5825909 |
+ |
Vmn1r63 |
NNNTCTCAGCATGGTCTTTT |
NGG |
2 |
0.0886 |
Tier II |
| 3 |
NT_166307.1 |
7 |
172441 |
+ |
Vmn1r187 |
NNNTCTCAGCATGGTCTTTT |
NGG |
2 |
0.0886 |
Tier II |
| 4 |
NC_000072.6 |
6 |
141009899 |
+ |
Gm30524 |
NNNTCTCCTCATTGTCTTTT |
NGG |
2 |
0.3733 |
Tier III |
| 5 |
NC_000073.6 |
7 |
5825909 |
+ |
Vmn1r-ps44 |
NNNTCTCAGCATGGTCTTTT |
NGG |
2 |
0.0886 |
Tier III |
| 6 |
NT_166307.1 |
7 |
172441 |
+ |
Vmn1r-ps91 |
NNNTCTCAGCATGGTCTTTT |
NGG |
2 |
0.0886 |
Tier III |
Other clones with same target sequence:
(none)
