Transcript: Human NM_018654.1

Homo sapiens G protein-coupled receptor class C group 5 member D (GPRC5D), mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
GPRC5D (55507)
Length:
1038
CDS:
1..1038

Additional Resources:

NCBI RefSeq record:
NM_018654.1
NBCI Gene record:
GPRC5D (55507)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_018654.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005635 GCATTCTCTACAGATCGTGTA pLKO.1 788 CDS 100% 4.050 5.670 N GPRC5D n/a
2 TRCN0000356403 AGTCTGTTGCAAATCATTATT pLKO_005 409 CDS 100% 15.000 10.500 N GPRC5D n/a
3 TRCN0000356335 AGCATGGAAGGCTCATCTTTA pLKO_005 602 CDS 100% 13.200 9.240 N GPRC5D n/a
4 TRCN0000356336 TCCTTCTCCTGGACGACAATT pLKO_005 367 CDS 100% 13.200 9.240 N GPRC5D n/a
5 TRCN0000356334 CATCGAGCTCAATCAACAAAC pLKO_005 243 CDS 100% 10.800 7.560 N GPRC5D n/a
6 TRCN0000005637 CAGCTCAATGTGGACTTTGTT pLKO.1 493 CDS 100% 5.625 3.938 N GPRC5D n/a
7 TRCN0000005633 GCCTTCATCATCGAGCTCAAT pLKO.1 235 CDS 100% 4.950 3.465 N GPRC5D n/a
8 TRCN0000005634 GCTACTCTTAGCATTTCTCTT pLKO.1 117 CDS 100% 4.950 3.465 N GPRC5D n/a
9 TRCN0000005636 CATCTTTATCACTGTGCTCTT pLKO.1 615 CDS 100% 4.050 2.835 N GPRC5D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_018654.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03597 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03597 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480903 ATGACACTATTGTTAGTTGACTTA pLX_317 40.1% 100% 100% V5 n/a
4 ccsbBroadEn_08537 pDONR223 100% 99.9% 100% None 714C>T n/a
5 ccsbBroad304_08537 pLX_304 0% 99.9% 100% V5 714C>T n/a
6 TRCN0000476211 ACGTAGAAAAATGCAGGTGATTGG pLX_317 35.3% 99.9% 100% V5 714C>T n/a
7 TRCN0000488744 TAGCGAAATGGCGTACTGACCCGC pLX_317 35.3% 99.8% 100% V5 (not translated due to prior stop codon) 714C>T;987T>C n/a
8 TRCN0000488548 GGATCCTGTGTCCACACACGCACT pLX_317 35% 99.5% 99.7% V5 (many diffs) n/a
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