Construct: sgRNA BRDN0001162372
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGTGTGTCCAGATAGCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERBB4 (2066)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76195
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
211561908 |
- |
ERBB4 |
NNNGTGTGTCCAGATAGCTA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
119586424 |
- |
ADPRH |
NNNGTGTGTCCAGTTGGCTA |
NGG |
2 |
0.1026 |
Tier I |
| 3 |
NC_000011.10 |
11 |
11401898 |
+ |
GALNT18 |
NNNGTGTGTACAGATAGCTA |
NGG |
1 |
0.8667 |
Tier II |
| 4 |
NC_000004.12 |
4 |
31122078 |
- |
PCDH7 |
NNNGTGTGTCCAGATAGTTA |
NGG |
1 |
0.6429 |
Tier II |
| 5 |
NC_000016.10 |
16 |
31376309 |
+ |
ITGAX |
NNNGTTTGACCAGATAGCTA |
NGG |
2 |
0.619 |
Tier II |
| 6 |
NC_000010.11 |
10 |
61919803 |
- |
ARID5B |
NNNGTCTGTCCAGATAGTTA |
NGG |
2 |
0.4383 |
Tier II |
| 7 |
NC_000007.14 |
7 |
21463573 |
+ |
SP4 |
NNNGTGTGTCCACATAACTA |
NGG |
2 |
0.393 |
Tier II |
| 8 |
NC_000002.12 |
2 |
188344324 |
+ |
GULP1 |
NNNGAGTGTGCAGATAGCTA |
NGG |
2 |
0.1944 |
Tier II |
| 9 |
NC_000022.11 |
22 |
25843628 |
- |
MYO18B |
NNNGTGTTTCCAGATAGCTA |
NCG |
2 |
0.067 |
Tier II |
| 10 |
NC_000003.12 |
3 |
96956350 |
+ |
EPHA6 |
NNNGTGTGCCCAGATAGCTA |
NGA |
2 |
0.0641 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
84917801 |
+ |
Mast1 |
NNNGTGTGTCCAGTGAGCTA |
NGG |
2 |
0.0267 |
Tier I |
| 2 |
NC_000067.6 |
1 |
68198847 |
- |
Erbb4 |
NNNGTGTGTCCAGATTGCTA |
NGG |
1 |
0.0 |
Tier I |
| 3 |
NC_000073.6 |
7 |
118828991 |
+ |
Vps35l |
NNNGTCTGTTCAGATAGCTA |
NGG |
2 |
0.6417 |
Tier II |
| 4 |
NC_000086.7 |
X |
142354743 |
- |
Acsl4 |
NNNGTGAGTCCAGATAGTTA |
NGG |
2 |
0.5625 |
Tier II |
| 5 |
NC_000071.6 |
5 |
91005530 |
- |
Mthfd2l |
NNNGTGTGTTCAGACAGCTA |
NGG |
2 |
0.2567 |
Tier II |
| 6 |
NC_000076.6 |
10 |
83008102 |
+ |
Chst11 |
NNNGTGAGTCCAGACAGCTA |
NGG |
2 |
0.2386 |
Tier II |
| 7 |
NC_000078.6 |
12 |
76618765 |
- |
Sptb |
NNNGAGTGTGCAGATAGCTA |
NGG |
2 |
0.1944 |
Tier II |
| 8 |
NC_000071.6 |
5 |
147679930 |
- |
Flt1 |
NNNGTGTGGCCAGCTAGCTA |
NGG |
2 |
0.1327 |
Tier II |
| 9 |
NC_000075.6 |
9 |
21679687 |
+ |
Smarca4 |
NNNGTGTGTCCAGGTAGCCA |
NGG |
2 |
0.1287 |
Tier II |
| 10 |
NC_000080.6 |
14 |
33174848 |
+ |
Wdfy4 |
NNNGTGTGTCCAGGCAGCTA |
NGG |
2 |
0.1273 |
Tier II |
| 11 |
NC_000086.7 |
X |
162969418 |
+ |
Ctps2 |
NNNGAGTGTCCAGCTAGCTA |
NGG |
2 |
0.1071 |
Tier II |
| 12 |
NC_000072.6 |
6 |
115562882 |
+ |
Tsen2 |
NNNGTGTGTCCAGACAGCTA |
NAG |
2 |
0.0707 |
Tier II |
| 13 |
NC_000070.6 |
4 |
129059312 |
+ |
Rnf19b |
NNNGTGTGTCCATATGGCTA |
NGG |
2 |
0.0577 |
Tier II |
| 14 |
NC_000080.6 |
14 |
42418268 |
- |
Gm2959 |
NNNGTGAGTCCAGAGAGCTA |
NGG |
2 |
0.0438 |
Tier II |
| 15 |
NC_000072.6 |
6 |
137776709 |
+ |
Dera |
NNNGTGTGTCCACATAGCTA |
NGA |
2 |
0.0292 |
Tier II |
| 16 |
NC_000079.6 |
13 |
13370006 |
+ |
Gpr137b |
NNNGTGTGTCCAGAGAGATA |
NGG |
2 |
0.0269 |
Tier II |
| 17 |
NC_000079.6 |
13 |
12626311 |
+ |
Gpr137b-ps |
NNNGTGTGTCCAGAGAGATA |
NGG |
2 |
0.0269 |
Tier III |
| 18 |
NC_000074.6 |
8 |
84917801 |
+ |
Gm38426 |
NNNGTGTGTCCAGTGAGCTA |
NGG |
2 |
0.0267 |
Tier III |
| 19 |
NC_000080.6 |
14 |
101262608 |
- |
Gm34361 |
NNNGTGTGTACAGATAGCTA |
NGC |
2 |
0.0193 |
Tier III |
| 20 |
NC_000079.6 |
13 |
39777822 |
- |
Gm40923 |
NNNGTGTGTCCAGATAGCTC |
NGT |
2 |
0.0037 |
Tier III |
Other clones with same target sequence:
(none)
