Construct: sgRNA BRDN0001487042

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGGCACTAGAAGTTTTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80241

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 106868423 - PIK3CG NNNCACTGGAAGTTTTTGAT NGG 2 0.44 Tier I
2 NC_000006.12 6 53900918 + LRRC1 NNNCAAAAGAAGTTTTTGAA NGG 2 0.8125 Tier II
3 NC_000012.12 12 26604381 - ITPR2 NNNCACTGGAAATTTTTGAA NGG 2 0.6844 Tier II
4 NC_000003.12 3 63376365 - SYNPR NNNCATTAGAGGTTTTTGAA NGG 2 0.6036 Tier II
5 NC_000004.12 4 184769212 + ACSL1 NNNAACTAGAAGTTTCTGAA NGG 2 0.5614 Tier II
6 NC_000004.12 4 169462593 + NEK1 NNNCACTAGAAGCTTTTTAA NGG 2 0.5263 Tier II
7 NC_000011.10 11 106847712 + GUCY1A2 NNNCCCTAGAAATTTTTGAA NGG 2 0.4667 Tier II
8 NC_000003.12 3 169179633 + MECOM NNNCACTAAAAGTTTTTAAA NGG 2 0.4451 Tier II
9 NC_000013.11 13 30580194 - HMGB1 NNNCACTAGAACTTTTTAAA NGG 2 0.3665 Tier II
10 NC_000023.11 X 138697653 + FGF13 NNNAACTAGAATTTTTTGAA NGG 2 0.3239 Tier II
11 NC_000001.11 1 169531795 - F5 NNNCACTAGAAGTTATAGAA NGG 2 0.3088 Tier II
12 NC_000017.11 17 48790609 + TTLL6 NNNTTCTAGAAGTTTTTGAA NGG 2 0.2909 Tier II
13 NC_000007.14 7 21605561 - DNAH11 NNNGACTAGGAGTTTTTGAA NGG 2 0.2778 Tier II
14 NC_000013.11 13 40583313 + FOXO1 NNNCACTAGAATTTTCTGAA NGG 2 0.2564 Tier II
15 NC_000006.12 6 20812946 + CDKAL1 NNNCACTAGCAGTTTTTGAG NGG 2 0.2549 Tier II
16 NC_000006.12 6 106248346 + ATG5 NNNCTCTAGAAGTTTCTGAA NGG 2 0.2424 Tier II
17 NC_000003.12 3 186985485 + ST6GAL1 NNNCTCTAAAAGTTTTTGAA NGG 2 0.2338 Tier II
18 NC_000002.12 2 26280358 - HADHB NNNCAATAGAAGTTTTTGAC NGG 2 0.211 Tier II
19 NC_000012.12 12 42496422 + PRICKLE1 NNNCCCTAGAATTTTTTGAA NGG 2 0.1923 Tier II
20 NC_000013.11 13 35398784 + NBEA NNNCCCTAGAATTTTTTGAA NGG 2 0.1923 Tier II
21 NC_000005.10 5 115599909 + TICAM2 NNNCACCAGAAGGTTTTGAA NGG 2 0.1793 Tier II
22 NC_000005.10 5 115599909 + TMED7-TICAM2 NNNCACCAGAAGGTTTTGAA NGG 2 0.1793 Tier II
23 NC_000021.9 21 43513045 - HSF2BP NNNCACTAGAATTTTTTGGA NGG 2 0.1442 Tier II
24 NC_000016.10 16 70314813 - DDX19B NNNCACTAGAAGTTTTTGTA NAG 2 0.1396 Tier II
25 NC_000023.11 X 12643524 + FRMPD4 NNNCATTAGAAGTTTTTGAA NGA 2 0.0645 Tier II
26 NC_000004.12 4 102289146 - SLC39A8 NNNCCCTAGAAGTTTTGGAA NGG 2 0.0588 Tier II
27 NC_000010.11 10 102513785 - SUFU NNNCACTAGAAGTTTTTGAA NTA 2 0.0 Tier II
28 NC_000001.11 1 23103534 - LUZP1 NNNCATTAGAAGTGTTTGAA NGG 2 0.0 Tier II
29 NC_000008.11 8 10476195 - LINC03022 NNNTAATAGAAGTTTTTGAA NGG 2 0.7429 Tier III
30 NC_000008.11 8 10476195 - LOC124901887 NNNTAATAGAAGTTTTTGAA NGG 2 0.7429 Tier III
31 NC_000011.10 11 56620350 - OR5AM1P NNNCACTATAAATTTTTGAA NGG 2 0.4978 Tier III
32 NC_000003.12 3 117038744 - LOC124909415 NNNCACTTGAAGTTTTTGAA NGG 1 0.4286 Tier III
33 NC_000003.12 3 186985485 + LOC124906314 NNNCTCTAAAAGTTTTTGAA NGG 2 0.2338 Tier III
34 NC_000003.12 3 70075664 - SAMMSON NNNCACTAGTAGGTTTTGAA NGG 2 0.2302 Tier III
35 NC_000018.10 18 64896879 + LOC101927404 NNNCACTAGGATTTTTTGAA NGG 2 0.2137 Tier III
36 NC_000018.10 18 64896879 + LOC107985178 NNNCACTAGGATTTTTTGAA NGG 2 0.2137 Tier III
37 NC_000006.12 6 97372965 - LOC101927314 NNNCCCTAGAATTTTTTGAA NGG 2 0.1923 Tier III
38 NC_000022.11 22 34813339 - LINC02885 NNNCACTAGAAGTTTCTGCA NGG 2 0.1379 Tier III
39 NC_000002.12 2 37719882 + LOC105374465 NNNCACTTGATGTTTTTGAA NGG 2 0.1319 Tier III
40 NC_000014.9 14 44228831 - LINC02307 NNNCACTAGAAGTTTTTGAA NTT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 50187082 + Nell1 NNNAACAAGAAGTTTTTGAA NGG 2 0.7368 Tier II
2 NC_000076.6 10 90193893 + Anks1b NNNAACTAGAAGCTTTTGAA NGG 2 0.6648 Tier II
3 NC_000082.6 16 45816509 - Phldb2 NNNAACTAGAAGTTTTTTAA NGG 2 0.5614 Tier II
4 NC_000068.7 2 142387098 + Macrod2 NNNCACTAGAAGTTTTAGAA NGG 1 0.5333 Tier II
5 NC_000071.6 5 98622882 + Cfap299 NNNCACTGGAAGTTTTCGAA NGG 2 0.5176 Tier II
6 NC_000076.6 10 110401935 - Nav3 NNNCACTGGAAGTTTTTAAA NGG 2 0.5077 Tier II
7 NC_000072.6 6 66916168 + Gng12 NNNCACTAGTACTTTTTGAA NGG 2 0.4671 Tier II
8 NC_000072.6 6 146865993 + Smco2 NNNCACTAGAAGATTTTTAA NGG 2 0.4615 Tier II
9 NC_000075.6 9 80456569 + Impg1 NNNCACTGGAACTTTTTGAA NGG 2 0.3882 Tier II
10 NC_000073.6 7 82709716 - Efl1 NNNCACTTGAAGTTTTTAAA NGG 2 0.2967 Tier II
11 NC_000069.6 3 98384915 - Zfp697 NNNCACTAGAAATTCTTGAA NGG 2 0.2545 Tier II
12 NC_000077.6 11 86948631 + Ypel2 NNNCACTAAATGTTTTTGAA NGG 2 0.1978 Tier II
13 NC_000075.6 9 82950840 + Phip NNNGACTAGAAGTCTTTGAA NGG 2 0.1429 Tier II
14 NC_000067.6 1 134593826 - Kdm5b NNNCACTAGAAGTTTTTGTA NAG 2 0.1396 Tier II
15 NC_000071.6 5 35944709 - Afap1 NNNCACTAGAAGTCTTTGAA NGA 2 0.0198 Tier II
16 NC_000071.6 5 128174322 + Tmem132d NNNGACTAGAAGTTTTTGAA NTG 2 0.0195 Tier II
17 NC_000072.6 6 66916168 + 4930597O21Rik NNNCACTAGTACTTTTTGAA NGG 2 0.4671 Tier III
18 NC_000072.6 6 55556719 - Gm34441 NNNCACTAGAAGTCTTTGGA NGG 2 0.1071 Tier III
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Other clones with same target sequence:

(none)