Construct: sgRNA BRDN0001162319
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAAGCACTGCACGATGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K7 (5609)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77821
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
7910539 |
- |
MAP2K7 |
NNNAGCACTGCACGATGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
131280395 |
- |
LOC105375760 |
NNNAGCACAGCACAATGTAG |
NGG |
2 |
0.6964 |
Tier III |
| 3 |
NC_000008.11 |
8 |
93557096 |
+ |
CIBAR1-DT |
NNNAGCAATGCAGGATGTAG |
NGG |
2 |
0.0886 |
Tier III |
| 4 |
NC_000016.10 |
16 |
80429524 |
+ |
DYNLRB2-AS1 |
NNNAGCACTGCAAGATGTAG |
NGT |
2 |
0.0062 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
4244037 |
- |
Map2k7 |
NNNAGCACTGAACGATGTAA |
NGG |
2 |
0.7031 |
Tier I |
| 2 |
NC_000077.6 |
11 |
120702516 |
- |
Aspscr1 |
NNNAGCCCTGCAGGATGTAG |
NGG |
2 |
0.0597 |
Tier I |
| 3 |
NC_000073.6 |
7 |
46461426 |
- |
Sergef |
NNNAGCATTGCACCATGTAG |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000070.6 |
4 |
132199122 |
- |
Ythdf2 |
NNNAGCACTGCACAATGGAG |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000074.6 |
8 |
61461778 |
- |
Sh3rf1 |
NNNAGCACTGCTCAATGTAG |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000080.6 |
14 |
32574130 |
- |
Ercc6 |
NNNAGCAATGCACGATGTGG |
NGG |
2 |
0.2438 |
Tier II |
| 7 |
NC_000068.7 |
2 |
119528541 |
+ |
Exd1 |
NNNAGCACTGGATGATGTAG |
NGG |
2 |
0.175 |
Tier II |
| 8 |
NC_000080.6 |
14 |
121280571 |
+ |
Farp1 |
NNNGGCACTGCACTATGTAG |
NGG |
2 |
0.1667 |
Tier II |
| 9 |
NC_000080.6 |
14 |
10264949 |
+ |
Fhit |
NNNAACACTGCAGGATGTAG |
NGG |
2 |
0.1182 |
Tier II |
| 10 |
NC_000072.6 |
6 |
90665221 |
+ |
Iqsec1 |
NNNAGCACTGCACCATGTAG |
NCG |
2 |
0.0459 |
Tier II |
Other clones with same target sequence:
(none)
