Construct: sgRNA BRDN0001162499
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTTATAAACACAGGGTCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80245
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
119800734 |
+ |
CIT |
NNNAAGAAACACAGGGTCGC |
NGG |
2 |
0.2721 |
Tier II |
| 2 |
NC_000015.10 |
15 |
41967324 |
- |
EHD4 |
NNNTAGAAACACAGGGTGGC |
NGG |
2 |
0.0762 |
Tier II |
| 3 |
NC_000001.11 |
1 |
41478806 |
+ |
EDN2 |
NNNTATAAACACAAGTTCGC |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
149750475 |
- |
B3glct |
NNNCAGAAACACAGGGTCGC |
NGG |
2 |
0.3697 |
Tier I |
| 2 |
NC_000071.6 |
5 |
149631158 |
- |
Hsph1 |
NNNTAAAAACACAGGGTAGC |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000081.6 |
15 |
80094576 |
+ |
Syngr1 |
NNNTAAAAAGACAGGGTCGC |
NGG |
2 |
0.337 |
Tier II |
| 4 |
NC_000077.6 |
11 |
105096810 |
+ |
Efcab3 |
NNNTATAAACACAAGGTCCC |
NGG |
2 |
0.3362 |
Tier II |
| 5 |
NC_000076.6 |
10 |
84968616 |
- |
Ric8b |
NNNTATGAACACATGGTCGC |
NGG |
2 |
0.1882 |
Tier II |
| 6 |
NC_000080.6 |
14 |
25842569 |
- |
Anxa11 |
NNNTATAAACCCAGGGTGGC |
NGG |
2 |
0.0533 |
Tier II |
| 7 |
NC_000074.6 |
8 |
41313625 |
- |
Pcm1 |
NNNTTTAAACACAGGGTGGC |
NGG |
2 |
0.0485 |
Tier II |
| 8 |
NC_000071.6 |
5 |
149750475 |
- |
Gm36141 |
NNNCAGAAACACAGGGTCGC |
NGG |
2 |
0.3697 |
Tier III |
Other clones with same target sequence:
(none)
