Construct: shRNA TRCN0000003968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003035.x-3494s1c1
DNA Barcode:
TGGATTAAACTGCATGTCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STIL (6491)
Gene Description:
STIL centriolar assembly protein
Transcript:
RefSeq NM_003035.1 (NON-CURRENT)
Match location:
Position 3494 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATTAAACTGCATGTCATT
Hairpin Sequence:
5'-CCGG-TGGATTAAACTGCATGTCATT-CTCGAG-AATGACATGCAGTTTAATCCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATTAAACTGCATGTCATTCTCGAGAATGACATGCAGTTTAATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATTAAACTGCATGTCATTCTCGAGAATGACATGCAGTTTAATCCA-3'

Other clones with same target sequence:

(none)