Construct: shRNA TRCN0000008428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023913.2-1987s1c1
DNA Barcode:
CCAAATGTGATCCGCTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ern1 (78943)
Gene Description:
endoplasmic reticulum (ER) to nucleus signalling 1
Transcript:
RefSeq NM_023913.2 (CURRENT)
Match location:
Position 1987 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000008428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATGTGATCCGCTACTTT
Hairpin Sequence:
5'-CCGG-CCAAATGTGATCCGCTACTTT-CTCGAG-AAAGTAGCGGATCACATTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATGTGATCCGCTACTTTCTCGAGAAAGTAGCGGATCACATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATGTGATCCGCTACTTTCTCGAGAAAGTAGCGGATCACATTTGG-3'

Other clones with same target sequence:

(none)