Construct: shRNA TRCN0000004320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012173.x-1152s1c1
DNA Barcode:
GTACGGAGACACACTGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO25 (26260)
Gene Description:
F-box protein 25
Transcript:
RefSeq NM_012173.3 (CURRENT)
Match location:
Position 1152 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000004320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACGGAGACACACTGCATTT
Hairpin Sequence:
5'-CCGG-GTACGGAGACACACTGCATTT-CTCGAG-AAATGCAGTGTGTCTCCGTAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACGGAGACACACTGCATTTCTCGAGAAATGCAGTGTGTCTCCGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACGGAGACACACTGCATTTCTCGAGAAATGCAGTGTGTCTCCGTAC-3'

Other clones with same target sequence:

  1. TRCN0000314573