Construct: sgRNA BRDN0001162456

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GACCATGGAGCTACATGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STKLD1 (169436)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75813

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 133400470 + STKLD1 NNNCATGGAGCTACATGACA NGG 0 1.0 Tier I
2 NC_000006.12 6 159109435 + LOC124901449 NNNCATGGTGCTACATGAAA NGG 2 0.2571 Tier I
3 NC_000020.11 20 32215262 - POFUT1 NNNCATGGAGCTACAGGAAA NGG 2 0.1484 Tier I
4 NC_000023.11 X 34944378 + FAM47B NNNCATGGAGCTAGACGACA NGG 2 0.0 Tier I
5 NC_000023.11 X 34130295 - FAM47A NNNCATGGAGCTAGATGAAA NGG 2 0.0 Tier I
6 NC_000006.12 6 4788970 - CDYL NNNCATGTAGCTACAAGACA NGG 2 0.5682 Tier II
7 NC_000016.10 16 14104347 + MRTFB NNNCAGGGAGCAACATGACA NGG 2 0.4571 Tier II
8 NC_000008.11 8 142846117 + GML NNNCGTGTAGCTACATGACA NGG 2 0.45 Tier II
9 NC_000017.11 17 79520113 - RBFOX3 NNNCATGGAGCTGCATGACT NGG 2 0.3913 Tier II
10 NC_000006.12 6 159109435 + LOC112267968 NNNCATGGTGCTACATGAAA NGG 2 0.2571 Tier II
11 NC_000006.12 6 159109480 + LOC112267968 NNNCATGGTGCTACATGAAA NGG 2 0.2571 Tier II
12 NC_000006.12 6 159109480 + LOC124901449 NNNCATGGTGCTACATGAAA NGG 2 0.2571 Tier II
13 NC_000022.11 22 20797744 + PI4KA NNNCATGCAGCTACATGGCA NGG 2 0.2462 Tier II
14 NC_000018.10 18 53394159 + DCC NNNCATGGAGCCACATGACC NGG 2 0.2153 Tier II
15 NC_000017.11 17 14104043 - COX10 NNNCATGGAGGAACATGACA NGG 2 0.2 Tier II
16 NC_000015.10 15 28002333 + OCA2 NNNCATGGAGCTACAGGGCA NGG 2 0.1385 Tier II
17 NC_000011.10 11 10768570 - CTR9 NNNCATGCAGCTCCATGACA NGG 2 0.1296 Tier II
18 NC_000022.11 22 37380890 - ELFN2 NNNCAGGGAGCTCCATGACA NGG 2 0.1203 Tier II
19 NC_000020.11 20 19969620 - RIN2 NNNCATGGAGCTCCATGTCA NGG 2 0.1053 Tier II
20 NC_000003.12 3 10434703 - ATP2B2 NNNCCTGGAGCTACTTGACA NGG 2 0.1 Tier II
21 NC_000010.11 10 866485 + LARP4B NNNCATGGAGCTACATGAGG NGG 2 0.0956 Tier II
22 NC_000016.10 16 24266321 - CACNG3 NNNCATGGAGCTAAATGACA NAG 2 0.0907 Tier II
23 NC_000017.11 17 74990663 + CDR2L NNNCATGGAGCGACATGAGA NGG 2 0.0625 Tier II
24 NC_000007.14 7 148048291 - CNTNAP2 NNNCATGCAGCTACATGACA NTG 2 0.024 Tier II
25 NC_000019.10 19 57544213 + ZNF550 NNNCATGGAGCTAAATGACA NGT 2 0.0056 Tier II
26 NC_000010.11 10 4399790 + LINC00703 NNNAAAGGAGCTACATGACA NGG 2 0.7298 Tier III
27 NC_000010.11 10 4399790 + LOC105376372 NNNAAAGGAGCTACATGACA NGG 2 0.7298 Tier III
28 NC_000008.11 8 8462722 + LOC107986913 NNNCATGAAGCTGCATGACA NGG 2 0.6522 Tier III
29 NC_000017.11 17 68122199 - LINC00674 NNNCATGAAGCTGCATGACA NGG 2 0.6522 Tier III
30 NC_000004.12 4 96399755 - LINC02267 NNNCATGGTGCTACAAGACA NGG 2 0.5455 Tier III
31 NC_000005.10 5 54388221 + LINC01033 NNNTATTGAGCTACATGACA NGG 2 0.4571 Tier III
32 NC_000022.11 22 37380890 - LOC124905114 NNNCAGGGAGCTCCATGACA NGG 2 0.1203 Tier III
33 NC_000003.12 3 178369264 - LOC105374235 NNNCATGGAGGTACATGACA NAG 2 0.0648 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 136866888 - Ppp3ca NNNCATGAAGCTACATGACA NGG 1 1.0 Tier II
2 NC_000073.6 7 79692796 + Ticrr NNNCACAGAGCTACATGACA NGG 2 0.9091 Tier II
3 NC_000071.6 5 93167320 - Septin11 NNNCATGGCACTACATGACA NGG 2 0.5333 Tier II
4 NC_000085.6 19 39535170 - Cyp2c39 NNNCATGGAGCTACATGATA NGG 1 0.4615 Tier II
5 NC_000083.6 17 28875864 - Pnpla1 NNNCCTGGAGCTATATGACA NGG 2 0.3667 Tier II
6 NC_000074.6 8 87788396 + Zfp423 NNNCATGGAGCTACAAGGCA NGG 2 0.3636 Tier II
7 NC_000076.6 10 28523001 - Ptprk NNNCATGGAGCTACATGATG NGG 2 0.3529 Tier II
8 NC_000084.6 18 64653898 + Atp8b1 NNNCATGGAGCTACAGAACA NGG 2 0.3231 Tier II
9 NC_000068.7 2 20233890 + Etl4 NNNCATGGAGGTACATGACA NGG 1 0.25 Tier II
10 NC_000076.6 10 9325932 - Samd5 NNNCATGGGGCTAAATGACA NGG 2 0.2333 Tier II
11 NC_000081.6 15 91418659 - Slc2a13 NNNCATGGAGCTACTTGACA NGG 1 0.2 Tier II
12 NC_000074.6 8 109814421 - Ap1g1 NNNCATGGAGCTACATGCCA NGG 1 0.1905 Tier II
13 NC_000070.6 4 103705166 + Dab1 NNNCATGGAGCTACATGGAA NGG 2 0.1714 Tier II
14 NC_000072.6 6 141336664 - Pde3a NNNCATTGAGCTTCATGACA NGG 2 0.1714 Tier II
15 NC_000083.6 17 73205334 + Lclat1 NNNCATGGAGCTACGTGACA NAG 2 0.1685 Tier II
16 NC_000071.6 5 112831032 - Myo18b NNNCTTGGACCTACATGACA NGG 2 0.1455 Tier II
17 NC_000068.7 2 125388869 + Fbn1 NNNCATGGAGCTACTTGTCA NGG 2 0.1 Tier II
18 NC_000086.7 X 73003754 - Zfp185 NNNCATGGAGGTACATGGCA NGG 2 0.1 Tier II
19 NC_000068.7 2 114653129 - Dph6 NNNGATGGAGCTACATGAGA NGG 2 0.0625 Tier II
20 NC_000083.6 17 24756291 + Hs3st6 NNNCATGGAGCTACTTGACA NAG 2 0.0519 Tier II
21 NC_000067.6 1 115721184 + Cntnap5a NNNCATGGAGCTACATGCCA NAG 2 0.0494 Tier II
22 NC_000076.6 10 85411171 + Btbd11 NNNCATGGAGCTACATGATA NGA 2 0.0321 Tier II
23 NC_000083.6 17 6550579 + Gm29721 NNNCATGGAGCTACATGGCA NGA 2 0.0278 Tier II
24 NC_000083.6 17 6700610 - Sytl3 NNNCATGGAGCTACATGGCA NGA 2 0.0278 Tier II
25 NC_000084.6 18 44386834 - Dcp2 NNNCAAGGAGCTACATGACA NGT 2 0.014 Tier II
26 NC_000079.6 13 70240202 + 4930520P13Rik NNNCATGGAGCCACAAGACA NGG 2 0.8612 Tier III
27 NC_000075.6 9 115656373 - Gm39448 NNNCAAGGAGCCACATGACA NGG 2 0.8211 Tier III
28 NC_000069.6 3 135475883 - Gm30992 NNNCATGGAGCTGCATGATA NGG 2 0.301 Tier III
29 NC_000074.6 8 127636298 - Gm32478 NNNAATGGAGCTACTTGACA NGG 2 0.1684 Tier III
30 NC_000083.6 17 6383699 + Gm29719 NNNCATGGAGCTACATGGCA NGA 2 0.0278 Tier III
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Other clones with same target sequence:

(none)