Construct: sgRNA BRDN0001487084

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCACGAGTAGAGAGCAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCK2 (5106)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78082

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 24098250 + PCK2 NNNACGAGTAGAGAGCAAGA NGG 0 1.0 Tier I
2 NC_000014.9 14 24098250 + NRL NNNACGAGTAGAGAGCAAGA NGG 0 1.0 Tier II
3 NC_000013.11 13 71648414 + DACH1 NNNACGAGAAGAGAGAAAGA NGG 2 0.9286 Tier II
4 NC_000001.11 1 107956243 + VAV3 NNNACGAGTTGAGAGAAAGA NGG 2 0.8824 Tier II
5 NC_000001.11 1 203769910 + LAX1 NNNACGAGTAGGGAACAAGA NGG 2 0.6797 Tier II
6 NC_000012.12 12 18387127 - PIK3C2G NNNATGAGTAGAGAGAAAGA NGG 2 0.6364 Tier II
7 NC_000001.11 1 215865568 + USH2A NNNTGGAGTAGAGAGCAAGA NGG 2 0.3818 Tier II
8 NC_000012.12 12 125108324 - AACS NNNACGAGGACAGAGCAAGA NGG 2 0.2653 Tier II
9 NC_000005.10 5 11427208 + CTNND2 NNNACGAGTTGAGAGCAAGA NAG 2 0.2288 Tier II
10 NC_000002.12 2 201442109 + TRAK2 NNNACGAGCAGAGAGCAAGC NGG 2 0.2098 Tier II
11 NC_000014.9 14 33310452 + NPAS3 NNNATGAGTAGAGAGCAAGA NAG 2 0.165 Tier II
12 NC_000020.11 20 34735031 + NCOA6 NNNACAAGTAGAGATCAAGA NGG 2 0.1429 Tier II
13 NC_000003.12 3 170424506 - CLDN11 NNNACGAGAAGAGAGCTAGA NGG 2 0.1238 Tier II
14 NC_000016.10 16 24292626 - CACNG3 NNNAGGAGTAGAGAGGAAGA NGG 2 0.0923 Tier II
15 NC_000012.12 12 66746597 - GRIP1 NNNACGAGTAGAGAGCAACA NGA 2 0.0311 Tier II
16 NC_000011.10 11 30745489 - LOC101928338 NNNACCAGTAAAGAGCAAGA NGG 2 0.6818 Tier III
17 NC_000009.12 9 26798532 - LOC107987028 NNNAGGAGTAGAAAGCAAGA NGG 2 0.5538 Tier III
18 NC_000001.11 1 71193383 + ZRANB2-DT NNNAAGAGTAGAGAGCCAGA NGG 2 0.1008 Tier III
19 NC_000001.11 1 56043645 - LOC105378737 NNNACGAGTAGAGAGGCAGA NGG 2 0.0271 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 55543636 + Pck2 NNNACGGGTAGAAAGCAAGA NGG 2 0.6516 Tier I
2 NC_000070.6 4 63471853 - Whrn NNNACAAGGAGAGAGCAAGA NGG 2 0.619 Tier II
3 NC_000067.6 1 133417233 + Sox13 NNNAGGAGCAGAGAGCAAGA NGG 2 0.5538 Tier II
4 NC_000085.6 19 8826962 + Hnrnpul2 NNNATGAGTAGAGAGCAAGG NGG 2 0.4866 Tier II
5 NC_000086.7 X 16803487 + Maob NNNATGAGGAGAGAGCAAGA NGG 2 0.3939 Tier II
6 NC_000076.6 10 40781002 + Mettl24 NNNAGGAGGAGAGAGCAAGA NGG 2 0.3714 Tier II
7 NC_000067.6 1 55287128 - Boll NNNTAGAGTAGAGAGCAAGA NGG 2 0.3636 Tier II
8 NC_000071.6 5 108521474 - Cplx1 NNNAAGAGGAGAGAGCAAGA NGG 2 0.3537 Tier II
9 NC_000072.6 6 129565325 - Klre1 NNNAAGAGGAGAGAGCAAGA NGG 2 0.3537 Tier II
10 NC_000077.6 11 81208959 - Asic2 NNNACCTGTAGAGAGCAAGA NGG 2 0.2983 Tier II
11 NC_000082.6 16 74334183 - Robo2 NNNATGTGTAGAGAGCAAGA NGG 2 0.2784 Tier II
12 NC_000072.6 6 105726357 + Cntn4 NNNAAGAGTAGAGAGCAACA NGG 2 0.2562 Tier II
13 NC_000075.6 9 7119770 - Dync2h1 NNNAAGAGTAGAGAGTAAGA NGG 2 0.1758 Tier II
14 NC_000070.6 4 96324501 - Cyp2j11 NNNAGGAGTAGAGAGCAAGA NAG 2 0.1556 Tier II
15 NC_000069.6 3 86431439 - Lrba NNNAAGAGTAGAGAGCAAGA NAG 2 0.1481 Tier II
16 NC_000075.6 9 44834146 + Kmt2a NNNACGAGGAGAGAGCCAGA NGG 2 0.1092 Tier II
17 NC_000072.6 6 145073892 - Bcat1 NNNACCAGTAGAGAGGAAGA NGG 2 0.1049 Tier II
18 NC_000074.6 8 14131708 + Dlgap2 NNNAGGAGTAGAGAGCAAGA NGT 2 0.0097 Tier II
19 NC_000075.6 9 96578184 - Rasa2 NNNAGGAGTAGAGAGCAAGA NGT 2 0.0097 Tier II
20 NC_000081.6 15 93689348 - Gm41387 NNNACAAGTATAGAGCAAGA NGG 2 0.3846 Tier III
21 NC_000079.6 13 56405911 - Gm46418 NNNACGAGGCGAGAGCAAGA NGG 2 0.2063 Tier III
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Other clones with same target sequence:

(none)