Construct: sgRNA BRDN0001162420
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGTGGGAGCAACGCCTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPG (55561)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77525
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64835785 |
- |
CDC42BPG |
NNNGTGGGAGCAACGCCTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
5594147 |
- |
SAFB2 |
NNNGCGGGAGCAACGCCTCG |
NGG |
2 |
0.2759 |
Tier I |
| 3 |
NC_000020.11 |
20 |
63649511 |
- |
STMN3 |
NNNGAGGGAGCAATGCCTTG |
NGG |
2 |
0.3667 |
Tier II |
| 4 |
NC_000011.10 |
11 |
66859161 |
- |
PC |
NNNGTGGAAGCAAAGCCTTG |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000011.10 |
11 |
66859161 |
- |
LRFN4 |
NNNGTGGAAGCAAAGCCTTG |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000002.12 |
2 |
7024353 |
- |
RNF144A |
NNNGTGGGAGAAACGCCGTG |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000019.10 |
19 |
3468221 |
- |
NFIC |
NNNGTGTGAGCAAAGCCTTG |
NGG |
2 |
0.2 |
Tier II |
| 8 |
NC_000019.10 |
19 |
44865721 |
+ |
NECTIN2 |
NNNGTGGGAGCATCCCCTTG |
NGG |
2 |
0.0818 |
Tier II |
| 9 |
NC_000022.11 |
22 |
35878677 |
- |
RBFOX2 |
NNNGTGGGAGGAACTCCTTG |
NGG |
2 |
0.0357 |
Tier II |
| 10 |
NC_000022.11 |
22 |
29739764 |
- |
ZMAT5 |
NNNGTGGAAGCAAGGCCTTG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000011.10 |
11 |
45179959 |
- |
PRDM11 |
NNNGTGGGAGCAAGGCCCTG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000020.11 |
20 |
62850932 |
- |
TCFL5 |
NNNGTGGGAGCAGGGCCTTG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000007.14 |
7 |
76502902 |
+ |
DTX2 |
NNNGTGGGAGCCAGGCCTTG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000010.11 |
10 |
79418991 |
+ |
ZCCHC24 |
NNNGTGTGAGCAAGGCCTTG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000012.12 |
12 |
49725137 |
+ |
LOC124902929 |
NNNGTGGGAGCTACGCTTTG |
NGG |
2 |
0.1556 |
Tier III |
| 16 |
NC_000013.11 |
13 |
63632957 |
+ |
LOC105370236 |
NNNTTGGGAGCAAAGCCTTG |
NGG |
2 |
0.1273 |
Tier III |
| 17 |
NC_000007.14 |
7 |
77002767 |
+ |
DTX2P1-UPK3BP1-PMS2P11 |
NNNGTGGGAGCCAGGCCTTG |
NGG |
2 |
0.0 |
Tier III |
| 18 |
NC_000007.14 |
7 |
77002767 |
+ |
DTX2P1 |
NNNGTGGGAGCCAGGCCTTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
125420830 |
+ |
Sipa1l2 |
NNNGTGGAAGCAAAGCCTTG |
NGG |
2 |
0.35 |
Tier II |
| 2 |
NC_000071.6 |
5 |
98167199 |
- |
Prdm8 |
NNNGTGGGAGCAGCGCCTAG |
NGG |
2 |
0.1863 |
Tier II |
| 3 |
NC_000070.6 |
4 |
106752764 |
+ |
Acot11 |
NNNGTGGGAGCAAAGCATTG |
NGG |
2 |
0.1633 |
Tier II |
| 4 |
NC_000077.6 |
11 |
24119502 |
+ |
Bcl11a |
NNNGTGGGAGCAGCTCCTTG |
NGG |
2 |
0.0932 |
Tier II |
| 5 |
NC_000071.6 |
5 |
113903118 |
+ |
Coro1c |
NNNGTGGGTGCAACGCCTTG |
NGC |
2 |
0.0133 |
Tier II |
| 6 |
NC_000083.6 |
17 |
56718980 |
- |
Ndufa11 |
NNNGTGGGAGCAAAGCCTTG |
NGT |
2 |
0.0056 |
Tier II |
| 7 |
NC_000070.6 |
4 |
18032806 |
- |
Mmp16 |
NNNGTGGGAGCAAGGCTTTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000072.6 |
6 |
31666241 |
+ |
Gm13847 |
NNNGTGGGAGCACCACCTTG |
NGG |
2 |
0.1981 |
Tier III |
| 9 |
NC_000072.6 |
6 |
31666241 |
+ |
Gm13846 |
NNNGTGGGAGCACCACCTTG |
NGG |
2 |
0.1981 |
Tier III |
Other clones with same target sequence:
(none)
