Construct: sgRNA BRDN0001162471
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGGAAGACGTCCCCCGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK6 (5753)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76491
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63537223 |
+ |
PTK6 |
NNNGGAAGACGTCCCCCGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
44002409 |
+ |
ACCSL |
NNNGGAAGAAGCCCCCCGCG |
NGG |
2 |
0.8211 |
Tier II |
| 3 |
NC_000002.12 |
2 |
238296959 |
- |
PER2 |
NNNTGAAGATGTCCCCCGCG |
NGG |
2 |
0.3422 |
Tier II |
| 4 |
NC_000019.10 |
19 |
12364822 |
- |
ZNF442 |
NNNGGAAGGCGGCCCCCGCG |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000007.14 |
7 |
151754240 |
- |
PRKAG2 |
NNNGGAAGACGTCCCCAGCC |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000002.12 |
2 |
238296959 |
- |
LOC112268433 |
NNNTGAAGATGTCCCCCGCG |
NGG |
2 |
0.3422 |
Tier III |
| 7 |
NC_000010.11 |
10 |
21496216 |
+ |
MIR1915HG |
NNNGGAAGAGGTCCCCCGCG |
NCG |
2 |
0.0417 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
51419854 |
+ |
S1pr3 |
NNNGGAAGACCTGCCCCGCG |
NGG |
2 |
0.0584 |
Tier I |
| 2 |
NC_000076.6 |
10 |
62559253 |
- |
Kifbp |
NNNGGAAGACGTCCTCCGCC |
NGG |
2 |
0.0286 |
Tier I |
| 3 |
NC_000076.6 |
10 |
80003236 |
- |
Abca7 |
NNNTGAAGACGTCCTCCGCG |
NGG |
2 |
0.0242 |
Tier I |
| 4 |
NC_000085.6 |
19 |
59345466 |
+ |
Pdzd8 |
NNNGGAAGCCGTCGCCCGCG |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000069.6 |
3 |
127499175 |
+ |
Ank2 |
NNNGGGAGACGTCCCCCGGG |
NGG |
2 |
0.0893 |
Tier II |
Other clones with same target sequence:
(none)
