Construct: sgRNA BRDN0001487130

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAGTTGCTCTGGGGGAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80262

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 108179285 - COL4A6 NNNGGTGCTCCGGGGGAACA NGG 2 0.4267 Tier I
2 NC_000011.10 11 65046535 + NAALADL1 NNNCTTGCTCTGGGGGAACA NAG 2 0.1373 Tier I
3 NC_000016.10 16 12279889 + SNX29 NNNGTTCCTCTGGGGGAACA NGG 1 0.6875 Tier II
4 NC_000019.10 19 31224974 - TSHZ3 NNNGGTGCTCAGGGGGAACA NGG 2 0.48 Tier II
5 NC_000007.14 7 1020116 + C7orf50 NNNGCTGCTCTGCGGGAACA NGG 2 0.4211 Tier II
6 NC_000001.11 1 155313174 - FDPS NNNGTTGCTATGCGGGAACA NGG 2 0.3649 Tier II
7 NC_000007.14 7 157870829 - PTPRN2 NNNGGTGCTCTGGGGGATCA NGG 2 0.32 Tier II
8 NC_000011.10 11 72112599 + ANAPC15 NNNGTTTCTCTGGGGGAAAA NGG 2 0.2449 Tier II
9 NC_000005.10 5 96019451 + CAST NNNCTTGCTCTGGGGGAGCA NGG 2 0.2118 Tier II
10 NC_000008.11 8 6568352 - MCPH1 NNNATTGCTCTGGGGGAACC NGG 2 0.2045 Tier II
11 NC_000001.11 1 86361285 - ODF2L NNNGTTGCTCTGTGGGAACT NGG 2 0.18 Tier II
12 NC_000017.11 17 31417772 + RAB11FIP4 NNNGTTGCTGTGGGGGAATA NGG 2 0.1795 Tier II
13 NC_000019.10 19 45062214 + CLASRP NNNGTTGCTCTGGAGGACCA NGG 2 0.1429 Tier II
14 NC_000007.14 7 48015682 - SUN3 NNNGCTGCTCTGGGGGAAGA NGG 2 0.125 Tier II
15 NC_000023.11 X 6078530 + NLGN4X NNNGTTGCACTGGGGGAAGA NGG 2 0.1161 Tier II
16 NC_000016.10 16 12945844 - SHISA9 NNNGTTGCTCTGGGGGAGCC NGG 2 0.0909 Tier II
17 NC_000016.10 16 24943069 + ARHGAP17 NNNGTTGCTCTGGGGGAAGT NGG 2 0.075 Tier II
18 NC_000006.12 6 162478923 + PRKN NNNGTTGCTCTGGGTGATCA NGG 2 0.0714 Tier II
19 NC_000001.11 1 61435169 + NFIA NNNGTTTCTCTGGGGGAACA NGA 2 0.0397 Tier II
20 NC_000019.10 19 19601128 + PBX4 NNNGTTGCTCTGGGGCAACT NGG 2 0.0 Tier II
21 NC_000010.11 10 52635429 - LOC105378305 NNNGTTTTTCTGGGGGAACA NGG 2 0.5 Tier III
22 NC_000013.11 13 20321717 + LOC105370102 NNNGGTGCTCTGGGGGAACG NGG 2 0.4894 Tier III
23 NC_000009.12 9 135553812 + LOC107987040 NNNGGTGCTCCGGGGGAACA NGG 2 0.4267 Tier III
24 NC_000005.10 5 96019451 + LOC101929710 NNNCTTGCTCTGGGGGAGCA NGG 2 0.2118 Tier III
25 NC_000005.10 5 96019451 + LOC105379096 NNNCTTGCTCTGGGGGAGCA NGG 2 0.2118 Tier III
26 NC_000016.10 16 12945844 - LOC105371092 NNNGTTGCTCTGGGGGAGCC NGG 2 0.0909 Tier III
27 NC_000005.10 5 114615302 + LOC101927078 NNNGTGGCTCTGGGGGAACA NGT 2 0.0092 Tier III
28 NC_000002.12 2 46822093 - LINC01118 NNNGTTGCTCTGGGGGCACA NGC 2 0.0039 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 24660026 - Mocos NNNCTGGCTCTGGGGGAACA NGG 2 0.3025 Tier I
2 NC_000075.6 9 59603532 - Celf6 NNNGTTGCTCTGGGGGGTCA NGG 2 0.0882 Tier I
3 NC_000071.6 5 51819811 - Ppargc1a NNNGTTGCCCAGGGGGAACA NGG 2 0.6923 Tier II
4 NC_000075.6 9 63954776 - Smad6 NNNGTTCTTCTGGGGGAACA NGG 2 0.6016 Tier II
5 NC_000085.6 19 46041842 - Ldb1 NNNGCTGCTCTGGGGGAACT NGG 2 0.6 Tier II
6 NC_000075.6 9 42454032 - Tbcel NNNGGTCCTCTGGGGGAACA NGG 2 0.44 Tier II
7 NC_000078.6 12 28809409 - Eipr1 NNNGTTTCTCTGGAGGAACA NGG 2 0.4286 Tier II
8 NC_000075.6 9 86829038 - Snap91 NNNATTGCTCTGGGGGAGCA NGG 2 0.36 Tier II
9 NC_000078.6 12 66984003 - Mdga2 NNNTTTGCTCTGGGAGAACA NGG 2 0.3422 Tier II
10 NC_000071.6 5 140037858 - Mad1l1 NNNCTTGCTCTGGGGGATCA NGG 2 0.2647 Tier II
11 NC_000083.6 17 47904226 + Foxp4 NNNGCTGCTCTGGGGGAACA NAG 2 0.2593 Tier II
12 NC_000077.6 11 97832534 + Lasp1 NNNTTTCCTCTGGGGGAACA NGG 2 0.25 Tier II
13 NC_000071.6 5 113776286 + Iscu NNNCTTGCTCTGGGGGAAAA NGG 2 0.2269 Tier II
14 NC_000068.7 2 132061167 - Slc23a2 NNNGTTGCTCTGAGGGAACC NGG 2 0.2098 Tier II
15 NC_000077.6 11 70012698 + Acadvl NNNGTTGCTGTGGGGGAAAA NGG 2 0.1667 Tier II
16 NC_000082.6 16 59495262 - Crybg3 NNNGATGCTCTGGGGGAACA NAG 2 0.1296 Tier II
17 NC_000070.6 4 19922311 - Atp6v0d2 NNNGTTGCTCTGGGGAAAGA NGG 2 0.125 Tier II
18 NC_000077.6 11 69617966 + Shbg NNNGTTGCTCTGGGGGAAGA NGG 1 0.125 Tier II
19 NC_000079.6 13 91051742 + Atg10 NNNTTTGCTCTGGTGGAACA NGG 2 0.097 Tier II
20 NC_000068.7 2 143793998 - Pcsk2 NNNGTTGCTCTGGAGGAAGA NGG 2 0.0938 Tier II
21 NC_000070.6 4 47402435 - Tgfbr1 NNNGTTGCTCTGGAGGAAGA NGG 2 0.0938 Tier II
22 NC_000068.7 2 152756491 - Cox4i2 NNNGTTCCTCTGGGGGAAGA NGG 2 0.0859 Tier II
23 NC_000074.6 8 47350165 - Stox2 NNNGTTTCTCTGGGTGAACA NGG 2 0.0816 Tier II
24 NC_000078.6 12 100428330 + Ttc7b NNNGGTGCTCTGGGGGAAGA NGG 2 0.08 Tier II
25 NC_000077.6 11 5967540 + Ykt6 NNNGTTGCTCTGGGGGTACT NGG 2 0.08 Tier II
26 NC_000082.6 16 37795208 + Fstl1 NNNGCTGCTCTGGGGGAACA NTG 2 0.039 Tier II
27 NC_000075.6 9 69385231 - Rora NNNGTTGTTCTGGGGGAACA NTG 2 0.0341 Tier II
28 NC_000078.6 12 102969962 - Unc79 NNNGTTGCTTTGGGGGAACA NGT 2 0.0152 Tier II
29 NC_000071.6 5 140802408 - Gna12 NNNGTTGCTCTGGGGTAAAA NGG 2 0.0 Tier II
30 NC_000067.6 1 180933273 + Gm39715 NNNGATGCTCTGGGAGAACA NGG 2 0.4706 Tier III
31 NC_000075.6 9 86998352 + Gm36539 NNNGTTGCTCTGGGAGATCA NGG 2 0.4706 Tier III
32 NC_000085.6 19 30352761 + Gm36006 NNNGTGGATCTGGGGGAACA NGG 2 0.3714 Tier III
33 NC_000078.6 12 15049026 - 4930448C13Rik NNNGTTGCTCTGGAGGGACA NGG 2 0.1324 Tier III
34 NC_000076.6 10 3512182 - Gm30033 NNNGTTCCTCTGGGGGTACA NGG 2 0.0917 Tier III
35 NC_000068.7 2 152756491 - Gm32057 NNNGTTCCTCTGGGGGAAGA NGG 2 0.0859 Tier III
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Other clones with same target sequence:

(none)