Construct: sgRNA BRDN0001487076

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAGTTACTCAGGTGATAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NADK (65220)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78072

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 1755379 - NADK NNNGTTACTCAGGTGATAGA NGG 0 1.0 Tier I
2 NC_000003.12 3 99642230 - COL8A1 NNNGTTACTAAAGTGATAGA NGG 2 0.8089 Tier II
3 NC_000009.12 9 89374575 - SEMA4D NNNGTTACAAAGGTGATAGA NGG 2 0.8048 Tier II
4 NC_000019.10 19 50676533 + SHANK1 NNNGTTAGACAGGTGATAGA NGG 2 0.5969 Tier II
5 NC_000019.10 19 37091827 + ZNF420 NNNGTTACTCAGGAGATTGA NGG 2 0.3095 Tier II
6 NC_000001.11 1 176771899 - PAPPA2 NNNGTTACTCAGCTGATAAA NGG 2 0.3008 Tier II
7 NC_000002.12 2 63269185 + WDPCP NNNGTTACTTAGTTGATAGA NGG 2 0.2824 Tier II
8 NC_000002.12 2 1353911 + SNTG2 NNNGTCACTCTGGTGATAGA NGG 2 0.2797 Tier II
9 NC_000015.10 15 44196808 - FRMD5 NNNGTTACTAAGGTGATAGA NAG 2 0.2247 Tier II
10 NC_000011.10 11 20713204 + NELL1 NNNGTTTCTCAGGTGATTGA NGG 2 0.2188 Tier II
11 NC_000002.12 2 189997326 + C2orf88 NNNGTTTCTCAGGTGATGGA NGG 2 0.175 Tier II
12 NC_000006.12 6 97244303 + MMS22L NNNGTTTCTCAGGTGATGGA NGG 2 0.175 Tier II
13 NC_000012.12 12 25135975 - DNAI7 NNNGTTTCTCAGGTGATGGA NGG 2 0.175 Tier II
14 NC_000014.9 14 79829379 - NRXN3 NNNGTTCCTCAGTTGATAGA NGG 2 0.1313 Tier II
15 NC_000003.12 3 192894769 - MB21D2 NNNGCTACTCAGGTGAGAGA NGG 2 0.1176 Tier II
16 NC_000011.10 11 7535753 + PPFIBP2 NNNGTTACTCAGGTGAGAGG NGG 2 0.09 Tier II
17 NC_000011.10 11 7535753 + LOC124902806 NNNGTTACTCAGGTGAGAGG NGG 2 0.09 Tier II
18 NC_000005.10 5 55657717 - SLC38A9 NNNCTTACTCAGGTTATAGA NGG 2 0.0756 Tier II
19 NC_000023.11 X 19440260 - MAP3K15 NNNGTTACACAGGTGATAGA NGA 2 0.0645 Tier II
20 NC_000018.10 18 66129 + TUBB8B NNNGTTACTCAGGTGACAGA NGA 2 0.049 Tier II
21 NC_000007.14 7 107231524 + COG5 NNNGCTACTCAGGTGATAGA NTG 2 0.039 Tier II
22 NC_000001.11 1 29179950 - SRSF4 NNNGTTACTCAGGTGATAGT NTG 2 0.0234 Tier II
23 NC_000001.11 1 29179950 - MECR NNNGTTACTCAGGTGATAGT NTG 2 0.0234 Tier II
24 NC_000017.11 17 15315469 + TEKT3 NNNGTTACTCAGGTGAGAGA NGA 2 0.0082 Tier II
25 NC_000005.10 5 66049325 - ERBIN NNNGATACTCAGGTGCTAGA NGG 2 0.0 Tier II
26 NC_000014.9 14 100231466 + YY1-DT NNNGTTACACAGGTGATAAA NGG 2 0.6633 Tier III
27 NC_000006.12 6 137832289 + WAKMAR2 NNNGTGACTCAGATGATAGA NGG 2 0.5275 Tier III
28 NC_000006.12 6 68255719 + LINC02549 NNNGTTTCTCAGGTGATAGG NGG 2 0.3346 Tier III
29 NC_000003.12 3 180697562 + CCDC39-AS1 NNNGTTACTCAGTAGATAGA NGG 2 0.1857 Tier III
30 NC_000007.14 7 26106945 - LOC105375198 NNNTATACTCAGGTGATAGA NGG 2 0.1818 Tier III
31 NC_000007.14 7 26106945 - LOC105375199 NNNTATACTCAGGTGATAGA NGG 2 0.1818 Tier III
32 NC_000012.12 12 95844160 + SNRPF-DT NNNGTTACACAGGTGGTAGA NGG 2 0.1786 Tier III
33 NC_000006.12 6 81477722 + LOC105377871 NNNGTTAGTCAGGTGGTAGA NGG 2 0.1236 Tier III
34 NC_000007.14 7 107231524 + LOC124901721 NNNGCTACTCAGGTGATAGA NTG 2 0.039 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 155586827 + Nadk NNNGTGAATCAGGTGATAGA NGG 2 0.3714 Tier I
2 NC_000067.6 1 64820089 - Plekhm3 NNNTTTCCTCAGGTGATAGA NGG 2 0.1591 Tier I
3 NC_000077.6 11 23726075 + Pus10 NNNGTTAGACAGGTGATAGA NGG 2 0.5969 Tier II
4 NC_000069.6 3 121798098 - Abcd3 NNNGTTACTCATGTGATAGA NGG 1 0.3846 Tier II
5 NC_000077.6 11 118043846 + Dnah17 NNNGTTACTCAGGTGAAAAA NGG 2 0.381 Tier II
6 NC_000077.6 11 70023045 + Dlg4 NNNGTAACTCATGTGATAGA NGG 2 0.3333 Tier II
7 NC_000068.7 2 77119209 + Ccdc141 NNNGTTACTCAGTTGATAAA NGG 2 0.2143 Tier II
8 NC_000076.6 10 21962032 + Sgk1 NNNGTTGCTCAGTTGATAGA NGG 2 0.2118 Tier II
9 NC_000076.6 10 105414234 - Tmtc2 NNNGATACTCAGGTGATGGA NGG 2 0.2 Tier II
10 NC_000073.6 7 91434650 + Dlg2 NNNTTTCCTCAGGTGATAGA NGG 2 0.1591 Tier II
11 NC_000083.6 17 80413313 - Sos1 NNNGTAACTCAGGTGATAGA NGT 2 0.014 Tier II
12 NC_000083.6 17 80413313 - Arhgef33 NNNGTAACTCAGGTGATAGA NGT 2 0.014 Tier II
13 NC_000074.6 8 61671240 + Palld NNNGTTACTCAGTTGATAGA NTG 2 0.0117 Tier II
14 NC_000068.7 2 73396804 - Gm13707 NNNGTTACCCAGATGATAGA NGG 2 0.8521 Tier III
15 NC_000068.7 2 106039930 - Gm9342 NNNGTTACCAAGGTGATAGA NGG 2 0.8 Tier III
16 NC_000084.6 18 47669916 - Gm5095 NNNGTTATTCAGGTGACAGA NGG 2 0.6176 Tier III
17 NC_000080.6 14 103770309 - Gm35197 NNNGATACTCAGGTGATAAA NGG 2 0.3571 Tier III
18 NC_000072.6 6 144896295 + Gm32124 NNNGTTCCTCAGCTGATAGA NGG 2 0.1842 Tier III
19 NC_000078.6 12 43839568 + Gm33384 NNNGTAACTCAGGTGGTAGA NGG 2 0.1667 Tier III
20 NC_000071.6 5 41853300 + Gm5298 NNNGTTACTGAGGTGATAGA NTG 2 0.0152 Tier III
21 NC_000083.6 17 54368164 - 1700025K24Rik NNNGTTACTCATGTGATAGA NTG 2 0.015 Tier III
22 NC_000074.6 8 61671240 + Gm51575 NNNGTTACTCAGTTGATAGA NTG 2 0.0117 Tier III
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Other clones with same target sequence:

(none)