Construct: sgRNA BRDN0001162324
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAGAGTACTCTTCCACCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERBB4 (2066)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76198
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
211704123 |
+ |
ERBB4 |
NNNGAGTACTCTTCCACCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
49989973 |
- |
ZPBP |
NNNGAGTCCTCTTCCAACAA |
NGG |
2 |
0.2 |
Tier II |
| 3 |
NC_000023.11 |
X |
41204562 |
- |
USP9X |
NNNTAGTACTCTTCCACAAA |
NGG |
2 |
0.1958 |
Tier II |
| 4 |
NC_000017.11 |
17 |
1079004 |
+ |
ABR |
NNNGAGTGCTCTTCCAGCAA |
NGG |
2 |
0.0431 |
Tier II |
| 5 |
NC_000020.11 |
20 |
19759365 |
+ |
RIN2 |
NNNGAGTACTCTTCGACCAA |
NAG |
2 |
0.013 |
Tier II |
| 6 |
NC_000016.10 |
16 |
4439900 |
- |
DNAJA3 |
NNNGAGTACGCTTCCTCCAA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000006.12 |
6 |
100897870 |
- |
LOC107984041 |
NNNGAGTACTCCTCCAACAA |
NGG |
2 |
0.4421 |
Tier III |
| 8 |
NC_000001.11 |
1 |
153793303 |
- |
LOC124904426 |
NNNGACTACCCTTCCACCAA |
NGG |
2 |
0.3636 |
Tier III |
| 9 |
NC_000003.12 |
3 |
51775973 |
- |
LOC105377087 |
NNNCAGTTCTCTTCCACCAA |
NGG |
2 |
0.2269 |
Tier III |
| 10 |
NC_000001.11 |
1 |
238482585 |
+ |
LINC01139 |
NNNGAGTCCTCTTCCAACAA |
NGG |
2 |
0.2 |
Tier III |
| 11 |
NC_000003.12 |
3 |
81990205 |
- |
LINC02008 |
NNNGAGTCCTCTTCCAACAA |
NGG |
2 |
0.2 |
Tier III |
| 12 |
NC_000012.12 |
12 |
9638072 |
+ |
LOC374443 |
NNNGAGTCCTCTTCCAACAA |
NGG |
2 |
0.2 |
Tier III |
| 13 |
NC_000012.12 |
12 |
70471883 |
- |
LOC105369828 |
NNNGAGTCCTCTTCCAACAA |
NGG |
2 |
0.2 |
Tier III |
| 14 |
NC_000023.11 |
X |
87725157 |
+ |
LOC124905233 |
NNNGAGTTCTCTTCCAACAA |
NGG |
2 |
0.2 |
Tier III |
| 15 |
NC_000005.10 |
5 |
68818881 |
+ |
LOC105379013 |
NNNCAGTACTCTTCCACCAA |
NAG |
2 |
0.1373 |
Tier III |
| 16 |
NC_000014.9 |
14 |
23939357 |
- |
DHRS4-AS1 |
NNNGAGTACTCTTCCCCCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
93163997 |
- |
Septin11 |
NNNGAGAACTCCTCCACCAA |
NGG |
2 |
0.8289 |
Tier II |
| 2 |
NC_000067.6 |
1 |
154688509 |
+ |
Cacna1e |
NNNGACTACACTTCCACCAA |
NGG |
2 |
0.5844 |
Tier II |
| 3 |
NC_000068.7 |
2 |
15983487 |
+ |
Malrd1 |
NNNAAGTACTCTTCCAACAA |
NGG |
2 |
0.42 |
Tier II |
| 4 |
NC_000078.6 |
12 |
21293715 |
+ |
Cpsf3 |
NNNCAGTACTGTTCCACCAA |
NGG |
2 |
0.1324 |
Tier II |
| 5 |
NC_000085.6 |
19 |
23420876 |
- |
Mamdc2 |
NNNGAGTACTCTTTCACCAA |
NGT |
2 |
0.0118 |
Tier II |
| 6 |
NC_000067.6 |
1 |
8549566 |
- |
Sntg1 |
NNNGAGTACTTTTCCACCAA |
NGT |
2 |
0.005 |
Tier II |
| 7 |
NC_000082.6 |
16 |
40089898 |
- |
Lsamp |
NNNGAGTACTCATCCTCCAA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000082.6 |
16 |
11580677 |
+ |
Snx29 |
NNNGAGTACTCTTCATCCAA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000074.6 |
8 |
42041852 |
- |
2810404M03Rik |
NNNGAAAACTCTTCCACCAA |
NGG |
2 |
0.875 |
Tier III |
| 10 |
NC_000076.6 |
10 |
15802646 |
+ |
C330004P14Rik |
NNNGAGTACACTTCCGCCAA |
NGG |
2 |
0.1648 |
Tier III |
| 11 |
NC_000076.6 |
10 |
118770101 |
+ |
Gm33439 |
NNNGAGAACTCTTCCCCCAA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
