Construct: shRNA TRCN0000001755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020397.x-399s1c1
DNA Barcode:
AGAGCTGTTTGACCGGATAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1D (57118)
Gene Description:
calcium/calmodulin dependent protein kinase ID
Transcript:
RefSeq NM_020397.1 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCTGTTTGACCGGATAGT
Hairpin Sequence:
5'-CCGG-AGAGCTGTTTGACCGGATAGT-CTCGAG-ACTATCCGGTCAAACAGCTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCTGTTTGACCGGATAGTCTCGAGACTATCCGGTCAAACAGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCTGTTTGACCGGATAGTCTCGAGACTATCCGGTCAAACAGCTCT-3'

Other clones with same target sequence:

  1. TRCN0000342578