Construct: shRNA TRCN0000002404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138995.x-177s1c1
DNA Barcode:
CCTATGATGCTTGGACTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO3B (140469)
Gene Description:
myosin IIIB
Transcript:
RefSeq NM_138995.1 (NON-CURRENT)
Match location:
Position 177 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATGATGCTTGGACTTGAA
Hairpin Sequence:
5'-CCGG-CCTATGATGCTTGGACTTGAA-CTCGAG-TTCAAGTCCAAGCATCATAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATGATGCTTGGACTTGAACTCGAGTTCAAGTCCAAGCATCATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATGATGCTTGGACTTGAACTCGAGTTCAAGTCCAAGCATCATAGG-3'

Other clones with same target sequence:

(none)