Construct: shRNA TRCN0000005633

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018654.1-235s1c1
DNA Barcode:
GCCTTCATCATCGAGCTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPRC5D (55507)
Gene Description:
G protein-coupled receptor class C group 5 member D
Transcript:
RefSeq NM_018654.1 (CURRENT)
Match location:
Position 235 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005633)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCATCATCGAGCTCAAT
Hairpin Sequence:
5'-CCGG-GCCTTCATCATCGAGCTCAAT-CTCGAG-ATTGAGCTCGATGATGAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCATCATCGAGCTCAATCTCGAGATTGAGCTCGATGATGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCATCATCGAGCTCAATCTCGAGATTGAGCTCGATGATGAAGGC-3'

Other clones with same target sequence:

(none)