Construct: sgRNA BRDN0001162474
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCAGGAAGCCAATCCGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK2 (80025)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76810
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
3889327 |
- |
PANK2 |
NNNAGGAAGCCAATCCGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
133197004 |
+ |
OPCML |
NNNAGGAAGCCAATCCAACT |
NGG |
2 |
0.6533 |
Tier II |
| 3 |
NC_000003.12 |
3 |
193242384 |
+ |
PLAAT1 |
NNNAGGAAGACAATCCGACC |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000012.12 |
12 |
86639890 |
- |
MGAT4C |
NNNAGGAAGCCAATCAGAGG |
NGG |
2 |
0.125 |
Tier II |
| 5 |
NC_000006.12 |
6 |
20720107 |
- |
CDKAL1 |
NNNAGGAAGCCAATCCCATG |
NGG |
2 |
0.1086 |
Tier II |
| 6 |
NC_000020.11 |
20 |
63738576 |
- |
LIME1 |
NNNAGGAAGCCAACCCGAGG |
NGG |
2 |
0.0357 |
Tier II |
| 7 |
NC_000003.12 |
3 |
193242384 |
+ |
MGC2889 |
NNNAGGAAGACAATCCGACC |
NGG |
2 |
0.3714 |
Tier III |
| 8 |
NC_000023.11 |
X |
22492813 |
- |
PTCHD1-AS |
NNNAGGAAGCCATTCCCACG |
NGG |
2 |
0.0706 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
66678104 |
- |
Uchl1 |
NNNAGGAAGCCAATCTGACA |
NGG |
2 |
0.2885 |
Tier II |
| 2 |
NC_000076.6 |
10 |
82997198 |
+ |
Chst11 |
NNNAGGAAACCAATCCCACG |
NGG |
2 |
0.1513 |
Tier II |
| 3 |
NC_000067.6 |
1 |
71327341 |
- |
Abca12 |
NNNAGGAAGCCAACCCGAGG |
NGG |
2 |
0.0357 |
Tier II |
| 4 |
NC_000070.6 |
4 |
127988346 |
- |
Csmd2 |
NNNAGGAAGCCAAGCCGAGG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000071.6 |
5 |
121191111 |
+ |
Ptpn11 |
NNNGGGAAGCCAAGCCGACG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000067.6 |
1 |
71327341 |
- |
Gm39659 |
NNNAGGAAGCCAACCCGAGG |
NGG |
2 |
0.0357 |
Tier III |
Other clones with same target sequence:
(none)
