Construct: sgRNA BRDN0001487093

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGTGCAATTGAAGTCTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AAK1 (22848)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77936

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 69520963 + AAK1 NNNTGCAATTGAAGTCTCTG NGG 0 1.0 Tier I
2 NC_000011.10 11 62533408 - AHNAK NNNTGCACCTGAAGTCTCTG NGG 2 0.3956 Tier I
3 NC_000010.11 10 58193163 + IPMK NNNTGCAACTGAAGTATCTG NGG 2 0.9231 Tier II
4 NC_000015.10 15 43536758 - PPIP5K1 NNNTGTAATTGAAGACTCTG NGG 2 0.5376 Tier II
5 NC_000020.11 20 38881658 - PPP1R16B NNNTGCAAATGAAGTCACTG NGG 2 0.4952 Tier II
6 NC_000008.11 8 66155257 - TRIM55 NNNTGGAATTGAAGTCTCTT NGG 2 0.35 Tier II
7 NC_000001.11 1 246417035 - SMYD3 NNNTGCAGTTGAAGTCTCTC NGG 2 0.3143 Tier II
8 NC_000004.12 4 69290420 - UGT2B28 NNNTGTAATTGAAGTCTCCG NGG 2 0.2562 Tier II
9 NC_000003.12 3 159838076 + SCHIP1 NNNTGAAATTGAAGCCTCTG NGG 2 0.2532 Tier II
10 NC_000003.12 3 159838076 + IQCJ-SCHIP1 NNNTGAAATTGAAGCCTCTG NGG 2 0.2532 Tier II
11 NC_000006.12 6 64987367 + EYS NNNTGCAACTGAATTCTCTG NGG 2 0.2462 Tier II
12 NC_000002.12 2 24761218 - NCOA1 NNNTGCAACTGAAGTCTCTG NAG 2 0.2393 Tier II
13 NC_000003.12 3 42765064 + CCDC13 NNNTGCACTTGAAGTCACTG NGG 2 0.2286 Tier II
14 NC_000007.14 7 150221252 + ACTR3C NNNTGCAATAGAAGTCTCTG NAG 2 0.2222 Tier II
15 NC_000014.9 14 72196928 - RGS6 NNNAGCAATTTAAGTCTCTG NGG 2 0.1832 Tier II
16 NC_000002.12 2 229971625 + FBXO36 NNNGGCAATTGAATTCTCTG NGG 2 0.1667 Tier II
17 NC_000002.12 2 159626036 + BAZ2B NNNTGCAATTCTAGTCTCTG NGG 2 0.1429 Tier II
18 NC_000003.12 3 44960484 + ZDHHC3 NNNTGCAATTCAAGTCTCTG NAG 2 0.1111 Tier II
19 NC_000023.11 X 7912199 - PNPLA4 NNNTGCACTTGAAGTCTGTG NGG 2 0.0571 Tier II
20 NC_000003.12 3 87843078 + HTR1F NNNTGCAATTGAAGCCGCTG NGG 2 0.0321 Tier II
21 NC_000021.9 21 42420746 + UBASH3A NNNTGCTATTGAAGTCTCTG NGT 2 0.0071 Tier II
22 NC_000001.11 1 218703876 + LOC105372924 NNNTGCAATTGAAGTATCTA NGG 2 0.9375 Tier III
23 NC_000001.11 1 90850969 + LINC02609 NNNTGAAATCGAAGTCTCTG NGG 2 0.4952 Tier III
24 NC_000020.11 20 52442228 + LOC105372666 NNNTGGAAGTGAAGTCTCTG NGG 2 0.3095 Tier III
25 NC_000015.10 15 52858513 - LOC107983981 NNNAGCAAGTGAAGTCTCTG NGG 2 0.2948 Tier III
26 NC_000003.12 3 159838076 + LOC124906299 NNNTGAAATTGAAGCCTCTG NGG 2 0.2532 Tier III
27 NC_000006.12 6 64987367 + LOC105377837 NNNTGCAACTGAATTCTCTG NGG 2 0.2462 Tier III
28 NC_000002.12 2 106703321 + LOC102724744 NNNTGCCTTTGAAGTCTCTG NGG 2 0.1875 Tier III
29 NC_000023.11 X 4632470 + LOC101928201 NNNTGCAATTTAAGTTTCTG NGG 2 0.1183 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 86955130 - Aak1 NNNTGCGATTGAAGTCTCTG NGG 1 0.7059 Tier I
2 NC_000069.6 3 95946051 - Anp32e NNNTGCAGATGAAGTCTCTG NGG 2 0.681 Tier II
3 NC_000080.6 14 36922348 + Ccser2 NNNTGCAAGTGAAATCTCTG NGG 2 0.4643 Tier II
4 NC_000077.6 11 105690679 - Tanc2 NNNAGAAATTGAAGTCTCTG NGG 2 0.4422 Tier II
5 NC_000080.6 14 86665470 - Diaph3 NNNTGCATTTGAAGTATCTG NGG 2 0.4286 Tier II
6 NC_000086.7 X 138735343 + Il1rapl2 NNNTGAATTTGAAGTCTCTG NGG 2 0.398 Tier II
7 NC_000072.6 6 101253570 + Pdzrn3 NNNTGCACATGAAGTCTCTG NGG 2 0.398 Tier II
8 NC_000078.6 12 81270231 + Slc8a3 NNNTGCACCTGAAGTCTCTG NGG 2 0.3956 Tier II
9 NC_000077.6 11 67351020 - Myh13 NNNTGCAAGGGAAGTCTCTG NGG 2 0.3095 Tier II
10 NC_000068.7 2 31329694 - Hmcn2 NNNTGGAAGTGAAGTCTCTG NGG 2 0.3095 Tier II
11 NC_000077.6 11 73033584 - Camkk1 NNNTGCAATTGAAGACACTG NGG 2 0.3088 Tier II
12 NC_000067.6 1 170424985 - Nos1ap NNNTGCCATTGAAGTCTTTG NGG 2 0.2812 Tier II
13 NC_000070.6 4 19119946 + Cnbd1 NNNTGCAATTGAAGTCTCTA NAG 2 0.2431 Tier II
14 NC_000082.6 16 23244988 + St6gal1 NNNTGAAATTGAAGTCTCTG NAG 2 0.2407 Tier II
15 NC_000070.6 4 118475200 - Tie1 NNNTGGATTTGAAGTCTCTG NGG 2 0.2143 Tier II
16 NC_000076.6 10 9877946 + Stxbp5 NNNTGCACTTCAAGTCTCTG NGG 2 0.1837 Tier II
17 NC_000067.6 1 161033859 - Zbtb37 NNNTGCAATTGCAGTCTTTG NGG 2 0.1692 Tier II
18 NC_000072.6 6 104739838 - Cntn6 NNNTGCAATTGAAGTTTCTC NGG 2 0.1319 Tier II
19 NC_000082.6 16 22563712 - Dgkg NNNTGCTATTGAAGCCTCTG NGG 2 0.1193 Tier II
20 NC_000072.6 6 35184543 + Nup205 NNNTGCATTTGAAGTCTCTG NAG 2 0.1111 Tier II
21 NC_000076.6 10 120224560 + Tmbim4 NNNTGCATTTGAAGTCTCTG NAG 2 0.1111 Tier II
22 NC_000082.6 16 94925353 - Kcnj6 NNNTGCACTTGAAGTCTCGG NGG 2 0.1071 Tier II
23 NC_000083.6 17 12186704 + Agpat4 NNNTTCAATTGATGTCTCTG NGG 2 0.09 Tier II
24 NC_000083.6 17 62624364 - Efna5 NNNTGCACTTGAAGTGTCTG NGG 2 0.0659 Tier II
25 NC_000081.6 15 12591584 + Pdzd2 NNNTGCACTTGAAGTCTCTG NGA 2 0.0298 Tier II
26 NC_000080.6 14 115689873 + Gpc5 NNNTGCAATAGAAGTCTCTG NGC 2 0.019 Tier II
27 NC_000080.6 14 68088835 + Nefl NNNTGCAATTGAAGTCTCTT NGT 2 0.0113 Tier II
28 NC_000074.6 8 58397306 - Galntl6 NNNTGCAAGTGAAGTCTCTG NGT 2 0.01 Tier II
29 NC_000072.6 6 62121230 - Ccser1 NNNTGCAATTGCAGTCTCTG NGC 2 0.0058 Tier II
30 NC_000077.6 11 20493175 + Gm39607 NNNTGCAAGTGAAGTTTCTG NGG 2 0.1905 Tier III
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Other clones with same target sequence:

(none)