Construct: sgRNA BRDN0001487065
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGAAGCTCGATAATACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKN2 (5586)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77928
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
88786137 |
+ |
PKN2 |
NNNGAAGCTCGATAATACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
31426007 |
+ |
SERINC2 |
NNNGAAGCTAGATAAAACTG |
NGG |
2 |
0.7879 |
Tier II |
| 3 |
NC_000009.12 |
9 |
122294201 |
+ |
MRRF |
NNNGAAGCTCCAAAATACTG |
NGG |
2 |
0.2967 |
Tier II |
| 4 |
NC_000007.14 |
7 |
144404217 |
- |
NOBOX |
NNNGAAGCTCCTTAATACTG |
NGG |
2 |
0.1429 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
142820614 |
- |
Pkn2 |
NNNGAAGCTAGATAATACTG |
NGG |
1 |
0.8667 |
Tier I |
| 2 |
NC_000083.6 |
17 |
70518930 |
+ |
Dlgap1 |
NNNGAAGCTTGAAAATACTG |
NGG |
2 |
0.6516 |
Tier II |
| 3 |
NC_000068.7 |
2 |
141950353 |
- |
Macrod2 |
NNNGAAGCTCTAAAATACTG |
NGG |
2 |
0.2663 |
Tier II |
| 4 |
NC_000068.7 |
2 |
141147637 |
- |
Macrod2 |
NNNGAAGCTCGAGAAGACTG |
NGG |
2 |
0.0903 |
Tier II |
| 5 |
NC_000082.6 |
16 |
33094375 |
+ |
Lmln |
NNNGAATCTCGATAATACTG |
NGT |
2 |
0.0092 |
Tier II |
| 6 |
NC_000067.6 |
1 |
52343436 |
- |
Gm31955 |
NNNGAAGTTTGATAATACTG |
NGG |
2 |
0.8235 |
Tier III |
Other clones with same target sequence:
(none)
